A5057416821- Mesenchymal stem cell research
- Wound Healing and Treatments
- Pluripotent Stem Cells Research
- Protein Tyrosine Phosphatases
- Cytokine Signaling Pathways and Interactions
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- Hair Growth and Disorders
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Aortic aneurysm repair treatments
- Hedgehog Signaling Pathway Studies
- Electrospun Nanofibers in Biomedical Applications
- Neurogenesis and neuroplasticity mechanisms
- Mycobacterium research and diagnosis
- Fibroblast Growth Factor Research
- Dermatologic Treatments and Research
- interferon and immune responses
- Drug Transport and Resistance Mechanisms
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Tendon Structure and Treatment
- Aortic Disease and Treatment Approaches
Second Military Medical University
2001-2016
University of California, San Diego
2010-2015
Abstract Excessive scar formation caused by myofibroblast aggregations is of great clinical importance during skin wound healing. Studies have shown that mesenchymal stem cells (MSCs) can promote regeneration, but whether MSCs contribute to remains undefined. We found umbilical cord-derived (uMSCs) reduced and accumulation in a skin-defect mouse model. these functions were mainly dependent on uMSC-derived exosomes (uMSC-Exos) especially exosomal microRNAs. Through high-throughput RNA...
Long non-coding RNAs (lncRNAs) are known players in the regulatory circuitry of self-renewal human embryonic stem cells (hESCs). However, most hESC-specific lncRNAs remain uncharacterized. Here we demonstrate that growth-arrest-specific transcript 5 (GAS5), a tumour suppressor and growth arrest-related lncRNA, is highly expressed directly regulated by pluripotency factors OCT4 SOX2 hESCs. Phenotypic analysis shows GAS5 knockdown significantly impairs hESC self-renewal, but its overexpression...
The molecular mechanism underlying adipogenesis and the physiological functions of adipose tissue are not fully understood. We describe here a unique mouse model severe lipodystrophy. Ablation Ptpn11/Shp2 in adipocytes, mediated by aP2-Cre , led to premature death, lack white fat, low blood pressure, compensatory erythrocytosis, hepatic steatosis Shp2 fat−/− mice. Fat transplantation partially rescued lifespan pressure mice, administration leptin also restored mutant animals with endogenous...
The homing ability and secretory function of mesenchymal stem cells (MSCs) are key factors that influence cell involvement in wound repair. These controlled by multilayer regulatory circuitry, including adhesion molecules, core transcription (TFs) certain other regulators. However, the role molecules this circuitry their underlying mechanism remain undefined. In present paper, we demonstrate an molecule, junction molecule A (JAM-A), may as a promoter to regulate skin healing MSCs. vivo...
Early repair of skin injury and maximal restoration the function appearance have become important targets clinical treatment. In present study, we observed healing process defects in nude mice structural characteristics new after transplantation isolated cultured adipose derived mesenchymal stem cells (ADMSCs) onto human acellular amniotic membrane (AAM). The result showed that ADMSCs were closely attached to surface AAM grew well 24 h seeding. Comparison wound rate at days 7, 14, 28 seeded...
Significance Despite the extensive attentions paid to phosphatase and tensin homolog (Pten) or SH2-containing tyrosine (Shp2) functions in cell signaling, how their regulated pathways are intertwined has never been investigated. By creating a compound mutant mouse line with both genes deleted blood cells, we have found that Pten Shp2 can work antagonistically myelopoiesis, while acting cooperatively erythropoiesis. Consistently, pharmacological inhibition of suppressed myeloproliferative...
The dermal papilla cells in hair follicles function as critical regulators of growth. In particular, alopecia areata (AA) is closely related to the malfunctioning human (hDPCs). Thus, identifying regulatory mechanism hDPCs important inducing follicle (HF) regeneration AA patients. Recently, growing evidence has indicated that 3' untranslated regions (3' UTR) key genes may participate circuitry underlying cell differentiation and diseases through a so-called competing endogenous mechanism,...
Wound repair and functional reconstruction are two key aspects for treatment of skin injury. Research on cell source has become a focus study. The immune rejection induced by allograft cells the limited autologous epidermal stem have led to more attention multipotent adult progenitor (MAPC). In this study, we examined influence local environment injury migration differentiation MAPC in nude mice. homing wounds were investigated detecting expression specific antigens rat major...
Background: Recent evidence has suggested that p63 plays a particular role in maintaining the population of epidermal stem cells (ESCs), but expression pattern during normal rat epidermis development is still unknown. We investigated and differentiation identified whether specific marker ESCs cultured vitro . Methods: Skin biopsies were obtained from embryonic day (E) 12 embryo, newborn, 4‐week‐old rats. Immunohistochemistry was used to monitor profile p63. isolated newborn by rapid adhesion...
By establishing the indirect contact co-culture system, we studied in vitro condition for MAPCs differentiating into epidermal cells and transformation of cell phenotype. Cell culture insert membrane was used substitute basal MAPCs, fibroblast (FCs) mixture FCs were separately implanted 2 sides it. PKH26 to label cloned MAPCs; type IV collagen rapid adhering method isolate skin from 1-day-old SD rat. Part transformed expressing keratin presence peripheral epithelia FCs. Type successfully...