A5018331168- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Enterobacteriaceae and Cronobacter Research
- Transgenic Plants and Applications
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Child Nutrition and Water Access
- Infant Nutrition and Health
- Digestive system and related health
- Salmonella and Campylobacter epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Public Health and Environmental Issues
- Business, Innovation, and Economy
- Health Systems, Economic Evaluations, Quality of Life
- Ethics and bioethics in healthcare
- Lysosomal Storage Disorders Research
- Vibrio bacteria research studies
Universidad Científica del Sur
2019-2023
Universidad Peruana Cayetano Heredia
2010-2023
University of Buenos Aires
2012-2015
Instituto de Medicina Tropical
2011
ABSTRACT Enterotoxigenic Escherichia coli (ETEC) is a major cause of childhood diarrhea. The present study sought to determine the prevalence and distribution toxin types, colonization factors (CFs), antimicrobial susceptibility ETEC strains isolated from Peruvian children. We analyzed children between 2 24 months age in passive surveillance study. Five E. colonies per patient were studied by multiplex real-time PCR identify virulence factors. ETEC-associated toxins confirmed using GM1-based...
Introducción. Las E. coli diarrogénicas (DEC) son una de las principales causas diarrea en niños países vías desarrollo. Sin embargo, no rutinariamente diagnosticadas los laboratorios clínicos. Objetivos. Determinar la prevalencia DEC peruanos y describir variabilidad genética estas cepas. Materiales métodos. Se utilizaron 8 003 cepas previamente aisladas ocho estudios previos niños, mayormente zonas periurbanas Lima. El diagnóstico fue a través un PCR múltiple tiempo real para seis grupos...
ABSTRACT This study aims to characterize the presence of virulence factors enterotoxigenic Escherichia coli (ETEC) causing traveler's diarrhea. Among 52 ETEC isolates, most common toxin type was STh, and frequent colonization (CFs) were CS21, CS6, CS3. On other hand, nonclassical EAST1 EatA frequently present.
Antibiotic resistance is increasing worldwide, being of special concern in low- and middle-income countries. The aim this study was to determine the antimicrobial susceptibility mechanisms 205 enterotoxigenic Escherichia coli (ETEC) isolates from two cohort studies children <24 months Lima, Peru. ETEC were identified by an in-house multiplex real-time PCR. Susceptibility 13 agents tested disk diffusion; evaluated resistant ampicillin (64%), cotrimoxazole (52%), tetracycline (37%); 39%...
We conducted a prospective study in three hospitals Lima human immunodeficiency virus (HIV) children to determine the frequency of diarrheagenic Escherichia coli. Five E. coli colonies/patients were studied by multiplex real-time polymerase chain reaction identify six currently recognized groups diarrhea-associated . have analyzed 70 HIV-associated diarrheal and control samples from HIV-infected without diarrhea. Among episodes 19% persistent, 3% dysenteric, 33% associated with moderate or...
The aim of this study was to determine the prevalence, virulence factors (stx, eae, ehxA and astA) phylogenetic relationships [PFGE multilocus sequence typing (MLST)] Shiga toxin-producing Escherichia coli (STEC) strains isolated from four previous cohort studies in 2212 Peruvian children aged <36 months. STEC prevalence 0.4 % (14/3219) diarrhoeal 0.6 (15/2695) control samples. None infected developed haemolytic uraemic syndrome (HUS) or other complications STEC. stx1 present 83 strains,...
In an active diarrhea surveillance study of children aged 12-24 months in Lima, Peru, norovirus was the most common pathogen identified. The percentage mixed (bacterial and noroviral) infections significantly higher among norovirus-positive samples (53%) than norovirus-negative (12%). combination with bacterial pathogens associated increased clinical severity over that either single-pathogen or infections.
Norovirus was detected in 17.4% of 224 diarrhoeal samples from children younger than 24 months age Lima, whom all common pathogens had been excluded (pathogen negative). identified more frequently older 12 (34% vs 8%, P<0.001). Among norovirus-positive samples, genogroup II the predominant group (92%). Compared with rotavirus, norovirus episodes tended to be shorter duration and less severe. The role as a cause diarrhoea ascertainment its severity developing countries needs further...
Secretory diarrhea caused by cholera toxin (CT) is initiated binding of CT’s B subunit (CTB) to GM1-ganglioside on the surface intestinal cells. Lactoferrin, a breast milk glycoprotein, has shown protective effect against several enteropathogens. The aims this study were determine bovine-lactoferrin (bLF) CT-induced fluid accumulation in mice, and interaction between bLF CT/CTB with receptor. Fluid induced CT was evaluated mouse ileal loop model using 56 BALB/c without added before, after or...
We analyzed a randomly selected group of 30 diffusely adherent (DAEC), enteropathogenic, enteroaggregative, and five Shiga toxin-producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. (ETEC) colonization factors (CFs) were evaluated by dot-blot assay using 21 CF-specific monoclonal antibodies. Out 95 non-ETEC strains, three DAEC found to express surface antigen 20 (CS20). No other expressed CFs. confirmed the CS20-positive as ETEC-negative repeat PCR...
Introducción. Las E. coli de adherencia difusa (DAEC) son el sexto grupo diarrogénicas reconocidas. Su asociación con diarrea es controversial. No se conoce la variabilidad en los patrones cepas clínicas. Objetivos. Comparar entre aisladas niños y sin diarrea. Materiales métodos. Se analizó 31 DAEC, 25 6 asintomáticos (control) aislados un estudio cohorte menores 12 meses cono sur Lima. DAEC fueron identificadas por PCR (gen daaD). evaluó patrón grado cultivos células HEp-2; polimerización...
Enterotoxigenic Escherichia coli (ETEC) ranks among the most relevant diarrheagenic pathogens. Efforts to design vaccines fight ETEC have been focused on colonizing factors (CFs) and atypical virulence (AVF). An effective vaccine must account for differences in regional prevalence of these CFs AVFs be truly a given area. In present study, presence 16 9 AVFs, as well heat-stable (ST) variants (STh or STp), was established by polymerase chain reaction 205 Peruvian isolates (120 from diarrhea...
The objective of the study was to describe characteristics Clinical Trials (CT) supervised by Institute Health Technology Assessment and Research carried out in EsSalud between 2015 2018 main observations supervisions completed. A descriptive 82 clinical trials conducted 2018. Most were phase III studies (81.7%); most frequent route administration products oral (47.6%), sponsored pharmaceutical industry (96.3%). those related contract (83.8%), overhead payment (57.3%), lack regulatory...
Background.Quinolones and azithromycin remain as drugs of choice for the treatment Enterotoxigenic Escherichia coli (ETEC) infections, especially traveleŕs diarrhea in Peru.The aim this study was to determine mechanisms quinolone resistance ETEC strains isolated from Peruvian children.Methods.We analyzed a total 205 previously two cohort studies children <24 months age Lima, Peru.ETEC identified by multiplex real-time PCR lt and/or stgenes.Susceptibility nalidixic acid (Nal)
Introduction. Diarrheagenic E. coli (DEC) are a major cause of diarrhea in children developing countries. However, they not part routine diagnosis clinical laboratories. Objectives. To determine the DEC prevalence Peruvian and to describe genetic variability these strains. Materials methods. A total 8 003 strains previously isolated from eight different studies children, mainly peri-urban areas Lima, were analyzed. Diagnosis was done with Multiplex real-time PCR using genes for each 6...
Introduction. Diffusely adherent E. coli (DAEC) is the sixth recognized group of diarrheagenic coli. However, its association with diarrhea remains controversial. Variability in adherence patterns clinical strains unknown. Objectives. To compare between isolated from children and without diarrhea. Materials methods. A total 31 DAEC were analyzed, 25 6 asymptomatic (control) children, a cohort study under one year age southern districts Lima. identified by PCR (daaD gene). The pattern score...
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