A5075067727- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Wheat and Barley Genetics and Pathology
- Plant and fungal interactions
- Indoor Air Quality and Microbial Exposure
- Pesticide Residue Analysis and Safety
- Plant Disease Resistance and Genetics
- Agriculture, Plant Science, Crop Management
- Insect and Pesticide Research
- Plant-Microbe Interactions and Immunity
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Plant Pathogens and Resistance
- Seed and Plant Biochemistry
- Listeria monocytogenes in Food Safety
- Fungal and yeast genetics research
- Carcinogens and Genotoxicity Assessment
- Spectroscopy and Chemometric Analyses
- Analytical Chemistry and Chromatography
- Probiotics and Fermented Foods
- Plant Toxicity and Pharmacological Properties
- Bacillus and Francisella bacterial research
- Odor and Emission Control Technologies
- Mycorrhizal Fungi and Plant Interactions
- Cassava research and cyanide
BOKU University
2016-2025
AgroBio
2022
Austrian Competence Centre of Food Safety
2020
University of Abuja
2016
Universitätsklinikum Tulln
2014-2015
Czech Academy of Sciences, Institute of Analytical Chemistry
2014
Natural Resources Institute Finland
2013
TU Wien
2001-2007
Abstract This paper describes the first validated method for determination of 39 mycotoxins in wheat and maize using a single extraction step followed by liquid chromatography with electrospray ionization triple quadrupole mass spectrometry (LC/ESI‐MS/MS) without need any clean‐up. The analytes included A‐ B‐trichothecenes (including deoxynivalenol‐3‐glucoside), zearalenone related derivatives, fumonisins, enniatins, ergot alkaloids, ochratoxins, aflatoxins moniliformin. large number...
An LC–MS/MS "dilute and shoot" method for the determination of 295 fungal bacterial metabolites was optimized validated according to guidelines established in Directorate General Health Consumer Affairs European Commission (SANCO) document No. 12495/2011. Four different types food matrices were chosen validation: apple puree infants (high water content), hazelnuts fat maize starch low content) green pepper (difficult or unique matrix). Method accuracy precision evaluated using spiked samples...
The development of liquid chromatography-mass spectrometry (LC-MS)/mass (MS) methods for the simultaneous detection and quantification a broad spectrum mycotoxins has facilitated screening larger number samples contamination with wide array less well-known "emerging" other metabolites. In this study, 83 feed raw materials were analysed. All them found to contain seven 69 total detected metabolites amounts 139. Fusarium most common, but Alternaria toxins also occurred very often. Furthermore,...
In this study an LC-MS/MS multitoxin method covering a total of 247 fungal and bacterial metabolites was applied to the analysis different foods feedstuffs from Burkina Faso Mozambique. Overall, 63 were determined in 122 samples mainly maize groundnuts few sorghum, millet, rice, wheat, soy, dried fruits, other processed animal feeds. Aflatoxin B1 observed more frequently (Burkina Faso, 50% incidence, median = 23.6 μg/kg; Mozambique, 46% 69.9 μg/kg) than 22% 10.5 14% 3.4 μg/kg). Fumonisin...
Abstract This paper describes the validation of an LC-MS/MS-based method for quantification > 500 secondary microbial metabolites. Analytical performance parameters have been determined seven food matrices using individual samples per matrix spiking. Apparent recoveries ranged from 70 to 120% 53–83% all investigated analytes (depending on matrix). number increased 84–94% if recovery extraction was considered. The comparison fraction which precision criterion RSD ≤ 20% under repeatability...
Global trade of agricultural commodities (e.g., animal feed) requires monitoring for fungal toxins. Also, little is known about masked and emerging toxins metabolites. 1926 samples from 52 countries were analysed Of 162 compounds detected, up to 68 metabolites found in a single sample. A subset 1113 finished feed, maize silage containing 57 2012 2015 44 was investigated using liquid chromatography mass spectrometry. Deoxynivalenol (DON), zearalenone (ZEN) fumonisins showed large increases...
This study reports on the detailed investigation of human deoxynivalenol (DON) and zearalenone (ZEN) in vivo metabolism through analysis urine samples obtained from one volunteer following a naturally contaminated diet containing 138μg DON 10μg ZEN over period four days. Based mycotoxin intake concentrations conjugates urine, mass balance was established. The average rates excretion glucuronidation were determined to be 68 76%, respectively. formed glucuronides revealed DON-15-glucuronide as...
Deoxynivalenol-3-β-D-glucoside (D3G), a phase II plant metabolite of the mycotoxin deoxynivalenol (DON), occurs in naturally Fusarium-contaminated cereals. In order to investigate frequency occurrence as well relative and absolute concentrations D3G infected cereals, 23 wheat samples originating from fields Austria, Germany Slovakia 54 maize Austrian were analysed for DON by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Both analytes detected all 77 field samples. was found at...
Summary Fungal pathogens provoke devastating losses in agricultural production, contaminate food with mycotoxins and give rise to life‐threatening infections humans. The soil‐borne ascomycete F usarium oxysporum attacks over 100 different crops can cause systemic fusariosis immunocompromised individuals. Here we functionally characterized VeA , VelB VelC LaeA four components of the velvet protein complex which regulates fungal development secondary metabolism. Deletion veA velB a minor...
A multi-target method for the determination of 191 fungal metabolites in almonds, hazelnuts, peanuts and pistachios was developed. The includes all mycotoxins regulated European Union regularly found food. After extraction with an acidified acetonitrile water mixture, raw extract diluted injected directly into UHPLC-MS/MS system. In two chromatographic runs, analysis performed positive negative ionisation mode. in-house validated most important 65 analytes these four commodities. Apparent...
RATIONALE Mycotoxins regularly occur in food worldwide and pose serious health risks to consumers. Since individuals can be exposed a variety of these toxic secondary metabolites fungi at the same time, there is demand for proper analytical methods assess human exposure by suitable biomarkers. METHODS This study reports on development liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) method quantitative measurement 15 mycotoxins key urine using polarity...
Recent climatological research predicts a significantly wetter climate in Southern Norway as result of global warming. Thus, the country has already experienced unusually wet summer seasons last three years (2010-2012). The aim this pilot study was to apply an existing multi-analyte LC-MS/MS method for semi-quantitative determination 320 fungal and bacterial metabolites Norwegian cereal grain samples from 2011 growing season. Such knowledge could provide important information future survey...
The natural co-occurrence of fungal metabolites in maize samples from the South region Brazil was studied using an LC-MS/MS based multi-mycotoxin method. All (n = 148) were contaminated with fumonisin B1 (FB1) and B2 (FB2). Aflatoxin (AFB1) aflatoxin G1 (AFG1) detected 38 11 samples, respectively, while zearalenone (ZEN) deoxynivalenol (DON), which first regulated 2014, found 110 71 respectively. Apart mycotoxins, a broad range non-regulated metabolites, Aspergillus, Fusarium, Alternaria,...