A5008989724- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Diabetes Management and Research
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- RNA modifications and cancer
- Pancreatic function and diabetes
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Cancer-related Molecular Pathways
- Diabetes Treatment and Management
- Glycogen Storage Diseases and Myoclonus
- Cancer Research and Treatments
- Liver Disease Diagnosis and Treatment
- Immune cells in cancer
- Diet, Metabolism, and Disease
- Cancer, Lipids, and Metabolism
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Prostate Cancer Treatment and Research
- Amino Acid Enzymes and Metabolism
- Lung Cancer Research Studies
- Diabetes and associated disorders
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
University of Kentucky
2020-2025
Insulet (United States)
2024
Markey Cancer Center
2021-2023
University of Florida
2023
Nia Association
2022
ID Genomics (United States)
2022
University of Louisville
2020
Macrophages use mitochondrial lactate metabolism for ACLY-dependent histone acetylation, immune suppression, and tumor progression.
Background: The Omnipod® 5 Automated Insulin Delivery System was associated with favorable glycemic outcomes for people type 1 diabetes (T1D) in two pivotal clinical trials. Real-world evidence is needed to explore effectiveness nonstudy conditions. Methods: A retrospective analysis of the United States Omnipod users (aged ≥2 years) T1D and sufficient data (≥90 days data; ≥75% ≥220 continuous glucose monitor readings/day) available Insulet Corporation's device person-reported datasets as...
Importance There is a need for additional treatment options people with type 2 diabetes treated insulin. Given the limited data on use of automated insulin delivery (AID) systems in diabetes, studies evaluating their safety and efficacy are important. Objective To evaluate association AID hemoglobin A 1c (HbA ) levels diverse cohort adults diabetes. Design, Setting, Participants This single-arm prospective trial was conducted at 21 clinical centers United States among individuals aged 18 to...
Abstract Matrix assisted laser desorption/ionization imaging has greatly improved our understanding of spatial biology, however a robust bioinformatic pipeline for data analysis is lacking. Here, we demonstrate the application high-dimensionality reduction/spatial clustering and histopathological annotation matrix datasets to assess tissue metabolic heterogeneity in human lung diseases. Using features identified from this pipeline, hypothesize that channeling between glycogen N-linked...
N-linked protein glycosylation in the brain is an understudied facet of glucose utilization that impacts a myriad cellular processes including resting membrane potential, axon firing, and synaptic vesicle trafficking. Currently, spatial map glycans within normal Alzheimer's disease (AD) human does not exist. A comprehensive analysis glycome would improve our understanding energy metabolism, linking metabolism to signaling events perturbed during AD progression, could illuminate new...
N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution tissues; therefore, spatial analyses of these crucial to our understanding physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique enables situ detection analytes with distribution. This workflow details a MALDI-MSI protocol for the profiling from tissues following application enzyme MALDI...
Abstract Prostate cancer is the most common in men worldwide. Despite its prevalence, there a critical knowledge gap understanding factors driving disparities survival among different cohorts of patients with prostate cancer. Identifying molecular features separating disparate populations an important first step research that could lead to fundamental hypotheses biology, predictive biomarker discovery, and personalized therapy. N-linked glycosylation cotranslational event during protein...
Brain glucose metabolism is highly heterogeneous among brain regions and continues postmortem. In particular, we demonstrate exhaustion of glycogen an increase in lactate production during conventional rapid resection preservation by liquid nitrogen. contrast, show that these postmortem changes are not observed with simultaneous animal sacrifice situ fixation focused, high-power microwave. We further employ microwave to define the mouse model streptozotocin-induced type 1 diabetes. Using...
Definitive hematopoietic stem and progenitor cells (HSPCs) development relies on intrinsic extrinsic programs to meet homeostatic stress-related demands. The comprehensive mechanisms governing HSPCs fate remain poorly understood. Our study identifies B4GALT1, a glycosyltransferase essential for N-glycosylation, as key modulator of HSPC lineage decisions. We demonstrate that B4GALT1 deficiency disrupts glycosylation patterns within the bone marrow (BM) niche, resulting in oncogenic glycan...
Excessive fructose intake is a risk factor for the development of obesity and its complications. Targeting ketohexokinase (KHK), first enzyme metabolism, has been investigated management MASLD. We compared effects systemic, small molecule inhibitor KHK enzymatic activity to hepatocyte-specific, GalNAc-siRNA mediated knockdown in mice on HFD. measured activity, extensively quantified glycogen accumulation, performed RNAseq analysis, enumerated hepatic metabolites using mass spectrometry. Both...
Lafora disease is a rare and fatal progressive myoclonus epilepsy characterized by the accumulation of insoluble glycogen deposits in brain peripheral tissues. Mutations gene encoding phosphatase laforin result disease. Currently, there are no laforin-specific chemical probes, limiting our understanding roles metabolism other cellular processes. Here, we identified sulfophenyl acetic amide (SPAA), as novel nonhydrolyzable phosphotyrosine mimetic for inhibition. Using fragment-based...
Dysregulated metabolism is a hallmark of cancer cells and driven in part by specific genetic alterations various oncogenes or tumor suppressors. The retinoblastoma protein (pRb) suppressor that canonically regulates cell cycle progression; however, recent studies have highlighted functional role for pRb controlling cellular metabolism. Here, we report loss the gene encoding (Rb1) transgenic mutant Kras-driven model lung results metabolic reprogramming. Our tracer using bolus dosing...
Abstract Background Aberrant activity of cell cycle proteins is one the key somatic events in non-small lung cancer (NSCLC) pathogenesis. In most NSCLC cases, retinoblastoma protein tumor suppressor (RB) becomes inactivated via constitutive phosphorylation by cyclin dependent kinase (CDK) 4/6, leading to uncontrolled proliferation. Palbociclib, a small molecule inhibitor CDK4/6, has shown anti-tumor vitro and vivo, with recent studies demonstrating functional role for palbociclib...
Users new to the Omnipod 5 AID System can complete training and onboarding via personalized trainer-led education, virtually or in-person, for those upgrading from an earlier System, online, self-guided modules be completed. This study aimed evaluate outcomes by type among people with T1D initiating System. Continuous glucose monitoring (CGM) insulin data users in US aged ≥2y using ≥5 units of per day ≥90 days available cloud-based management system were included. Data 31,027 sufficient CGM...
Abstract Prostate cancer is the most common in men worldwide. Despite its prevalence, there a critical knowledge gap regarding underlining molecular events that result higher incidence and mortality rate Black men. Identifying features separate racial disparities step prostate research could lead to predictive biomarkers personalized therapy. N-linked glycosylation co-translational event during protein folding modulates myriad of cellular processes. Recently, aberrant has been reported...
The Omnipod 5 AID System was associated with improvement in glycemic outcomes for people T1D regardless of baseline management two single-arm pivotal clinical trials. Baseline HbA1c is an important factor to consider when optimizing AID, as new user needs and expectations vary. This study evaluated real-world the users sub-optimal >9% prior starting on system. CGM insulin data from aged ≥2y using ≥5 units per day US ≥90 days available cloud-based system were included. Data 7,876...
Abstract Aim Automated insulin delivery (AID) systems have demonstrated improved glycaemic outcomes in people with type 1 diabetes (T1D), yet limited data exist on these very young children and their impact caregivers. We evaluated psychosocial following use of the tubeless Omnipod® 5 AID System caregivers children. Materials Methods This 3‐month single‐arm, multicentre, pivotal clinical trial enrolled 80 aged 2.0–5.9 years T1D to Omnipod System. Caregivers completed questionnaires assessing...
ABSTRACT The brain metabolome directly connects to physiology and neuronal function. Brain glucose metabolism is highly heterogeneous among regions continues postmortem. Therefore, challenges remain capture an accurate snapshot of the physiological in healthy diseased rodent models. To overcome this barrier, we employ a high-power focused microwave for simultaneous euthanasia fixation mouse tissue preserve metabolite pools prior surgical removal dissection regions. We demonstrate exhaustion...