A5051544202- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Wound Healing and Treatments
- Circular RNAs in diseases
- Mesenchymal stem cell research
- Diabetic Foot Ulcer Assessment and Management
- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Advanced MEMS and NEMS Technologies
- Advanced Fiber Optic Sensors
- Surgical site infection prevention
- Nerve injury and regeneration
- Nanoplatforms for cancer theranostics
- Toxin Mechanisms and Immunotoxins
- Epigenetics and DNA Methylation
- Head and Neck Surgical Oncology
- Galectins and Cancer Biology
- Coal Properties and Utilization
- Acupuncture Treatment Research Studies
- Ga2O3 and related materials
- Pain Mechanisms and Treatments
- Acoustic Wave Resonator Technologies
- Bat Biology and Ecology Studies
Zhongnan Hospital of Wuhan University
2022-2024
Wuhan University
2022-2024
Xi'an Technological University
2024
Changsha Hospital for Maternal and Child Health Care
2023
Union Hospital
2018-2022
Huazhong University of Science and Technology
2018-2022
Union Hospital
2019-2022
Wuhan Union Hospital
2021
Traffic Management Research Institute
2018
Beijing University of Chemical Technology
2016
Human adipose stem cells (ASCs) have emerged as a promising treatment paradigm for skin wounds. Recent works demonstrate that the therapeutic effect of is partially mediated by extracellular vesicles, which comprise exosomes and microvesicles. In this study, we investigate regenerative effects isolated microvesicles from ASCs evaluate mechanisms how ASC promote wound healing. Adipose cell-derived (ASC-MVs) were differential ultracentrifugation, stained PKH26, characterized electron...
Abstract Human adipose‐derived stem cells (ASCs) have a potential for the treatment of peripheral nerve injury. Recent studies demonstrated that can mediate therapeutic effect by secreting exosomes. We aimed to investigate human ASCs derived exosomes (ASC‐Exos) on regeneration in vitro and vivo. Our results showed after being internalized Schwann (SCs), ASC‐Exos significantly promoted SC proliferation, migration, myelination, secretion neurotrophic factors upregulating corresponding genes...
Oxidative damage is a critical cause of diabetic wounds. Exosomes from various stem cells could promote wound repair. Here, we investigated the potential mechanism by which exosomes adipose-derived (ADSC-EXOs) healing through modulation oxidative stress. We found that ADSC-EXOs proliferation, migration, and angiogenesis in keratinocytes, fibroblasts, endothelial cells. Furthermore, reduced reactive oxygen species (ROS) levels these protected them against hypoxic stress damage. Finally, local...
Colon cancer contributes to high mortality rates as the result of incomplete resection in tumor surgery. Multimodal imaging can provide preoperative evaluation and intraoperative image-guiding. As biocompatible nanocarriers, extracellular vesicles hold great promise for multimodal imaging. In this study, we aim synthesized an vesicles-based nanoprobe visualize colon with positron-emission tomography/computed tomography (PET/CT) near-infrared fluorescence (NIRF) imaging, investigated its...
Ischemic diseases caused by diabetes continue to pose a major health challenge and effective treatments are in high demand. Mesenchymal stem cells (MSCs) derived exosomes have aroused broad attention as cell-free treatment for ischemic diseases. However, the efficacy of from adipose-derived mesenchymal (ADSC-Exos) treating diabetic lower limb injury remains unclear.Exosomes were isolated ADSCs culture supernatants differential ultracentrifugation their effect on C2C12 HUVECs was assessed...
Non-healing trauma, with limited treatment options, remains a prevalent complication of diabetes mellitus. The underlying causes wherein include oxidative stress injury, bacterial infection, cellular dysfunction, and persistent inflammation. Acellular Dermal Matrix (ADM), wound dressing composed natural extracellular matrix abundant bioactive factors, has been successfully developed to treat various wounds, including burns diabetic ulcers. Protocatechualdehyde (PA) & trivalent iron ion...
Abstract Background Mesenchymal stem cells including adipose-derived (ASCs) have a considerable potential in the field of translational medicine. Unfortunately, multiple factors (e.g., older age, co-existing diabetes, and obesity) may impair cellular function, which hinders overall effectiveness autologous cell therapy. Noncoding RNAs—including microRNAs (miRNAs), long ncRNAs (lncRNAs), circular RNAs (circRNAs)—have been shown to play important roles biology. However, diabetes-related...
ABSTRACT Adipose-derived stem cells (ADSCs) have been widely applied in translational and regenerative medicine. During aging, there is a recognized functional decline ADSCs, which compromises their therapeutic effectiveness. Currently, the mechanisms of aging-induced cell dysfunction remain unclear, hence need to elucidate these propose strategies for reversing this impairment. In study, we found that ADSCs isolated from old donors (O-ADSCs) presented inferior phenotypes decreased...
Abstract Adipose stem cell (ASC)-based therapies provide an encouraging option for tissue repair and regeneration. However, the function of these cells declines with aging, which limits their clinical transformation. Recent studies have outlined involvement long non-coding RNAs in aging. Here, we reanalyzed our published RNA sequencing (RNA-seq) data profiling differences between ASCs from young old donors identified a lncRNA named double homeobox A pseudogene 10 (DUXAP10) as significantly...
Diabetes mellitus is a worldwide disease with high incidence. Diabetic peripheral neuropathy (DPN) one of the most common but often ignored complications diabetes that cause numbness and pain, even paralysis. Recent studies demonstrate Schwann cells (SCs) in nervous system play an essential role pathogenesis DPN. Furthermore, various transcriptome analyses constructed by RNA-seq or microarray have provided comprehensive understanding molecular mechanisms regulatory interaction networks...
Abstract Background Senescent adipose-derived stem cells (ASCs) exhibit reduced therapeutic efficacy during wound healing. Transcriptional regulation factors including long noncoding RNAs (lncRNAs) reportedly have essential roles in cell aging. However, the mechanisms of which lncRNAs influence mesenchymal aging and how it works need further investigation. Methods The expression patterns lncRNA senescence-associated RNA (SAN) miR-143-3p ASCs obtained from old young volunteer donors were...
Diabetic peripheral neuropathy (DPN) is considered to be the most frequent neuropathic complication of diabetes, and severely affects quality life patients. Long noncoding RNAs (lncRNAs) participate in various pathophysiological processes associate with many diseases. However, exact impact lncRNAs on DPN remains obscure. To discover a potential connection, microarray study was conducted analyze expression profiling messenger (mRNAs) dorsal root ganglia (DRG) from streptozotocin-induced...
GRB10 and its family members GRB7 GRB14 were important adaptor proteins. They regulated many cellular functions by interacting with various tyrosine kinase receptors other phosphorus-containing amino acid More more studies have shown that the abnormal expression of is closely related to occurrence development cancer. In our current research, data for 33 cancers from TCGA database was downloaded analysis. It found up-regulated in cholangiocarcinoma, colon adenocarcinoma, head neck squamous...
Abstract Background The functional impairment of adipose stem cells (ASCs) during aging limits their clinical transformation. Studies have shown that extrachromosomal circular DNAs (eccDNAs) are associated with tumor progression and cell aging, but the roles eccDNAs in ASCs remain unknown. Method We conducted Circle sequencing (Circle-seq) to identify isolated from young old donors. differentially expressed were calculated, annotated validated via polymerase chain reaction. Results Thousands...
Circular RNAs (circRNAs) play a vital role in diabetic peripheral neuropathy. However, their expression and function Schwann cells individuals with neuropathy remain poorly understood. Here, we performed protein profiling circRNA sequencing of sural nerves patients controls. Protein revealed 265 differentially expressed proteins the group. Gene Ontology indicated that were mainly enriched myelination mitochondrial oxidative phosphorylation. A real-time polymerase chain reaction assay to...
Adipose-derived stem cells (ADSCs) can differentiate into Schwann (SCs) at the site of nerve injury, where cell-derived exosomes (SC-Exos) are suspected to exert an induction effect. Our study aimed induce differentiation ADSCs in vitro using SC-Exos and investigate mechanisms involved through miRNA sequencing. Subcutaneous fat was used extract ADSCs. Exosomes were extracted from cell lines (RSC96) ultracentrifugation able be taken up by human After 8 days SC-Exos, phenotypic characteristics...
Diabetic peripheral neuropathy is a common complication associated with diabetes mellitus pathogenesis that incompletely understood. By regulating RNA silencing and post-transcriptional gene expression, microRNAs participate in various biological processes human diseases. However, the relationship between progress of diabetic still lacks thorough exploration. Here we used microarray microRNA mRNA expression profiling to analyze mRNAs which are aberrantly expressed dorsal root ganglia from...
Background: Despite ample evidence demonstrating that anterior cruciate ligament (ACL) and meniscus tears are associated with posttraumatic osteoarthritis (PTOA) development, the contributing factors remain unknown. Synovial inflammation has recently been recognized as a pivotal factor in pathogenesis of OA. However, there is lack data on synovial profiles after ACL or injuries, which may contribute to PTOA. Methods: Twelve patients and/or injuries were recruited. During surgery, tissues...
The parasitoid fly Ormia ochracea shows an astonishing localization ability with its tiny hearing organ. A novel MEMS biomimetic acoustic pressure gradient sensitive structure was designed and fabricated by mimicking the mechanically coupled tympana of fly. Firstly, analytic representation formulas resultant force moment incoming plane wave acting on were derived. After that, modal analysis performed results show that has out-of-phase in-phase vibration modes, corresponding eigenfrequency is...
Based on the silicon-on-insulator (SOI) silicon, a novel resonant pressure sensor for atmosphere detection is designed and fabricated. The resonator suspended by four beams at pedestals diaphragm encapsulated between two glass lids. shape changes with change of ambient gas pressure; thereby resonator’s nature frequency also changes. To prevent undesired etching during wet etching, protective process using silicon nitride oxide adopted. Experiments show that after 10 hours in tetramethyl...