A5083668580- Digital Imaging for Blood Diseases
- Machine Learning in Healthcare
- AI in cancer detection
- Cancer, Hypoxia, and Metabolism
- Single-cell and spatial transcriptomics
- Angiogenesis and VEGF in Cancer
- Zebrafish Biomedical Research Applications
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Kruppel-like factors research
- Genetics and Neurodevelopmental Disorders
- Apelin-related biomedical research
- Epigenetics and DNA Methylation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Eicosanoids and Hypertension Pharmacology
- Supramolecular Self-Assembly in Materials
- Lanthanide and Transition Metal Complexes
- Advanced Fluorescence Microscopy Techniques
- Chemotherapy-induced organ toxicity mitigation
- Lipid metabolism and disorders
- Advanced Proteomics Techniques and Applications
- 3D Printing in Biomedical Research
- Cancer therapeutics and mechanisms
- Atherosclerosis and Cardiovascular Diseases
Massachusetts General Hospital
2017-2023
University of Alabama at Birmingham
2022
University of Chicago
2017-2022
Center for Systems Biology
2017
Harvard University
2017
The PD-1 pathway, consisting of the co-inhibitory receptor on T cells and its ligand (PD-L1) antigen-presenting (APCs), is a major mechanism tumor immune evasion. PD-L1 blockade antibodies have produced remarkable clinical activities against subset cancers. Binding between cell-intrinsic APC-intrinsic triggers inhibitory signaling to attenuate cell response. Here, we report that co-expressed with tumor-infiltrating APCs. Using reconstitution culture assays, demonstrate binds in cis. Such...
Biomechanical cues dynamically control major cellular processes, but whether genetic variants actively participate in mechanosensing mechanisms remains unexplored. Vascular homeostasis is tightly regulated by hemodynamics. Exposure to disturbed blood flow at arterial sites of branching and bifurcation causes constitutive activation vascular endothelium contributing atherosclerosis, the cause coronary artery disease (CAD) ischemic stroke (IS). Conversely, unidirectional promotes quiescent...
A T cell is a sensitive self-referential mechanical sensor. Mechanical forces influence the recognition, activation, differentiation, and function throughout lifetime of cell. cells constantly perceive respond to physical stimuli through their surface receptors, cytoskeleton, subcellular structures. Surface receptors receive cues in form generated receptor-ligand binding events, which are dynamically regulated by contact tension, shear stress, substrate rigidity. The resulting...
Vascular disease is a leading cause of morbidity and mortality in the United States globally. Pathological vascular remodeling, such as atherosclerosis stenosis, largely develop at arterial sites curvature, branching, bifurcation, where disturbed blood flow activates endothelium. Current pharmacological treatments complications principally target systemic risk factors. Improvements are needed. We previously devised targeted polyelectrolyte complex micelle to deliver therapeutic nucleotides...
BACKGROUND: Internal N7-methylguanosine (m7G) is a recently identified chemical modification of mammalian mRNA and component the epitranscriptome. While epitranscriptome plays key role in regulating RNA metabolism cellular function, specific contribution internal m7G to cardiovascular health disease remains unknown. Atherosclerosis preferentially develops at sites where disturbed blood flow activates endothelial cells, but whether its regulatory machinery influence mechanotransduction...
Little is understood about the embryonic development of sociality. We screened 1120 known drugs and found that inhibition topoisomerase IIα (Top2a) resulted in lasting social deficits zebrafish. In mice, prenatal Top2 caused defects interaction communication, which are behaviors relate to core symptoms autism. Mutation Top2a zebrafish down-regulation a set genes highly enriched for associated with autism humans. Both Top2a-regulated autism-associated gene sets have binding sites polycomb...
Hypoxia-inducible factors (HIFs) are the master regulators of angiogenesis, a process that is impaired in patients with diabetes mellitus (DM). The transcription factor aryl hydrocarbon receptor nuclear translocator (ARNT, also known as HIF1β) has been implicated development and progression diabetes. Angiogenesis driven primarily by endothelial cells (ECs), but both global EC-specific loss ARNT-cause associated embryonic lethality. Thus, we conducted experiments line mice carrying an...
Heterogeneous metabolism supports critical single-cell functions. Here, we describe deep-learning-enabled image analyses of a genetically encoded lactate-sensing probe which can accurately quantify metabolite levels and glycolytic rates at the level. Multiple strategies test data have been included to obviate possible obstacles including successful sensor expression accurate segmentation. This protocol reliably discriminates between metabolically diverse subpopulations be used directly link...
Abstract Biomechanical cues dynamically control major cellular processes but whether genetic variants actively participate in mechano-sensing mechanisms remains unexplored. Vascular homeostasis is tightly regulated by hemodynamics. Exposure to disturbed blood flow at arterial sites of branching and bifurcation causes constitutive activation vascular endothelium contributing atherosclerosis, the cause coronary artery disease (CAD) ischemic stroke (IS). Conversely, unidirectional promotes...
ABSTRACT Human infants exhibit innate social behaviors at birth, yet little is understood about the embryonic development of sociality. We screened 1120 known drugs and found that inhibition topoisomerase IIα (Top2a) resulted in lasting deficits zebrafish. In mice, prenatal Top2 caused behavioral defects related to core symptoms autism, including impairments interaction communication. Mutation Top2a zebrafish downregulation a set genes highly enriched for associated with autism humans. Both...
DISC1 is a genetic risk factor for multiple psychiatric disorders. Compared to the dozens of murine Disc1 models, there paucity zebrafish disc1 models-an organism amenable high-throughput experimentation. We conducted longitudinal neurobehavioral analysis mutant across key stages life. During early developmental stages, mutants exhibited abrogated behavioral responses sensory stimuli testing platforms. Moreover, during exposure an acoustic stimulus, loss resulted in abnormal activation...
Abstract Single-cell motility is spatially heterogeneous and driven by metabolic energy. Direct linking cell mobility to metabolism technically challenging but biologically important. Here we implemented a single-cell imaging assay measure glycolysis in individual endothelial cells using genetically-encoded biosensors capable of deciphering heterogeneity with subcellular resolution. We observed that cellular fuels activation, migration contraction the high lactate production sites...
Introduction: Recurrent malignant anastomotic strictures, especially close to the rectum, are challenging treat. Lumen-apposing metal stents (LAMS) have been increasingly used for benign strictures. Data in strictures is limited. We report a case successfully treated by non-cautery enhanced LAMS. Case Description/Methods: A 54-year-old man with history of rectosigmoid adenocarcinoma post low anterior resection, adjuvant chemoradiation, and local recurrence progression peritoneal...
Introduction: Biliary obstruction is common in patients with pancreatic cancer.Approximately 70% of present an obstruction.Endoscopic retrograde cholangiopancreatography(ERCP) the primary modality for biliary decompression.Alternative nonsurgical methods include endoscopic ultrasound guided drainage (EUSBD)via choledochoduodenostomy and percutaneous transhepatic drainage(PTBD).Our studies objective to review rates technical clinical success EUSBD PTBD malignant after unsuccessful ERCP...