A5014896642- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Glycosylation and Glycoproteins Research
- Virus-based gene therapy research
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Protein purification and stability
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Veterinary Oncology Research
- Animal Virus Infections Studies
- Antimicrobial Resistance in Staphylococcus
- Lymphoma Diagnosis and Treatment
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Bacterial biofilms and quorum sensing
- Toxin Mechanisms and Immunotoxins
- Nanoparticle-Based Drug Delivery
- Acoustic Wave Resonator Technologies
- Molecular Junctions and Nanostructures
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Mechanical and Optical Resonators
- Viral-associated cancers and disorders
University of Lisbon
2015-2025
Centre for Research in Anthropology
2020-2024
Universidade Lusófona do Porto
2024
Instituto Nacional de Investigação Agrária e Veterinária
2022
TechnoPhage (Portugal)
2012-2017
Instituto de Medicina Molecular João Lobo Antunes
2012
Scripps Research Institute
2005
UPMC Hillman Cancer Center
2005
Cancer Research Center
2005
Instituto Gulbenkian de Ciência
2005
Staphylococcus aureus biofilm-associated infections are a major public health concern. Current therapies hampered by reduced penetration of antibiotics through biofilm and low accumulation levels at infected sites, requiring prolonged usage. To overcome these, repurposing in combination with nanotechnological platforms is one the most appealing fast-track cost-effective approaches. In present work, we assessed potential therapeutic benefit three antibiotics, vancomycin, levofloxacin...
Extracellular hemoglobin, a byproduct of hemolysis, can release its prosthetic heme groups upon oxidation. This produces metabolically active that is exchangeable between acceptor proteins, macromolecules and low molecular weight ligands, termed here labile heme. As it accumulates in plasma acts pro-oxidant manner regulates cellular metabolism while exerting pro-inflammatory cytotoxic effects foster the pathogenesis hemolytic diseases. Here, we developed characterized panel heme-specific...
The human apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G), also known as CEM-15, is a host-cell factor involved in innate resistance to retroviral infection. HIV-1 viral infectivity (Vif) protein was shown protect the virus from APOBEC3G-mediated cDNA hypermutation. mechanism proposed for protection of by Vif mediated APOBEC3G degradation through ubiquitination and proteasomal pathway. Here we show that Escherichia coli APOBEC3G-induced cytidine deamination...
The delivery of therapeutic molecules to the central nervous system is hampered by poor across blood-brain barrier (BBB). Several strategies have been proposed enhance transport into brain, including invasive techniques and receptor-mediated (RMT). Both approaches several drawbacks, such as BBB disruption, receptor saturation, off-target effects, raising safety issues. Herein, we show that specific domains Dengue virus type 2 capsid protein (DEN2C) can be used trans-BBB peptide vectors....
Abstract Background Sepsis is a severe condition associated with high prevalence and mortality rates. Parvovirus enteritis predisposing factor for sepsis, as it promotes intestinal bacterial translocation immunosuppression. This makes dogs infected by parvovirus suitable study population far sepsis concerned. The main objective of the present was to evaluate differences between two sets SIRS (Systemic Inflammatory Response Syndrome) criteria in outcome prediction: 1991 2001. possibility...
Abstract Antibody–drug conjugates (ADCs) are a new class of therapeutics that combine the lethality potent cytotoxic drugs with targeting ability antibodies to selectively deliver cancer cells. In this study we show for first time synthesis reactive‐oxygen‐species (ROS)‐responsive ADC (VL‐DAB31‐SN‐38) is highly selective and B‐cell lymphoma (CLBL‐1 cell line, IC 50 value 54.1 nM). The was possible due discovery diazaborines (DABs) very effective ROS‐responsive unit also stable in buffer...
Canine lymphoma is one of the most common and aggressive hematopoietic tumors in dogs. Despite recent advances veterinary cancer treatments, lack specificity, side effects, resistance to conventional chemotherapies has opened an urgent need develop more targeted safe therapeutics address this unmet Thus, present study, we aimed generate a new class based on recombinant single-domain antibody (sdAb) immunotoxin derived from PE38 Pseudomonas aeruginosa exotoxin A. For purpose, fused toxin with...
Non-Hodgkin lymphoma (NHL) is one of the most common causes cancer-related death in United States and Europe. Although outcome NHL patients has improved over last years with current therapies, rate mortality still high. A plethora new drugs entering clinical development for treatment; however, approval treatments remains low due part to paucity clinically relevant models validation. Canine shares remarkable similarities its human counterpart, making dog an excellent animal model explore...
A major bottleneck in the successful development of central nervous system (CNS) drugs is discovery and design molecules that can cross blood-brain barrier (BBB). Nano-delivery strategies are a promising approach take advantage natural portals entry into brain such as monoclonal antibodies (mAbs) targeting endogenous BBB receptors. However, main selected mAbs rely on broadly expressed receptors, transferrin insulin selection processes do not fully mimic native receptor conformation, leading...
To test the cross-immunogenicity of anti-CT-P13 IBD patients' sera to CT-P13/infliximab originator and comparative antigenicity evoked by sera.Sera patients with measurable antibodies were tested for their cross-reactivity 5 batches infliximab CT-P13. Anti-drug antibody positive from treated used compare antigenic epitopes.All 42 37 anti-infliximab cross-reactive CT-P13 respectively. Concentration anti-drug against or strongly correlated both IgG1 IgG4 (P < 0.001). Anti-CT-P13 (n = 32)...
Antibody-drug conjugates (ADCs) are among the fastest-growing classes of therapeutics in oncology. Although ADCs spotlight, they still present significant engineering challenges. Therefore, there is an urgent need to develop more stable and effective ADCs. Most rabbit light chains have extra disulfide bridge, that links variable constant domains, between Cys80 Cys171, which not found human or mouse. Thus, a new generation ADCs, we explored potential rabbit-derived VL-single-domain antibody...
Lentiviral vectors are among the most efficient tools for gene delivery into mammalian cells. A major goal of lentiviral systems is to develop that can efficiently target specific cell types. In present work, we attempt generate viral particles targeting delivery. We have used CCR5-positive cells as our strategy. Therefore, developed a novel Sindbis pseudotyped vector where receptor binding envelope protein was modified directly encode single-chain antibody fragment (scFv) against CCR5...