A5024911505- Pregnancy and preeclampsia studies
- Epigenetics and DNA Methylation
- Parathyroid Disorders and Treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- Birth, Development, and Health
- Genetic Syndromes and Imprinting
- Renal and related cancers
- Pluripotent Stem Cells Research
- Ultrasound and Hyperthermia Applications
- Aortic Disease and Treatment Approaches
- Cardiomyopathy and Myosin Studies
- Animal Genetics and Reproduction
- Congenital heart defects research
- Fuel Cells and Related Materials
- Cardiac Valve Diseases and Treatments
- Reproductive System and Pregnancy
- Cancer-related molecular mechanisms research
- Magnesium in Health and Disease
- CRISPR and Genetic Engineering
- Medical Imaging and Pathology Studies
- Genetics, Aging, and Longevity in Model Organisms
- Sperm and Testicular Function
Tufts Medical Center
2018-2024
Tufts University
2020-2024
University of Massachusetts Amherst
2013-2023
Infant
2023
University of Washington
2012-2021
HR Wallingford
2019
Chulalongkorn University
2016
University of Colorado Denver
2013
Elevated serum phosphate has emerged as a major risk factor for vascular calcification. The sodium-dependent cotransporter, PiT-1, was previously shown to be required phosphate-induced osteogenic differentiation and calcification of cultured human smooth muscle cells (VSMCs), but its importance in vivo the potential role homologue, PiT-2, have not been determined. We investigated requirement PiT-1 using mouse model chronic kidney disease compensatory PiT-2 vitro knockdown overexpression...
Vascular smooth muscle cells (SMCs) are major precursors contributing to osteochondrogenesis and calcification in atherosclerosis. Runt-related transcription factor-2 (Runx2) has been found essential for SMC differentiation an osteochondrogenic phenotype subsequent vitro. A recent study using a conditional targeting allele that produced truncated Runx2 protein SMCs of ApoE−/− mice showed reduced vascular calcification, likely occurring via reduction receptor activator nuclear factor-κB...
Idiopathic basal ganglia calcification is a brain disorder that has been genetically linked to autosomal dominant mutations in the sodium-dependent phosphate co-transporter, SLC20A2. The mechanisms whereby deficiency of Slc20a2 leads ganglion are unknown. In mouse brain, we found was expressed tissues produce and/or regulate cerebrospinal fluid, including choroid plexus, ependyma and arteriolar smooth muscle cells. Haploinsufficient +/- mice developed age-dependent formed glymphatic...
Vascular calcification is the deposition of calcium phosphate minerals in vascular tissue. occurs by both active and passive processes. Extent tissue-specific patterns are predictors cardiovascular morbidity mortality. The placenta a highly vascularized organ with specialized vasculature that mediates communication between two circulatory systems. At delivery often contains calcified tissue can be considered marker viral infection, but mechanisms, histoanatomical specificity,...
Although successful implantation is required for development in placental mammals, the molecular and morphogenetic events that define peri-implantation remain largely unexplored.Here we present detailed morphological immunohistochemical analysis of mouse embryos between embryonic day 3.75 5.25 gestation, during process vivo. We examined expression patterns key transcription factors (Sox2, Oct4, Nanog, Cdx2, Gata6, Sox17, Yy1) pre- postimplantation development. Additionally, changes through...
Mouse embryos lacking the polycomb group gene member Yin-Yang1 (YY1) die during peri-implantation stage. To assess post-gastrulation role of YY1, a conditional knock-out (cKO) strategy was used to delete YY1 from visceral endoderm yolk sac and definitive embryo. cKO display profound defects at 9.5 days post coitum (dpc), including disrupted angiogenesis in mesoderm derivatives altered epithelial characteristics endoderm. Significant changes both cell death proliferation were confined...
The X-linked gene Rlim plays major roles in female mouse development and reproduction, where it is crucial for the maintenance of imprinted X chromosome inactivation extraembryonic tissues embryos. However, while females carrying a systemic knockout (KO) die around implantation, male KO mice appear healthy are fertile. Here, we report an important role testis highly expressed post-meiotic round spermatids as well Sertoli cells. Systemic deletion results lower numbers mature sperm that...
The placenta mediates the transport of nutrients, such as inorganic phosphate (Pi), between maternal and fetal circulatory systems. itself also requires high levels nutrient uptake it develops to provide critical support for development. This study aimed determine placental Pi mechanisms using in vitro vivo models. We observed that (P33) BeWo cells is sodium dependent SLC20A1/Slc20a1 most highly expressed sodium-dependent transporter mouse (microarray), human cell line (RT-PCR) term...
In female mice, the gene dosage from X chromosomes is adjusted by a process called chromosome inactivation (XCI) that occurs in two steps. An imprinted form of XCI (iXCI) silences paternally inherited (Xp) initiated at 2- to 4-cell stages. As extraembryonic cells including trophoblasts keep Xp silenced, epiblast give rise embryo proper reactivate and undergo random (rXCI) around implantation. Both iXCI rXCI require lncRNA Xist , which expressed be inactivated. The X-linked E3 ubiquitin...
Yinyang1 (YY1) participates in protein-DNA, protein-RNA, and protein-protein interactions regulates developmental processes disease mechanisms. YY1 regulate a range of important biological functions, including oocyte maturation, epithelial to mesenchymal transition, vascular endothelial growth factor (VEGF) signaling. We tested the hypothesis that is required for inner cell mass (ICM) lineage commitment during preimplantation development. In this study, we document gene expression patterns...
The placenta is a highly vascularised organ with unique structural and metabolic complexities. As the primary conduit of fetal support, mediates transport oxygen, nutrients, waste between maternal blood. Thus, normal anatomy physiology absolutely required for maintenance health during pregnancy. Moreover, impaired placental can negatively impact offspring growth trajectories as well increase risk cardiovascular disease later in life. Despite these crucial roles placenta, disorders, such...
Inorganic phosphate (Pi) is an essential nutrient that fulfills critical roles in human health. It enables skeletal ossification, supports cellular structure and organelle function, serves key biochemical energetics molecular signaling. Pi homeostasis modulated through diet, intestinal uptake, renal reabsorption, mobilization of stores bone extracellular compartments. Disrupted associated with wasting, mineral disorders, vascular calcification. Mechanisms pregnancy remain incompletely...
The sodium-dependent phosphate transporter, SLC20A1, is required for elevated inorganic (Pi) induced vascular smooth muscle cell (VSMC) matrix mineralization and phenotype transdifferentiation. Recently, Pi was shown to induce ERK1/2 phosphorylation through SLC20A1 by uptake-independent functions in VSMCs, suggesting a signaling response Pi. Previous studies identified Rap1 guanine nucleotide exchange factor (RapGEF1) as an SLC20A1-interacting protein RapGEF1 promotes activation. In this...