A5015130845- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Molecular Biology Techniques and Applications
- Glycosylation and Glycoproteins Research
- Mass Spectrometry Techniques and Applications
- Advanced NMR Techniques and Applications
- Glycogen Storage Diseases and Myoclonus
- Advanced Proteomics Techniques and Applications
- Mitochondrial Function and Pathology
- Signaling Pathways in Disease
- Mechanical Circulatory Support Devices
- Cardiac Arrhythmias and Treatments
- MicroRNA in disease regulation
- Amino Acid Enzymes and Metabolism
- Diet and metabolism studies
- Cardiac pacing and defibrillation studies
- Genetics and Neurodevelopmental Disorders
- Alzheimer's disease research and treatments
- Cancer-related Molecular Pathways
- Neuroendocrine Tumor Research Advances
University of Kentucky
2021-2025
Markey Cancer Center
2022
Brain glucose metabolism is highly heterogeneous among brain regions and continues postmortem. In particular, we demonstrate exhaustion of glycogen an increase in lactate production during conventional rapid resection preservation by liquid nitrogen. contrast, show that these postmortem changes are not observed with simultaneous animal sacrifice situ fixation focused, high-power microwave. We further employ microwave to define the mouse model streptozotocin-induced type 1 diabetes. Using...
MYCN-amplification is a genetic hallmark of ~40% high-risk neuroblastomas (NBs). Altered glycosylation common feature adult cancer progression, but little known about how signatures such as alter profiles. Herein, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased core fucosylated glycan abundance within neuroblast-rich regions human MYCN-amplified NB tumors. GDP-mannose 4,6-dehydratase (GMDS) responsible for the first-committed and...
Abstract Introduction: MYCN-amplification (MYCN-amp) is a genetic hallmark of ~40% high-risk neuroblastomas (NBs). N-MYC an oncogenic transcription factor and master regulator metabolism. Glycosylation major post-translational modification that critical for cancer progression, spread, immune evasion. How signatures such as MYCN-amp alter glycosylation profiles unknown. Herein, we utilized matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to define the N-linked...
<title>Abstract</title> <italic>MYCN-</italic>amplification is a genetic hallmark of ~ 40% high-risk neuroblastomas (NBs). Altered glycosylation common feature adult cancer progression, but little known about how signatures such as <italic>MYCN</italic>-amplification alter profiles. Herein, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased core fucosylated glycan abundance within human <italic>MYCN-</italic>amplified NB tumors. GDP-mannose...
ABSTRACT The brain metabolome directly connects to physiology and neuronal function. Brain glucose metabolism is highly heterogeneous among regions continues postmortem. Therefore, challenges remain capture an accurate snapshot of the physiological in healthy diseased rodent models. To overcome this barrier, we employ a high-power focused microwave for simultaneous euthanasia fixation mouse tissue preserve metabolite pools prior surgical removal dissection regions. We demonstrate exhaustion...
Lung adenocarcinoma (LUAD) is the major histological subgroup of non-small cell lung cancer (NSCLC) with an extremely high mortality rate even when diagnosed at early stage. LUAD makes up around 40% cases and can quickly metastasize to other areas body. Glycogen short-term storage carbohydrates be easily mobilized in Recently, our own data suggests glycogen drive progression, however, mechanism which accumulates poorly understood potentially arises from a number genetic environmental...
Lung cancer is one of the most prevalent cancers worldwide, accounting for nearly two million new cases each year. adenocarcinoma (LUAD) common subtype lung and leading cause related mortalities worldwide. LUAD has an extremely poor five-year survival rate, which likely due to being diagnosed at advanced stages thus more difficult treat metastatic disease. Identifying molecular processes that contribute disease progression a critical goal in research could lead predictive biomarkers novel...
Lung cancer is the leading cause of related death in United States, with patients from rural Appalachia having drastically higher incidence and mortality rates, driven by largely unknown mechanisms. Identifying molecular features that contribute to this health disparity a critical step lung research could lead predictive biomarkers personalized therapy for population. Recently, aberrant glycogen accumulation tumors has been reported promote progression. However, full clinical implications...
Alzheimer's disease (AD) is an incurable dementia known for its neurodegenerative pathologies including amyloid beta plaques, hyperphosphorylated neurofibrillary tau tangles, and brain glucose hypometabolism. One key metabolic pathway that also utilizes glycan synthesis, which to be disrupted in AD. Glycosylation essential protein modification requires flux through the hexosamine pathway. Prior research our lab has demonstrated glycosylation abnormalities can detected peripheral blood of...
Abstract Lung cancer is the leading cause of related death in United States, with patients from rural Appalachia having drastically higher incidence and mortality rates, driven by largely unknown mechanisms. Identifying molecular features that contribute to this health disparity a critical step lung research could lead predictive biomarkers personalized therapy for population. Recently, aberrant glycogen accumulation tumors has been reported promote progression. However, full clinical...
Abstract Lung cancer is one of the most prevalent cancers worldwide, accounting for nearly two million new cases each year. adenocarcinoma (LUAD) common subtype lung and leading cause related mortalities worldwide. LUAD has an extremely poor five-year survival rate, which likely due to being diagnosed at advanced stages thus more difficult treat metastatic disease. Identifying molecular processes that contribute disease progression a critical goal in research could lead predictive biomarkers...
Abstract Lung cancer is the leading cause of related death worldwide, with a high mortality rate even when diagnosed at an early stage. Identifying unique molecular features that drive disease progression within distinct tumor subtypes critical step in lung research could advance our understanding biology, to predictive biomarkers and novel therapies. Glycogen primary source carbohydrate storage most issues, its degradation products are intimately connection central carbon metabolism....