A5044342030- Nanoparticles: synthesis and applications
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- Invertebrate Immune Response Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Epigenetics and DNA Methylation
- Helicobacter pylori-related gastroenterology studies
- Cancer Mechanisms and Therapy
- Genetic factors in colorectal cancer
- Curcumin's Biomedical Applications
- RNA Interference and Gene Delivery
- Healthcare and Environmental Waste Management
- Cancer-related gene regulation
- Monoclonal and Polyclonal Antibodies Research
- Air Quality and Health Impacts
- Insect Resistance and Genetics
- Coal Combustion and Slurry Processing
- Humic Substances and Bio-Organic Studies
- Acute Lymphoblastic Leukemia research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Mechanisms of cancer metastasis
- Advanced biosensing and bioanalysis techniques
- TGF-β signaling in diseases
- Biomarkers in Disease Mechanisms
- Histone Deacetylase Inhibitors Research
Macau University of Science and Technology
2023
National University of Singapore
2014-2021
Chinese University of Hong Kong, Shenzhen
2020-2021
New York Medical College
2012-2020
Background: Although zinc oxide nanoparticles (ZnO NPs) have been widely used, there has an increasing number of reports on the toxicity ZnO NPs. However, study underlying mechanisms under in vivo conditions is insufficient. Methods: In this study, we investigated toxicological profiles NPs MRC5 human lung fibroblasts vitro and model using fruit fly Drosophila melanogaster . A comprehensive was conducted to evaluate uptake, cytotoxicity, reactive oxygen species (ROS) formation, gene...
Reactive oxygen species (ROS) play important roles in fundamental cellular processes such as proliferation and survival. Here we investigated the effect of oxidative stress on stem cell maintenance neuronal differentiation a human embryonic (hESC) model, Ntera2 (NT2). CM-H2DCFDA DHE assays confirmed that oxidizing agent paraquat could induce high level ROS NT2 cells. Quantitative PCR, Western blotting immunocytochemistry showed paraquat-induced suppressed expression stemness markers,...
Oxidative stress influences stem cell behavior by promoting the differentiation, proliferation, or apoptosis of cells. Thus, characterizing effects reactive oxygen species (ROS) on provides insights into significance redox homeostasis in cell-associated diseases and efficient expansion for cellular therapies. We utilized Drosophila testis as an vivo model to examine ROS germline (GSC) maintenance. High levels induced alteration Keap1/Nrf2 activity decreased GSC number precocious...
Dysregulated JAK/STAT signaling has been implicated in breast cancer metastasis, which is associated with high relapse risks. However, mechanisms underlying signaling-mediated tumorigenesis are poorly understood. Here, we showed that GRAMD1B expression upregulated on IL-6 but downregulated upon treatment the JAK2 inhibitor AG490 MDA-MB-231 cells. Notably, Gramd1b knockdown caused morphological changes of cells, characterized by formation membrane ruffling and protrusions, implicating its...
Reactive oxygen species (ROS) are byproducts generated during normal cellular metabolism, and redox states have been shown to influence stem cell self-renewal lineage commitment across phyla. However, the downstream effectors of ROS signaling that control behavior remain largely unexplored. Here, we used Drosophila testis as an in vivo model identify ROS-induced involved differentiation process germline cells (GSCs). In Affymetrix microarray analysis, 152 genes were either upregulated or...
Aims Y‐box binding protein‐1 ( YB ‐1) is known to modulate gene transcription and protein translation, as well cellular response drug treatment. The aim of this study correlate ‐1 expression levels with clinicopathological parameters in intestinal‐type gastric cancer tissue samples (as categorized by the Lauren classification) substantiate findings vitro experimentation. Methods results Paraffin‐embedded from 167 patients were used for construction microarrays TMA s). slides immunostained...
Y-box binding protein-1 (YB-1) facilitates cancer chemoresistance through the upregulation of ATP-binding cassette (ABC) transporters associated with multidrug resistance, which is one primary obstacles in treatment. Since aberrant Janus kinase (JAK)/signal transducer and activator transcription (STAT) signaling also implicated numerous human malignancies, interaction between YB-1 JAK/STAT was explored underlying NUGC3 gastric cells. It demonstrated that translocated into nuclei cells...
Identification of prognostic and predictive biomarkers for breast cancer is essential to better stratify patients treatment evaluate patient outcome. AT-rich interactive domain-containing protein 1B (ARID1B) implicated in cell proliferation, but its role tumorigenesis remains unclear.Immunohistochemical analysis ARID1B expression using tissue microarrays containing 156 invasive ductal carcinoma samples subsequent statistical data based on immunoreactivity score were performed examine the...
Complement component 1, q subcomponent binding protein (C1QBP), is a multi-compartmental with higher mRNA expression reported in breast cancer tissues. This study evaluated the association between immunohistochemical of C1QBP tissue microarrays (TMAs) and clinicopathological parameters, particular tumor size. In addition, an vitro was conducted to substantiate TMA findings. Breast TMAs were constructed from pathological specimens patients diagnosed invasive ductal carcinoma. proliferating...
// Puja Khanna 1 , Pei Jou Chua Belinda Shu Ee Wong Changhong Yin 2 Aye Thike 3 Wei Keat Wan 3, 4 Puay Hoon Tan and Gyeong Hun Baeg Department of Anatomy, Yong Loo Lin School Medicine, National University Singapore, Singapore 117594, Pediatrics, New York Medical College, Valhalla, NY 10595, USA Division Pathology, General Hospital, 169856, Academic Clinical Program for Duke-NUS Graduate School, 169857, Correspondence to: Baeg, email: antbgh@nus.edu.sg Keywords: JAK/STAT signaling; GRAMD1B;...
Zinc oxide nanoparticles (ZnO NPs) have a wide range of applications, but the number reports on ZnO NP-associated toxicity has grown rapidly in recent years. However, studies that elucidate underlying mechanisms for NP-induced are scanty. We determined profiles NPs using both vitro and vivo experimental models. A significant decrease cell viability was observed NP-exposed MRC5 lung fibroblasts, showing exert cytotoxic effects. Similarly, interestingly, gut exposed to exhibited dramatic...
Abstract Gain‐ or loss‐of‐function mutations in Janus kinase 3 (JAK3) contribute to the pathogenesis of various haematopoietic malignancies and immune disorders, suggesting that aberrant JAK3 signalling is an attractive therapeutic target treat these disorders. In this study, we performed structure‐based computational database screening using 3D structure domain National Cancer Institute diversity set identified tubulosine as a novel inhibitor. Tubulosine directly blocked catalytic activity...
Maintenance of stem cells requires the concerted actions niche-derived signals and cell-intrinsic factors. Although Decapentaplegic (Dpp), a Drosophila bone morphogenetic protein (BMP) molecule, can act as long-range morphogen, its function is spatially limited to germline cell niche in germarium. We show here that Integrator, complex known be involved RNA polymerase II (RNAPII)-mediated transcriptional regulation nucleus, promotes differentiation by restricting Dpp/BMP activity cytoplasm....