A5037479593- Hematopoietic Stem Cell Transplantation
- Adenosine and Purinergic Signaling
- Complement system in diseases
- Neonatal Respiratory Health Research
- Inflammasome and immune disorders
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Heme Oxygenase-1 and Carbon Monoxide
- Tissue Engineering and Regenerative Medicine
- Erythrocyte Function and Pathophysiology
- Rabbits: Nutrition, Reproduction, Health
- Sphingolipid Metabolism and Signaling
- Virus-based gene therapy research
- RNA modifications and cancer
- Immune cells in cancer
- Viral Infections and Immunology Research
- Acute Myeloid Leukemia Research
- Reproductive Physiology in Livestock
- Platelet Disorders and Treatments
- Mesenchymal stem cell research
- COVID-19 Impact on Reproduction
- Cardiac Structural Anomalies and Repair
- Rabies epidemiology and control
- Cardiovascular Effects of Exercise
Medical University of Warsaw
2017-2024
Icahn School of Medicine at Mount Sinai
2017-2023
University of Louisville
2015-2022
James Graham Brown Foundation
2015-2021
Cardiovascular Institute of the South
2019
Institute of Cell Biology
2018
Jagiellonian University
2014-2017
Malopolska Higher Vocational School of J. Dietl in Kraków
2017
University of Szczecin
2010-2016
Background: Despite its functional importance in various fundamental bioprocesses, studies of N 6 -methyladenosine (m6A) the heart are lacking. Here, we show that FTO (fat mass and obesity-associated protein), an m6A demethylase, plays a critical role cardiac contractile function during homeostasis, remodeling, regeneration. Methods: We used clinical human samples, preclinical pig mouse models, primary cardiomyocyte cell cultures to study cardiomyocytes. modulated expression by using...
Rationale: Extracellular vesicles (EVs) are tiny membrane-enclosed droplets released by cells through membrane budding or exocytosis. The myocardial reparative abilities of EVs derived from induced pluripotent stem (iPSCs) have not been directly compared with the source iPSCs. Objective: To examine whether iPSC-derived can influence biological functions cardiac in vitro and to compare safety efficacy (iPSC-EVs) iPSCs for repair vivo. Methods Results: Murine were generated, isolated culture...
Exosomes are small membrane-bound vesicles of endocytic origin that actively secreted. The potential exosomes as effective communicators biological signaling in myocardial function has previously been investigated, and a recent explosion exosome research not only underscores their significance cardiac physiology pathology, but also draws attention to methodological limitations studying these extracellular vesicles. In this review, we discuss advances challenges with an emphasis on scientific...
Adeno-associated virus (AAV) has emerged as one of the best tools for cardiac gene delivery due to its cardiotropism, long-term expression, and safety. However, a significant challenge successful clinical use is preexisting neutralizing antibodies (NAbs), which bind free AAVs, prevent efficient transduction, reduce or negate therapeutic effects. Here we describe extracellular vesicle-encapsulated AAVs (EV-AAVs), secreted naturally by AAV-producing cells, superior vector that delivers more...
Abstract Microvesicles (MVs) are membrane-enclosed cytoplasmic fragments released by normal and activated cells that have been described as important mediators of cell-to-cell communication. Although the ability human induced pluripotent stem (hiPSCs) to participate in tissue repair is being increasingly recognized, use hiPSC-derived MVs (hiPSC-MVs) this regard remains unknown. Accordingly, we investigated hiPSC-MVs transfer bioactive molecules including mRNA, microRNA (miRNA), proteins...
Evidence has accumulated that hematopoietic stem progenitor cells (HSPCs) share several markers with the germline, a connection supported by reports prolactin, androgens, and estrogens stimulate hematopoiesis. To address this issue more directly, we tested expression of receptors for pituitary-derived hormones, such as follicle-stimulating hormone (FSH) luteinizing (LH), on purified murine bone marrow (BM) enriched HSPCs functionality these in ex vivo signal transduction studies vitro...
Mobilization of stem cells from bone marrow (BM) into peripheral blood (PB) in response to tissue or organ injury, infections, strenuous exercise, mobilization-inducing drugs is as we postulated result a "sterile inflammation" the BM microenvironment that triggers activation Complement Cascade (ComC). Therefore, became interested role Nlrp3 inflammasome this process and show for first time its ATP-dependent manner orchestrates egress hematopoietic stem/progenitor (HSPCs) well other cells,...
Evidence has accumulated that murine haematopoietic stem/progenitor cells (HSPCs) share several markers with the germline, a connection supported by recent reports pituitary and gonadal sex hormones (SexHs) regulate development of HSPCs. It also been reported human HSPCs, like their counterparts, respond to certain SexHs (e.g. androgens). However, better address effects SexHs, particularly on haematopoiesis, we tested for expression receptors including follicle-stimulating hormone (FSH),...
Pharmacological mobilization of hematopoietic stem progenitor cells (HSPCs) from bone marrow (BM) into peripheral blood (PB) is a result mobilizing agent-induced “sterile inflammation” in the BM microenvironment due to complement cascade (ComC) activation. Here we provide evidence that ATP, as an extracellular nucleotide secreted pannexin-1-dependent manner cells, triggers activation ComC and initiates process. This process augmented P2X7 receptor-dependent manner, P2X7-KO mice are poor...
Fast and efficient homing engraftment of hematopoietic stem progenitor cells (HSPCs) is crucial for positive clinical outcomes from transplantation. We found that this process depends on activation the Nlrp3 inflammasome, both in HSPCs to be transplanted recipient bone marrow (BM) microenvironment. For first time we provide evidence functional deficiency inflammasome or host microenvironment leads defective engraftment. At molecular level, their migration response major BM chemoattractant...
Neuron-like cells derived from adipose tissue-derived stem (ADSCs) have been considered one of the most promising for treatment neurodegenerative diseases and neurotrauma in central nervous system (CNS). Thus far, extensive efforts made to facilitate neuronal differentiation ADSCs, but limited progress has achieved. In present study, we tested possibility using a combination electrical stimulation (ES) with Nurr-1 gene transduction promote ADSCs. The tolerance ADSCs ES was first examined by...
Novel evidence that the mannan-binding lectin pathway of complement activation plays a pivotal role in triggering mobilization hematopoietic stem/progenitor cells by both and coagulation cascades
Nitric oxide (NO) is a gaseous free radical molecule involved in several biological processes related to inflammation, tissue damage, and infections. Based on reports that NO inhibits migration of granulocytes monocytes, we became interested the role inducible synthetase (iNOS) pharmacological mobilization hematopoietic stem/progenitor cells (HSPCs) from bone marrow (BM) into peripheral blood (PB). To address HSPC trafficking, upregulated or downregulated iNOS expression cell lines. Next,...
// Mateusz Adamiak 1 , Sylwia Borkowska Marcin Wysoczynski 2 Malwina Suszynska Magda Kucia 1, 3 Gregg Rokosh Ahmed Abdel-Latif 4 Janina Ratajczak Mariusz Z. Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, KY, USA Molecular Cardiology, Department Regenerative Medicine, Medical Warsaw, Poland Division Cardiovascular Gill Heart Institute, Kentucky, Lexington, Correspondence to: Ratajczak, e-mail: mzrata01@louisville.edu Keywords: Pathology Section, S1P, SDF-1,...
Hematopoietic stem/progenitor cells (HSPCs) reside in the bone marrow (BM) microenvironment and are retained there by interaction of membrane lipid raft-associated receptors, such as α-chemokine receptor CXCR4 α4β1-integrin (VLA-4, very late antigen 4 receptor) receptor, with their respective specific ligands, stromal-derived factor 1 vascular cell adhesion molecule 1, expressed BM stem niches. The integrity rafts containing these receptors is maintained glycolipid...
We have recently demonstrated that purinergic signaling in bone marrow (BM) microenvironment regulates mobilization of hematopoietic stem progenitor cells (HSPCs), mesenchymal stroma (MSCs), endothelial (EPCs), and very small embryonic like (VSELs) into the peripheral blood (PB). While extracellular adenosine triphosphate (ATP) promotes mobilization, its metabolite has an opposite effect. Since ATP is processed space to by ectonucleotidases including cell surface expressed CD39 CD73, we...
Abstract NADPH oxidase 2 (Nox2), a superoxide-generating enzyme, is source of reactive oxygen species (ROS) that regulate the intracellular redox state, self-renewal, and fate hematopoietic stem/progenitor cells (HSPCs). Nox2 complex expressed on HSPCs associated with several activated cell membrane receptors increases level ROS. In addition, ROS are also released from mitochondria and, all together, potent activators pattern recognition receptor Nlrp3 inflammasome, which regulates...
Abstract Like their homing after transplantation to bone marrow (BM), the mobilization of hematopoietic stem/progenitor cells (HSPCs) is still not fully understood, and several overlapping pathways are involved. Several years ago our group proposed that sterile inflammation in BM microenvironment induced by pro-mobilizing agents a driving force this process. In favor proposal, both complement cascade (ComC)-deficient Nlrp3 inflammasome-deficient mice poor G-CSF AMD3100 mobilizers. It also...