A5075692086- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Cytomegalovirus and herpesvirus research
- Long-Term Effects of COVID-19
- HIV-related health complications and treatments
- COVID-19 and Mental Health
- COVID-19 epidemiological studies
- T-cell and B-cell Immunology
- SARS-CoV-2 detection and testing
- Herpesvirus Infections and Treatments
- Immunodeficiency and Autoimmune Disorders
- Hepatitis C virus research
- Respiratory viral infections research
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Viral-associated cancers and disorders
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Vagus Nerve Stimulation Research
- COVID-19 and healthcare impacts
IrsiCaixa
2013-2025
Centro de Investigación Biomédica en Red
2021-2025
Instituto de Salud Carlos III
2022-2025
Hospital Universitari Germans Trias i Pujol
2010-2024
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2011-2024
Universitat de Vic - Universitat Central de Catalunya
2022-2023
Centre Hospitalier de l’Université de Montréal
2016-2023
Fundació ACE
2023
Universitat Autònoma de Barcelona
2010-2022
Université de Montréal
2015-2022
The phenotypic characterization of the cells in which HIV persists during antiretroviral therapy (ART) remains technically challenging. We developed a simple flow cytometry-based assay to quantify and characterize infected producing proteins untreated treated infection. By combining two antibodies targeting capsid standard intracellular staining protocol, we demonstrate that p24-producing can be detected with high specificity sensitivity blood from people living HIV. In individuals,...
Abstract The protective effect of neutralizing antibodies in SARS-CoV-2 infected individuals is not yet well defined. To address this issue, we have analyzed the kinetics antibody responses and their association with disease severity. Between March May 2020, prospective KING study enrolled 72 COVID-19+ participants grouped according to infection was diagnosed by serological virological tests. Plasma were assessed against replicative virus pseudoviral particles. Multiple regression...
Inappropriate sinus tachycardia (IST) is a common observation in patients with post-COVID-19 syndrome (PCS) but has not yet been fully described to date. To investigate the prevalence and mechanisms underlying IST prospective population of PCS patients. Consecutive admitted Unit between June December 2020 resting rhythm rate ≥ 100 bpm were prospectively enrolled this study further examined by an orthostatic test, 2D echocardiography, 24-h ECG monitoring (heart variability was surrogate for...
At least 5-10% of subjects surviving COVID-19 develop the post-COVID-19 condition (PCC) or "Long COVID". The clinical presentation PCC is heterogeneous, its pathogenesis being deciphered, and objective, validated biomarkers are lacking. It unknown if a single entity heterogeneous syndrome with overlapping pathophysiological basis. large US RECOVER study identified four clusters according to their presenting symptoms. However, long-term implications remain unknown.We conducted 2-year...
High levels of HIV-1 replication during the chronic phase infection usually correlate with rapid progression to severe immunodeficiency. However, a minority highly viremic individuals remains asymptomatic and maintains high CD4+ T cell counts. This tolerant profile is poorly understood reminiscent widely studied nonprogressive disease model SIV in natural hosts. Here, we identify transcriptome differences between progressors (RPs) nonprogressors (VNPs) highlight several genes relevant for...
Although antiretroviral therapy improves immune response, some human immunodeficiency virus-infected patients present unsatisfactory CD4 T cell recovery despite achieving viral suppression, resulting in increased morbidity and mortality.Cross-sectional, case-control study to characterize the mechanism identify predictive factors of poor response. We included 230 who were receiving highly active had a load <50 copies/mL for >2 years; 95 "discordant" (case patients; count always <350...
The failure to increase CD4 T-cell counts in some HAART-treated HIV-infected patients with satisfactory virological responses has been related low production, high turnover and death. However, the relative contribution of these factors is still unclear, strongly limiting definition appropriate therapeutic strategies for patients.A cross-sectional study was designed evaluate thymic activity, microbial translocation, cellular activation death recovery. We included 230 individuals on...
Background Residual viraemia is a major obstacle to HIV-1 eradication in subjects receiving HAART. The intensification with raltegravir could impact latent reservoirs and might lead reduction of plasma (viral load [VL]), complementary DNA intermediates immune activation. Methods This was prospective, open-label, randomized study comprising 69 individuals on suppressive HAART randomly assigned 2:1 add during 48 weeks. Results Total integrated DNA, ultrasensitive VL remained stable despite...
Objective: Early HIV infection is characterized by a dramatic depletion of CD4 T cells in the gastrointestinal tract and translocation bacterial products from gut into blood. In this study, we evaluated if profiles were associated with immune status before after starting antiretroviral therapy (ART). Design: We microbiota men recently infected (n = 13) who participating randomized, double-blind controlled trial combination ART maraviroc versus placebo followed for 48 weeks. Methods: To...
The phenotype of the rare HIV-infected cells persisting during antiretroviral therapies (ART) remains elusive. We developed a single-cell approach that combines phenotypic analysis with near full-length sequencing their associated proviruses to characterize viral reservoir in 6 male individuals on suppressive ART. show individual carrying clonally expanded identical display very diverse phenotypes, indicating cellular proliferation contributes diversification HIV reservoir. Unlike most...
Background The COVID-19 pandemic’s long-term mental health implications are increasingly concerning, especially among patients suffering post-acute sequelae of SARS-CoV-2 infection: Long COVID (LC) patients. Aim This study explores the presence and distribution anxiety, depression, stress in LC individuals with cognitive complaints northern Barcelona (Spain). Design & settings cross-sectional involved 155 diagnosed from “Aliança ProHEpiC-19 Cognitiu (APC)” project. Method Demographic...
Abstract Background Chronic Fatigue Syndrome (CFS) is a debilitating neuro-immune disorder of unknown etiology diagnosed by an array clinical manifestations. Although several immunological abnormalities have been described in CFS, their heterogeneity has limited diagnostic applicability. Methods Immunological features CFS were screened 22 individuals fulfilling Fukuda criteria and 30 control healthy individuals. Peripheral blood T, B NK cell function phenotype analyzed flow cytometry both...
Abstract Multiple scientific disciplines require the isolation of specific subsets blood cells from patient samples for gene expression analysis by microarray or RNA‐sequencing, preserving disease‐ treatment‐related signatures. However, little is known with respect to impact different cell methods on and effects positive selection, negative fluorescence activated sorting (FACS) have not previously been assessed in parallel. To address this knowledge gap, CD4+ T cells, CD8+ B monocytes were...
Asymptomatic cytomegalovirus (CMV) replication occurs frequently in the genital tract untreated HIV-infected men and is associated with increased immune activation HIV disease progression. To determine connections between CMV-associated size of viral reservoir, we evaluated interactions (i) asymptomatic seminal CMV replication, (ii) levels T cell proliferation blood, (iii) transcriptional activity DNA reservoir blood from 53 on long-term antiretroviral therapy (ART) suppressed RNA plasma. We...
ABSTRACT Asymptomatic replication of human herpesviruses (HHV) is frequent in HIV-infected men and associated with increased T-cell activation HIV disease progression. We hypothesized that the presence cytomegalovirus (CMV) Epstein-Barr virus (EBV) (the most frequently detected HHV) might influence DNA decay during antiretroviral therapy (ART). investigated 607 peripheral blood mononuclear cell (PBMC) samples from 107 CMV-seropositive, who have sex men, started ART within a median 3 months...
BackgroundChronic inflammation and residual HIV transcription persist in people living with (PLWH) receiving antiretroviral therapy (ART), thus increasing the risk of developing non-AIDS co-morbidities. The mechanistic target rapamycin (mTOR) is a key regulator cellular metabolism transcription, therefore represents an interesting novel therapeutic target.MethodsThe LILAC pilot clinical trial, performed on non-diabetic ART-treated PLWH CD4+/CD8+ T-cell ratios <0.8, evaluated effects...
BackgroundEarly antiretroviral therapy (ART) initiation (ie, within 3 months of infection) limits establishment the HIV reservoir. However, effect early ART on long-term dynamics pool infected cells remains unclear.MethodsIn this longitudinal analysis, we included cisgender men who have sex with (MSM) and transgender women (aged 18–54 years) at high risk for infection, enrolled in ongoing MERLIN study Peru between Oct 28, 2014, Nov 8, 2018. Participants were eligible if they had been less...