A5015590745- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Birth, Development, and Health
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Prenatal Screening and Diagnostics
- dental development and anomalies
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Gender, Education, and Development Issues
- Agricultural pest management studies
- Agricultural Development and Management
- Genetic Syndromes and Imprinting
- Dietary Effects on Health
- CRISPR and Genetic Engineering
- Reproductive Health and Technologies
- Demographic Trends and Gender Preferences
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Effects and risks of endocrine disrupting chemicals
- Sexual Differentiation and Disorders
- Agricultural Innovations and Practices
- Morphological variations and asymmetry
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
University of Massachusetts Amherst
2014-2023
Long noncoding RNAs (lncRNAs) exhibit diverse functions, including regulation of development. Here, we combine genome-wide mapping SMAD3 occupancy with expression analysis to identify lncRNAs induced by activin signaling during endoderm differentiation human embryonic stem cells (hESCs). We find that DIGIT is divergent Goosecoid (GSC) and expressed differentiation. Deletion the SMAD3-occupied enhancer proximal inhibits GSC definitive Disruption gene encoding depletion transcript reveal...
Preconception environmental conditions have been demonstrated to shape sperm epigenetics and subsequently offspring health development. Our previous findings in humans showed that urinary anti-androgenic phthalate metabolites males were associated with altered methylation blastocyst-stage embryo To corroborate this, we examined the effect of preconception exposure di(2-ethylhexyl) (DEHP) on genome-wide DNA gene expression profiles mice. Eight-week old C57BL/6J male mice exposed either a...
Abstract Parental age at time of offspring conception is increasing in developed countries. Advanced male associated with decreased reproductive success and increased risk adverse neurodevelopmental outcomes offspring. Mechanisms for these effects remain unclear, but changes sperm DNA methylation over one potential explanation. We assessed genome-wide from 47 semen samples collected participants couples seeking infertility treatment. report that higher was lower likelihood fertilization live...
Abstract With readily available transcriptome-wide data, understanding the role of each expressed gene is an essential next step. Although RNAi technologies allow for genome-wide screens in cell culture, these approaches cannot replace strategies discovery embryo. Here we present, first time, a knockdown screen mouse preimplantation embryos. Early mammalian development encompasses dynamic cellular, molecular and epigenetic events that are largely conserved from to man. We assayed 712 genes...
Aims: Paternal age is increasing in developed countries. Understanding of aging-related epigenetic changes sperm needed as well factors that modify such changes. Materials & methods: Young pubertal and mature rats were exposed perinatally to vehicle or environmental xenobiotic 2,2′,4,4′-tetrabromodiphenyl ether. Epididymal was reduced representation bisulfite sequenced. Differentially methylated regions (DMRs) identified via MethPipe. Results: In control animals, 5319 age-dependent DMRs...
Appropriate epigenetic regulation of gene expression during lineage allocation and tissue differentiation is required for normal development. One example genomic imprinting, which defined as parent-of-origin mono-allelic expression. Imprinting established largely due to differences arriving in the zygote from sperm egg haploid genomes. In mouse, there are approximately 150 known imprinted genes, many occur clusters that regulated together. cluster includes maternally expressed Igf2r,...
SUMMARY Nucleolar protein 2 (NOP2) is evolutionarily conserved from yeast to human, and has been found play an important role in accelerating cell proliferation, cell‐cycle progression, tumor aggressiveness. The expression pattern function of Nop2 during early mammalian embryo development, however, not investigated. We identified as essential gene for development the blastocyst stage while performing RNA interference (RNAi)‐based screen mouse preimplantation embryos. expressed throughout...
Mediator is an evolutionarily conserved multi-subunit complex, bridging transcriptional activators and repressors to the general RNA polymerase II (Pol II) initiation machinery. Though complex crucial for transcription of almost all Pol promoters in eukaryotic organisms, phenotypes individual subunit mutants are each distinct. Here, we report first time, essential role MED20 early mammalian embryo development. Although Med20 mutant mouse embryos exhibit normal morphology at E3.5 blastocyst...
Abstract Adaptive phenotypes are shaped by a combination of genetic and environmental forces, but how they interact remains poorly understood. Here, we utilize the cichlid oral jaw apparatus to better understand these gene‐by‐environment effects. First, employed RNA‐seq in bony ligamentous tissues important for opening identify differentially expressed genes between species across foraging environments. We used two Lake Malawi adapted different habitats along pelagic–benthic ecomorphological...
Aim: Accumulating evidence associates sperm mitochondria DNA copy number (mtDNAcn) with male infertility and reproductive success. However, the mechanism underlying mtDNAcn variation is largely unknown. Patients & methods: Sperm genome-wide methylation were assessed using triplex probe-based quantitative PCR Illumina's 450K array, respectively. Multivariable models association between profiles of 47 men seeking treatment. Results: A priori candidate-gene approach showed was associated 16...
Advanced paternal age at fertilization has been suggested to be a risk factor for neurodevelopmental, psychiatric and other disorders in offspring. One emerging hypothesis suggests that altered offspring phenotype is linked with age-related accumulation of epigenetic changes the sperm fathers. Given increasing developed world, understanding aging-related needed as well environmental factors modify such changes. In this study, we characterize age-dependent DNA methylation profiles between...
Adaptive phenotypes are shaped by a combination of genetic and environmental forces, while the literature is rich with studies focusing on either genetics or environment contributions, those that consider both rare. Here we utilize cichlid oral jaw apparatus to fill this knowledge-gap. First, employed RNA-seq in bony ligamentous tissues important for opening identify differentially expressed genes between species across foraging environments. Our treatments were designed force animals employ...