A5041929818- Mycotoxins in Agriculture and Food
- Metabolomics and Mass Spectrometry Studies
- Health, Environment, Cognitive Aging
- Wheat and Barley Genetics and Pathology
- Carcinogens and Genotoxicity Assessment
- Pesticide Residue Analysis and Safety
- Nutritional Studies and Diet
- Plant and fungal interactions
- Insect and Pesticide Research
- Advanced Chemical Sensor Technologies
- Gut microbiota and health
- Fungal Plant Pathogen Control
- Effects and risks of endocrine disrupting chemicals
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Analytical Chemistry and Chromatography
- Plant Pathogens and Resistance
- Advanced Proteomics Techniques and Applications
- Indoor Air Quality and Microbial Exposure
- Agriculture, Plant Science, Crop Management
- Fungal Biology and Applications
- Plant Toxicity and Pharmacological Properties
- Mass Spectrometry Techniques and Applications
- Probiotics and Fermented Foods
- Phytoestrogen effects and research
University of Vienna
2016-2025
Ghent University
2022
TU Wien
2022
University of Buenos Aires
2022
Weatherford College
2022
Scripps Research Institute
2016-2020
BOKU University
2011-2017
Linköping University
2010
METLIN originated as a database to characterize known metabolites and has since expanded into technology platform for the identification of unknown other chemical entities. Through this effort it become comprehensive resource containing over 1 million molecules including lipids, amino acids, carbohydrates, toxins, small peptides, natural products, among classes. METLIN's high-resolution tandem mass spectrometry (MS/MS) database, which plays key role in process, data generated from both...
Many intestinal pathogens, including Clostridioides difficile, use mucus-derived sugars as crucial nutrients in the gut. Commensals that compete with pathogens for such are therefore ecological gatekeepers healthy guts, and attractive candidates therapeutic interventions. Nevertheless, there is a poor understanding of which commensals mucin-derived situ well their potential to impede pathogen colonization. Here, we identify mouse gut utilize monosaccharides within complex communities using...
In this study an LC-MS/MS multitoxin method covering a total of 247 fungal and bacterial metabolites was applied to the analysis different foods feedstuffs from Burkina Faso Mozambique. Overall, 63 were determined in 122 samples mainly maize groundnuts few sorghum, millet, rice, wheat, soy, dried fruits, other processed animal feeds. Aflatoxin B1 observed more frequently (Burkina Faso, 50% incidence, median = 23.6 μg/kg; Mozambique, 46% 69.9 μg/kg) than 22% 10.5 14% 3.4 μg/kg). Fumonisin...
This study reports on the detailed investigation of human deoxynivalenol (DON) and zearalenone (ZEN) in vivo metabolism through analysis urine samples obtained from one volunteer following a naturally contaminated diet containing 138μg DON 10μg ZEN over period four days. Based mycotoxin intake concentrations conjugates urine, mass balance was established. The average rates excretion glucuronidation were determined to be 68 76%, respectively. formed glucuronides revealed DON-15-glucuronide as...
RATIONALE Mycotoxins regularly occur in food worldwide and pose serious health risks to consumers. Since individuals can be exposed a variety of these toxic secondary metabolites fungi at the same time, there is demand for proper analytical methods assess human exposure by suitable biomarkers. METHODS This study reports on development liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) method quantitative measurement 15 mycotoxins key urine using polarity...
Mycotoxins produced by Alternaria fungi are ubiquitous food contaminants, but analytical methods for generating comprehensive exposure data rare. We describe the development of an LC-MS/MS method covering 17 toxins investigating natural occurrence free and modified in tomato sauce, sunflower seed oil, wheat flour. Target analytes included alternariol (AOH), AOH-3-glucoside, AOH-9-glucoside, AOH-3-sulfate, monomethyl ether (AME), AME-3-glucoside, AME-3-sulfate, altenuene, isoaltenuene,...
Mycotoxins are toxic secondary metabolites formed by various fungal species that found as natural contaminants in food. This very heterogeneous group of compounds triggers multiple mechanisms, including endocrine disruptive potential. Current risk assessment mycotoxins, for most chemical substances, is based on the effects single compounds. However, concern a potential enhancement risks interactions substances naturally occurring mixtures has greatly increased recently. In this study,...
Concurrent exposure to a wide variety of xenobiotics and their combined toxic effects can play pivotal role in health disease, yet are largely unexplored. Investigating the totality these exposures, i.e., "exposome", specific biological constitutes new paradigm for environmental but still lacks high-throughput, user-friendly technology. We demonstrate utility mass spectrometry-based global metabolomics with tailored database queries cognitive computing comprehensive assessment...
There is a critical need to better understand the patterns, levels and combinatory effects of exposures we are facing through our diet environment. Mycotoxin mixtures particular concern due chronic low dose caused by naturally contaminated food. To facilitate new insights into their role in disease, mycotoxins metabolites quantified bio-fluids as biomarkers exposure. Here, describe highly sensitive urinary assay based on ultra-high performance liquid chromatography - tandem mass spectrometer...
Abstract Compartmentalization of the gut microbiota is thought to be important system function, but extent spatial organization in ecosystem remains poorly understood. Here, we profile murine colonic along longitudinal and lateral axes using laser capture microdissection. We found fine-scale structuring marked by gradients composition diversity length colon. Privation fiber reduces disrupts composition. Both mucus-adjacent luminal communities are influenced absence dietary fiber, with loss a...
Fusarium graminearum and related species commonly infest grains causing the devastating plant disease head blight (FHB) formation of trichothecene mycotoxins. The most relevant toxin is deoxynivalenol (DON), which acts as a virulence factor pathogen. FHB difficult to control resistance this polygenic trait, mainly mediated by quantitative trait loci (QTL) Fhb1 Qfhs.ifa-5A. In study we established targeted GC–MS based metabolomics workflow comprising standardized experimental setup for...
Non-targeted analysis (NTA) workflows using mass spectrometry are gaining popularity in many disciplines, but universally accepted reporting standards nonexistent. Current guidance addresses limited elements of NTA reporting-most notably, identification confidence-and is insufficient to ensure scientific transparency and reproducibility given the complexity these methods. This lack hinders researchers' development thorough study protocols reviewers' ability efficiently assess grant...