Primary cilia are dynamic, complex structures that contain >500 proteins, including several related to polycystic kidney disease. How these proteins target and assemble is unknown. We previously identified Cys1 as the gene responsible for disease in Cys1(cpk) mice, a mouse model of autosomal recessive disease; this encodes cystin, 145-amino acid cilium-associated protein. Here, we characterized localization cystin embryonic liver, isolated renal collecting ducts, an inner medullary duct cell...

Bilobalide, the only sesquiterpene compound from Ginkgo biloba leaf, exhibits various beneficial pharmaceutical activities, such as antioxidant, anti-inflammation, and protective effects for central nervous system. Several bioactive components extracted extract reportedly have potential to attenuate lipid metabolism. However, effect of bilobalide on metabolism remains unclear. In this study, we used 3T3-L1 cells cell model investigate adipogenesis. The results showed that inhibited...

Dihydro-β-ionone is a common type of ionone used in the flavor and fragrance industries because its characteristic scent. The production flavors microbial cell factories offers sustainable environmentally friendly approach to accessing them, independent extraction from natural sources. However, native pathway dihydro-β-ionone remains unclear, hindering heterologous biosynthesis hosts. Herein, we devised platform for de novo syntheses simple carbon source with glycerol. complete was...

Fap1, a serine-rich glycoprotein, is essential for fimbrial biogenesis and biofilm formation of Streptococcus parasanguinis (formerly S. parasanguis). Fap1-like proteins are conserved in many streptococci staphylococci have been implicated bacterial virulence. Fap1 contains two repeat regions that modified by O-linked glycosylation. A seven-gene cluster has identified, this biogenesis. In study, we investigated the initial step glycosylation using recombinant as model. This molecule same...

Mature Fap1, a 200-kDa fimbria-associated adhesin, is required for fimbrial biogenesis and biofilm formation in Streptococcus parasanguis. Fap1-like proteins are found the genomes of many streptococcal staphylococcal species. Fap1 serine-rich glycoprotein modified by O-linked glycan moieties. In this study, we identified seven-gene cluster including secY2, orf1, orf2, orf3, secA2, gtf1, gtf2 that localized immediately downstream fap1. The lower G+C contents presence putative transposase...

Abstract Control of the malaria vector An. gambiae is still largely obtained through chemical intervention using pyrethroids, such as permethrin. However, strains that are resistant to toxic effects pyrethroids have become widespread in several endemic areas over last decade. The objective this study was assess differences five life-history traits (larval developmental time and body weight, fecundity, hatch rate, longevity adult females) energy metabolism between a strain permethrin (RSP),...

Abstract Background Autophagy is a programmed cell degradation mechanism that has been associated with several physiological and pathophysiological processes, including malignancy. Improper induction of autophagy proposed to play pivotal role in the progression hepatocellular carcinoma (HCC). Methods Univariate Cox regression analysis overall survival (OS) was performed identify risk-associated autophagy-related genes (ARGs) HCC data set from The Cancer Genome Atlas (TCGA). Multivariate cox...

Bilobalide exhibits numerous beneficial bioactivities, including neuroprotective, anti-inflammatory, and antioxidant activity. Our previous study demonstrated that bilobalide inhibits adipogenesis promotes lipolysis. The dose-dependent cytotoxicity was found to be specific the mature adipocytes only, indicating potential for regulating apoptosis in them. Herein, we aimed investigate apoptotic effects of on 3T3-L1 elucidate underlying mechanisms thereof. Flow cytometry analysis (FACS)...

Mitochondria are organelles found in nearly all eukaryotic cells that play a crucial role cellular survival and function. Mitochondrial function is under the control of nuclear mitochondrial genomes. While latter has been focus most genetic research, we remain largely ignorant about nuclear-encoded genomic inter-individual variability Here, used Drosophila melanogaster as our model organism to address this question.We quantified state 3 4 respiration rates P:O ratio mitochondria isolated...

Osmanthus fragrans has a long history of cultivation in Asia and is widely used food production for its unique aroma, which important cultural economic values. It rich flavonoids with diverse pharmacological properties, such as antioxidant, anti-tumor, anti-lipid activities. However, little known regarding the effects flavonoid extract (OFFE) on adipogenesis pre-adipocyte transdifferentiation. Herein, this research aimed to investigate effect OFFE differentiation, adipogenesis, beiging...

Cystin is a novel cilia-associated protein that disrupted in the cpk mouse, well-characterized mouse model of autosomal recessive polycystic kidney disease (ARPKD). Interestingly, overexpression Myc gene evident animal models ARPKD and thought to contribute renal cystic phenotype. Using yeast two-hybrid approach, growth suppressor necdin, known modulate expression, was found as an interacting partner cystin. Deletion mapping demonstrated C-terminus cystin both termini necdin are required for...

Abstract Background Cell adhesion molecules (CAMs) are expressed ubiquitously. Each of the four families CAMs is comprised glycosylated, membrane-bound proteins that participate in multiple cellular processes including cell-cell communication, cell motility, inside-out and outside-in signaling, tumorigenesis, angiogenesis metastasis. Intercellular molecule-2 (ICAM-2), a member immunoglobulin superfamily CAMs, has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153,...

The fruit Prunus mume has beneficial effects in the treatment of obesity and metabolic syndrome. However, its mechanism action is unclear.We assessed effect a concentrated water extract P. (CEPM) on adipogenesis beiging/browning 3T3-L1 cells.The cell viability was determined by MTT assay. Lipid accumulation with Oil Red O (ORO) staining under different concentrations CEPM. CEPM during differentiation mitochondrial biogenesis cells were investigated.CEPM suppressed decreased lipid...

The utilization of heterosis in rice, maize and rapeseed has revolutionized crop production. Although elite hybrid cultivars are mainly derived from the F1 crosses between two groups parents, named NCII mating design, little been known about methodology how interacted effects influence quantitative trait performance population. To bridge genetic analysis with breeding, here we integrated an QTL mapping approach breeding by design partial design. All potential main were included one full...

Abstract Introduction To compare the saliva proteomes of experimental Sjögren's syndrome (ESS) model mice and healthy controls to identify potential diagnostic biomarkers for primary (pSS). Methods Proteins were extracted from three ESS normal control using data‐independent acquisition technique. R language was used differentially expressed proteins (DEPs). Gene Ontology Kyoto Encyclopedia Genes Genomes pathway analyses performed functionally annotate DEPs. The protein–protein interaction...

Natural β-ionone, a high-value flavoring agent, has been widely applied in the food, cosmetics, and perfume industry. However, attempts to overproduce β-ionone microorganisms have limited by efficiency of carotenoid cleavage dioxygenases (CCDs), which catalyzes β-carotene biosynthesis pathway. In order obtain CCD genes responsible for specific carotenoids generating novel dioxygenase 1 from Helianthus annuus was cloned overexpressed Escherichia coli BL21(DE3). The recombinant able cleave...

Ganoderma lucidum polysaccharides (GLP) are one of the major bioactive components with many beneficial properties. In present study we aimed to systematically evaluate effects GLP on lipid metabolism in human (HPA-v) and murine adipocytes (3T3-L1). Cell viability was assessed by MTT assay. Lipid accumulation mature were evaluated ORO staining quantified using triglyceride (TG) Lipolysis investigated measuring free glycerol released cell culture medium after treatments. The mRNA protein...

Life is supported by metabolism, a vital physiological activity. It guarantees enough energy available to power every cellular The primary substrate, glucose, where the majority of needed cell support all other life activities and growth living creatures derived. quality greatly impacted any disease or flaw that alters metabolism glucose. There no known cure for metabolic illness as diabetes. As diabetes so varied, only effective treatments would be based on precision medicine molecular...

Abstract Objectives The study was aimed to investigate the effects of Xinfeng capsule (XFC) on tissue morphology, and gland function salivary (SG) in a primary Sjögren’s syndrome (pSS) mouse model. Methods An animal model pSS established by inducing SG protein C57BL/6 mice. tissues were collected for sequencing subsequent experiments detect expression cholinergic receptor muscarinic 3(M3R), early growth response factor 1 (EGR1) target genes before after XFC intervention, with vitro...