The amount of the 64-kDa subunit polyadenylation/cleavage stimulatory factor (CstF-64) increases 5-fold during G 0 to S phase transition and concomitant proliferation induced by serum in 3T6 fibroblasts. Higher levels CstF-64 result an increase CstF trimer. rise occurs at a time when poly(A)-containing RNA rose least 5–8 fold cytoplasm. Primary human splenic B cells, resting , show similar cultured under conditions inducing (CD40 ligand exposure). Therefore, is associated with transition. As...

Prion diseases are caused by conformational alterations in the prion protein (PrP). The immune system has been assumed to be non-responsive self-prion protein, therefore, PrP autoimmunity not investigated. Here, we immunized various strains of mice with peptides, some selected fit MHC class II-peptide binding motif. We found that specific peptides elicited strong responses NOD, C57BL/6 and A/J mice. To test functional effect this immunization, examined expression proteinase-K-resistant a...

Seroprevalence studies of SARS-CoV-2 antibodies are useful in assessing the epidemiological status community, and degree spread.To study seroprevalence rates among healthy blood donors Jordan, at various points time as pandemic evolves community.In total, 1374 donor samples, from three groups, were tested for total immunoglobulin antibodies. In first group, samples 734 individuals (from donations made between January June 2020) June. second 348 September 2020. The third group 292 was...

Background: Cytomegalovirus (CMV) infection can lead to significant morbidity and mortality in pediatric hematopoietic stem cell transplant recipients. Early detection of CMV is crucial for managing its impact. Aim: This study aims evaluate the effectiveness QuantiFERON-CMV ® (QF-CMV) QuantiFERON-Monitor (QFM) tests predicting graft-versus-host disease (GvHD) recipients enhance patient outcomes support personalized prevention strategies. Methods: The QF-CMV QFM were used predict pp65 antigen...

Abstract Background Covid-19 seroprevalence rates and serological tests are important tools in understanding the epidemiology of disease help fight against it. Seroprevalence vary according to population studied test employed they range from 0.133 25.7%. The purpose this study is assess rate a healthy blood donors living under strict lockdown measures Jordan which has total 144 confirmed cases per million population. Methods Left-over sera plasma samples 746 were tested using commercially...

Abstract We recently reported that immunization of mice with certain self‐prion protein peptides induced specific T‐cell and B‐cell immune responses; importantly, this was associated a decrease in the number protease‐resistant PrP Sc particles recoverable transplanted, scrapie‐infected syngeneic tumor. The present study carried out to determine whether immunogenic might influence natural history experimental scrapie mice. immunized C57BL/6 complete Freund's adjuvant (CFA) or CFA alone as...

Introduction: Statistics on the prevalence of donor screening for hepatitis B surface antigen (HBsAg) and anti-hepatitis C virus (anti-HCV) in Jordan are outdated. There no studies anti-HIV I/II, anti-human T-cell lymphotropic type I II (anti-HTLV-I/II), or anti-syphilis. Data also lacking significance using anti-HBc Jordan. This study aimed to evaluate transfusion-transmissible infections (TTI) among donors at King Hussein Cancer Center compare it with neighboring countries anti-HB core...

Background: Aside from the pandemic's negative health effects, world was confronted with public confusion since proper communication and favorable decisions became an ongoing challenge. As a result, public's perceptions were influenced by what they knew, many sources of COVID-19 information, how interpreted it. With cancer patients continuing to oppose vaccines, we sought investigate pandemic vaccine this information in adult patients, which either helped or prevented them taking vaccine. We...

Research studies aimed at advancing cancer prevention, diagnosis, and treatment depend on a number of key resources, including ready supply high-quality annotated biospecimens from diverse ethnic populations that can be used to test new drugs, assess the validity prognostic biomarkers, develop tailor-made therapies. In November 2011, KHCCBIO was established King Hussein Cancer Center (KHCC) with support Seventh Framework Programme (FP7) funding European Union (khccbio.khcc.jo). developed for...

Abstract Cytomegalovirus (CMV) is the most prevalent infection in recipients of hematopoietic stem cell transplant (HSCT). QuantiFERON‐CMV (QF‐CMV) and QuantiFERON‐Monitor (QFM) assays were used to test whether immune‐competent adult allogeneic HSCT with CMV‐specific T cells can control CMV or reactivation. Our data demonstrated a significant correlation between measured by CMV‐antigenemia QF‐CMV results, graft versus host disease (GvHD), mortality rates. The revealed that higher...

Abstract Background The coronavirus disease 2019 (COVID-19) pandemic has resulted in more than 106 million cases of confirmed infection and 2.3 deaths worldwide as February 11th 2021. Seroprevalence studies are extremely useful studying assessing the epidemiological status community degree spread. They help decision makers implementing or relaxing mitigating measures to contain addition other benefits. Objective To study seroprevalence rates SARS-CoV-2 antibodies among healthy blood donors...

Abstract Background Hepatitis B and C infections transmission are a serious challenge to all healthcare systems. We studied seroprevalence rates of Transfusion Transmitted Diseases (TTD) among blood bank donors in Jordan from 2014 2019 as follow-up study our previously published work. In addition, we wanted explore the efficacy mandatory vaccination infants against hepatitis virus (HBV) which was implemented by Ministry Health since 1995 for eradication HBV infection Jordan. Methods reviewed...

Background: Dexamethasone is used to treat cancer, relieve chemotherapy-induced nausea and vomiting, enhance cancer patients’ appetites, COVID-19 patients. There little evidence of the impact a dexamethasone treatment plan on severity infections in This study explores whether influences dexamethasone-treated Methods: The medical records 108 patients receiving at King Hussein Cancer Center with infection 343 without corticosteroid were reviewed. Patients within seven days before infection,...

Objective: The kinetics of immune responses to various SARS-CoV-2 vaccines in cancer patients were investigated. Methods: In total, 57 who received BNT162b2-RNA or BBIBP-CorV enrolled. Cellular and humoral immunity assessed at three-time points, before the first vaccine dose 14–21 days after second doses. Chemiluminescent microparticle immunoassay was used evaluate anti-spike IgG response, QuantiFERON® kit T-cell response. Results: Data showed that patients’ CD4+ CD8+ T cell-median IFN-γ...

Background: The effective immunization of healthcare workers (HCWs) plays a vital role in preventing the spread SARS-CoV-2 infection during coronavirus disease 2019 (COVID-19) pandemic. There is limited data on immune response to vaccination among HCWs. We aim determine seroprevalence rates and neutralizing IgG antibody various immunizations Methods: This study was conducted between July September 2021, which blood samples were obtained from HCWs antibodies measured. Data regarding status...

This paper presents the design and development of a new Quality Control (QC) system for immunology/serology tests at medical laboratories. The developed QC which is based on client-server architecture provides scalable future integration other categories laboratory tests. functional features proposed are presented along with workflow process UML models key management processes. More than 15 graphical user interface (GUI) forms designed implemented to enter/display various types information....

Anti-inflammatory corticosteroids are used in cancer treatment and COVID-19 infections. Data on the impact of non-dexamethasone infection severity patients minimal. This study investigates if corticosteroid affects disease adult patients.A total 116 COVID-19-infected hydrocortisone (H) or prednisone (P) were compared to 343 untreated patients. The included who received before (B), after (A), both (B A) Ventilation support, hospitalization mortality investigated.Our data showed that a...

The effective development of COVID-19 vaccination has mitigated its harm. Using two laboratory methods, we investigated the efficacy BNT162b2 mRNA and BBIBP-CorV vaccines on seroconversion rates in cancer patients undergoing active treatment.SARS-CoV-2 were scheduled for 134 individuals. consenting participants submitted three venous blood samples. Three samples: T0, T1, T2. ABBOTT-SARS-CoV-2 IgG II Quant Elecsys® Anti-SARS-CoV-2 assays used to evaluate samples convert antibody titers WHO...