A5057606238- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Insect Utilization and Effects
- Parasite Biology and Host Interactions
- Immune Response and Inflammation
- Blood disorders and treatments
- Neuroscience and Neuropharmacology Research
- Nitric Oxide and Endothelin Effects
- Invertebrate Taxonomy and Ecology
- S100 Proteins and Annexins
- Tryptophan and brain disorders
- Composting and Vermicomposting Techniques
- Vasculitis and related conditions
- Plant and animal studies
- Plant Reproductive Biology
- Receptor Mechanisms and Signaling
- Biochemical Analysis and Sensing Techniques
- Biochemical effects in animals
- Treatment of Major Depression
- Psoriasis: Treatment and Pathogenesis
- Leech Biology and Applications
- Neuroscience of respiration and sleep
- Extracellular vesicles in disease
- Phosphodiesterase function and regulation
- Aquaculture disease management and microbiota
Jagiellonian University
2013-2025
Maj Institute of Pharmacology
2024-2025
Polish Academy of Sciences
2024-2025
Cardiff University
2016
Collegium Medicum in Bydgoszcz
2015
Rzeszów University
2014
While key for pathogen immobilization, neutrophil extracellular traps (NETs) often cause severe bystander cell/tissue damage. This was hypothesized to depend on their prolonged presence in the vasculature, leading cytotoxicity. Imaging of NETs (histones, elastase, DNA) with intravital microscopy blood vessels mouse livers a pathogen-replicative-free environment (endotoxemia) led detection NET proteins attached endothelium months despite early disappearance DNA. Intravascular liver...
Systemic inflammation is a detrimental condition associated with high mortality. However, obese individuals seem to have higher chances of surviving sepsis. To elucidate what immunological differences exist between and lean we studied the course endotoxemia in mice fed high-fat diet (HFD) ob/ob animals. Intravital microscopy revealed that neutrophil extracellular trap (NET) formation liver vasculature negligible sharp contrast their counterparts (ND). Unlike individuals, influx not driven by...
Skin infiltration by neutrophils is a hallmark of the chronic inflammatory skin disease psoriasis, yet mechanisms underlying neutrophil recruitment and positioning in chronically inflamed remain poorly understood. In this study, we demonstrate significant impact total genetic deficiency secretory leukocyte protease inhibitor (SLPI) on migration mouse skin. Without SLPI, displayed an unconventional migratory pattern, characterized altered interactions with vessel walls reduced efficiency...
Neutrophil extracellular traps (NETs) are associated with diseases linked to aberrant coagulation. The blood clotting cascade involves a series of proteases, some which induce NET formation via yet unknown mechanism. We hypothesized that this signaling factor Xa (FXa) activation the protease-activated receptor 2 (PAR2). Our findings revealed NETs can be triggered in vitro by enzymatically active proteases and PAR2 agonists. Intravital microscopy liver vasculature both FXa infusion endogenous...
<title>Abstract</title> Cognitive impairment and dementia significantly affect life quality, with conditions like Alzheimer’s, dementia, Parkinsonian posing treatment difficulties. Nitric oxide (NO﮲) is crucial for brain function, yet its imbalance contributes to neurodegeneration. Typically, NO﮲ supports synaptic activity, but under pathological conditions, oxidative stress triggers harmful peroxynitrite formation. Excessive disrupts protein aggravating CNS disorders. Recent studies...
Supravital species identification of morphologically similar syntopic earthworms inhabiting dung and compost heaps or those from commercial cultures is difficult. The aim the studies was to find out non-invasive species-specific markers for proper segregation earthworm a dense mixed colony waste decomposers. Worms were segregated according external characteristics into Eisenia andrei, fetida, Dendrobaena veneta, left reproduction analysis non-invasively retrieved coelomocyte-containing...
Macrophage extracellular traps (METs) represent a novel defense mechanism in the antimicrobial arsenal of macrophages. However, mechanisms MET formation are still poorly understood and this is at least partially due to lack reliable reproducible models. Thus, we aimed establishing protocol induction by bone marrow-derived macrophages (BMDMs) obtained from cryopreserved then thawed marrow (BM) mouse cells. We report that BMDMs way were morphologically (F4/80+) functionally (expression...
Partial negative allosteric modulators (NAM) of the mGlu5 receptor are an excellent alternative to full antagonists and NAMs because they retain some therapeutic effects and, at same time, have a much broader window. Here, we investigated whether partial NAM, M-5MPEP induced fast sustained antidepressant-like effect, characteristic rapid-acting antidepressant drugs (RAADs) like ketamine, in C57BL/6J mice. caused rapid, dose-dependent action tail suspension test (TST) 60 min after injection....
Stressed earthworms expel coelomic fluid containing several vital cellular and soluble components, thus their post-stress recovery has adaptive value. The present manuscript describes the rates of coelomocytes (amoebocytes eleocytes) two fluorophores (riboflavin 4-methylumbelliferyl β-D-glucuronide, MUG) after experimental extrusion by electrostimulation. Analyses were conducted at time points (from 0.5 hour to 7 weeks) a combination cell counts, spectrofluorimetric measurements riboflavin...
Earthworms play an important role in biomedical research, and some surgical procedures require anesthesia. Anesthetic treatments used so far usually induce convulsive body movements connected with extrusion of coelomocyte-containing coelomic fluid that may affect experimental results. Extensive the expulsion are exploited by immunologists as a method harvesting immunocompetent coelomocytes from worms subjected to mild electrostimulation (4.5V). The aim investigations was find anesthetic...