A5043660083- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Birth, Development, and Health
- Pancreatic function and diabetes
- Gestational Diabetes Research and Management
- Metabolism, Diabetes, and Cancer
- Growth Hormone and Insulin-like Growth Factors
- Regulation of Appetite and Obesity
- Diabetes and associated disorders
- Liver Disease Diagnosis and Treatment
- Protein Kinase Regulation and GTPase Signaling
- Peroxisome Proliferator-Activated Receptors
- Exercise and Physiological Responses
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and biosynthesis
- Pregnancy and preeclampsia studies
- Fatty Acid Research and Health
- Sirtuins and Resveratrol in Medicine
- Signaling Pathways in Disease
- Angiogenesis and VEGF in Cancer
- Neonatal Health and Biochemistry
- Antiplatelet Therapy and Cardiovascular Diseases
- Lipid metabolism and disorders
- Cytokine Signaling Pathways and Interactions
- Blood Coagulation and Thrombosis Mechanisms
University of California, San Diego
2013-2024
Shandong University
2007-2023
Jinan Central Hospital
2016-2017
Palmetto Hematology Oncology
2017
Shandong Provincial Hospital
2007-2012
UC San Diego Health System
2012
University of Kentucky
2005-2010
University of Colorado Denver
2007
University of Colorado Health
2003-2005
Case Western Reserve University
1999-2005
Adiponectin is an adipose-derived hormone that plays important role in maintaining energy homeostasis. gene expression diminished both obesity and type 2 diabetes. However, the mechanism underlying impaired adiponectin remains poorly understood. Recent studies have indicated forkhead transcription factor O1 (Foxo1) silent information regulator mammalian ortholog SIRT1 are involved adipogenesis. Here we shown Foxo1 up-regulates through a Foxo1-responsive region mouse promoter contains two...
Women who develop gestational diabetes mellitus (GDM) have severe insulin resistance and markedly increased risk to subsequent type 2 diabetes. We investigated the effects of pregnancy GDM on glucose transport activity expression phosphorylation receptor substrate (IRS)-1 in human skeletal muscle fiber strips vitro. Rectus abdominis biopsies were obtained at time cesarean section from 11 pregnant women with normal tolerance (pregnant control), 7 GDM, nonpregnant undergoing elective surgery...
Gestational diabetes mellitus (GDM) is associated with elevated postprandial free fatty acids (FFA) and insulin resistance; however, little known about the cellular mechanisms underlying resistance to suppress lipolysis during gestation. We evaluated longitudinal changes in suppression of FFA before pregnancy early (12–14 wk) late (34–36 gestation obese subjects normal glucose tolerance GDM subjects. Abdominal subcutaneous adipose tissue biopsies were also obtained cesarean delivery from...
Abstract The insulin resistance of normal pregnancy is necessary to divert fuels the fetus meet fetal growth demands and mediated by placental hormones. We recently demonstrated that human GH (hPGH) can trigger severe in transgenic (TG) mice. In this study we sought elucidate cellular mechanisms which hPGH interferes with signaling muscle TG Insulin-stimulated GLUT-4 translocation plasma membrane (PM) was reduced compared wild-type (WT) mice (P = 0.05). Insulin receptor (IR) levels were...
OBJECTIVE—Adiponectin is an adipocyte-derived hormone that plays important role in glucose and lipid metabolism. The main aims of this study are to investigate the effects adiponectin on VLDL triglyceride (VLDL-TG) metabolism underlying mechanism. RESEARCH DESIGN AND METHODS—Adenoviruses were used generate a mouse model with elevated circulating adiponectin. HepG2 C2C12 cells treated recombinant human RESULTS—Three days after Ad-mACRP30 adenovirus injection, plasma protein levels increased...
Adiponectin is an adipose-derived hormone that enhances insulin sensitivity and plays important role in regulating energy homeostasis. Here, we demonstrate the DNA encoding first intron of human adiponectin gene contains intronic enhancer regulates expression adipose tissue–specific manner. Insertion into reporter constructs containing proximal promoter (Pro-Int1-Luc) resulted a 20-fold increase activity relative to alone 3T3-L1 adipocytes. Coexpression CCAAT/enhancer-binding protein...
Recent studies suggest that the serine/threonine kinase protein B (PKB or Akt) is involved in pathway for insulin-stimulated glucose transporter 4 (GLUT4) translocation and uptake. In this study we examined components of Akt signaling skeletal muscle adipose tissue vivo from C57BL/KsJ-Lepr(db/db) mice (db/db), a model obesity, insulin resistance, type II diabetes. There were no changes levels GLUT4, p85alpha, tissues db/db compared with non-diabetic littermate controls (+/+). response to...
CCAAT/enhancer-binding protein β (C/EBPβ) plays a key role in initiation of adipogenesis adipose tissue and gluconeogenesis liver; however, the C/EBPβ hepatic lipogenesis remains undefined. Here we show that inactivation Leprdb/db mice attenuates obesity, fatty liver, diabetes. In addition to impaired adipogenesis, livers from C/EBPβ-/- x had dramatically decreased triglyceride content reduced lipogenic enzyme activity. deletion down-regulated peroxisome proliferator-activated receptor γ2...
Adiponectin is an adipocyte-derived hormone that sensitizes insulin and improves energy metabolism in tissues. This study was designed to investigate the direct regulatory effects of adiponectin on lipid adipocytes.Basal hormone-stimulated lipolysis were comparatively analyzed using white adipose tissues or primary adipocytes from gene knockout control mice. To further underlying mechanisms through which suppresses lipolysis, cultured 3T3-L1 adenovirus-mediated transduction...
Adiponectin enhances mitochondrial biogenesis and oxidative metabolism in skeletal muscle. This study aimed to investigate the underlying mechanisms through which adiponectin induces Mitochondrial contents, expression, activation status of p38 mitogen-activated protein kinase (MAPK) PPARγ coactivator 1α (PGC-1α) were compared between muscle samples from gene knockout, adiponectin-reconstituted, control mice. Adenovirus-mediated MAPK phosphatase-1 (MKP1) overexpression used verify...
Hypoadiponectinemia has been widely observed in patients with gestational diabetes mellitus (GDM). To investigate the causal role of hypoadiponectinemia GDM, adiponectin gene knockout (
The cellular mechanisms for the insulin resistance of pregnancy and gestational diabetes mellitus (GDM) are unknown. membrane protein plasma cell glycoprotein-1 (PC-1) has been identified as an inhibitor receptor tyrosine kinase (IRTK) activity. We investigated function PC-1 levels in muscle from three groups obese subjects: women with GDM, pregnant normal glucose tolerance, nonpregnant control subjects. Subjects (n = 6 each group) were similar age degree obesity (body fat >30%). IRTK...
Gestational diabetes mellitus (GDM) results from an interaction between susceptibility genes and the diabetogenic effects of pregnancy. During pregnancy, mice heterozygous for lepin receptor (db/+) gain more weight, are glucose intolerant, produce macrosomic fetuses compared with wild-type (+/+) mothers, suggesting that alteration in leptin action may play a role GDM fetal overgrowth. To investigate whether administration or pair-feeding can reduce adiposity thereby prevent neonatal...
The restoration of maternal insulin sensitivity postpartum represents an important physiological and metabolic adaptation in a woman's reproductive lifespan. present study was conducted to examine the potential cellular mechanisms underlying changes from late pregnancy human skeletal muscle. Nine nonobese women (age, 32 ± 2 yr; body mass index, 21.2 0.8 kg/m2) with normal glucose tolerance were studied during (30–36 wk) again approximately 1 yr using euglycemic-hyperinsulinemic clamp (5 mm...
This study investigated how maternal overnutrition and obesity regulate expression activation of proteins that facilitate lipid transport in the placenta. To create a model, primiparous C57BL/6 mice were fed high-fat (HF) diet throughout gestation. Fetuses from HF-fed dams had significantly increased serum levels free fatty acid body fat. Despite no significant difference placental weight, lipoprotein lipase (LPL) protein activity remarkably elevated placentas dams. Increased triglyceride...
Porphyromonas gingivalis, a major periodontal pathogen, invades autophagosomes of cells, including gingival epithelial endothelial fibroblasts, macrophages, and dendritic to escape antimicrobial autophagy lysosome fusion. However, it is not known how P. gingivalis resists autophagic immunity, survives within induces inflammation. Thus, we investigated whether could by promoting efflux block maturation, leading intracellular survival, the growth cells results in cellular oxidative stress,...
Valproic acid (VPA) has been used for the treatment of epilepsy and bipolar disorders more than 30 yr. Obesity insulin resistance are common side effects VPA treatment. Adiponectin is an adipocyte-derived protein that plays important role in controlling sensitivity glucose homeostasis. In this report, we examined on adiponectin gene expression C57BL/6J mice differentiated 3T3-L1 adipocytes. significantly decreased mRNA levels both The adipocyte study showed inhibited a dose- time-dependent...
Acute hyperglycemia normally suppresses hepatic glucose production (HGP) and gluconeogenic gene expression. Conversely, chronic is accompanied by progressive increases in basal HGP a major contributor to both type 1 2 diabetes mechanisms that are poorly understood. The aim of this study was investigate the molecular whereby contributes excessive gluconeogenesis Fao hepatoma cells. Increasing from 5 20 mmol/l resulted loss inhibition PEPCK expression after 12 h. Furthermore, 24 h incubation...
Fatty acid translocase (FAT/CD36) plays an important role in facilitating long chain fatty transport. FAT/CD36 gene deletion protects mice from high fat diet-induced obesity. In this study we have investigated the regulatory mechanism of expression at transcription level. was activated during 3T3-L1 adipocyte differentiation, and protein levels were positively correlated with CCAAT/enhancer-binding alpha (C/EBPalpha) peroxisome proliferator-activated receptor gamma. However, a negative...
The molecular mechanism underlying adipogenesis and the physiological functions of adipose tissue are not fully understood. We describe here a unique mouse model severe lipodystrophy. Ablation Ptpn11/Shp2 in adipocytes, mediated by aP2-Cre , led to premature death, lack white fat, low blood pressure, compensatory erythrocytosis, hepatic steatosis Shp2 fat−/− mice. Fat transplantation partially rescued lifespan pressure mice, administration leptin also restored mutant animals with endogenous...
Hypoadiponectinemia and decreased adiponectin gene expression in white adipose tissue (WAT) have been well observed obese subjects animal models. However, the mechanism for obesity-associated hypoadiponectinemia is still largely unknown. To investigate regulatory role of energy intake, dietary fat, adiposity blood level, a series feeding regimens was employed to manipulate intake fat obese-prone C57BL/6, genetically ob/ob, obese-resistant A/J peroxisome proliferator-activated receptor-α...