A5032591855- Reproductive tract infections research
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Microbial infections and disease research
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Blood groups and transfusion
- Legionella and Acanthamoeba research
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- T-cell and B-cell Immunology
- COVID-19 Clinical Research Studies
- Bacterial Infections and Vaccines
- COVID-19 epidemiological studies
- Genital Health and Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Axon Guidance and Neuronal Signaling
- Urinary Tract Infections Management
- Neonatal Health and Biochemistry
- Urological Disorders and Treatments
- Hepatitis B Virus Studies
Cardiff University
2015-2025
University Hospital of Wales
2015-2025
Cwm Taf University Health Board
2019-2024
Princess of Wales Hospital
2024
Royal Glamorgan Hospital
2015-2022
Blackwood
2021
Virgin Care
2016
University of Oxford
2002
Oxford Centre for Diabetes, Endocrinology and Metabolism
2001
University of Maryland, Baltimore
2001
We studied the prevalent cytotoxic CD8 T cell response mounted against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike glycoprotein
The transcription factor PAX6 has been implicated in forebrain patterning,cerebral cortical arealization and development of thalamocortical connections. Using a Pax6/lacZ knockout mouse, which the endogenous Pax6 expression is reflected by β-galactosidase activity, we have studied consequences loss Pax6function on (TCA) corticofugal axon (CFA) pathfinding during period embryonic day (E) 14.5 to E18.5. Carbocyanine dye tracing heterozygotes (Pax6+/-) Pax6wild-type (Pax6+/+) brains revealed...
Type 2 diabetes is characterized by diminished or inappropriate secretion of insulin, which could be a defect either islet cell function beta-cell mass. Quantitation populations in postmortem pancreas demonstrates little change mass type diabetes. Reduction (up to 30%) associated largely with amyloid deposition, and the degree amyloidosis independent duration disease. Insulin secretory capacity dependent on both cells. beta-Cell heterogeneous; increasing glucose concentrations result...
ABSTRACT Ureaplasma spp. are associated with numerous clinical sequelae treatment options being limited due to patient and pathogen factors. This report examines the prevalence mechanisms of antibiotic resistance among strains isolated from 95 neonates, 32 women attending a sexual health clinic, 3 patients under investigation for immunological disorders, between 2007 2013 in England Wales. MICs were determined by using broth microdilution assays, subset isolates compared method Mycoplasma...
CD8+ T cells destroy insulin-producing pancreatic β in type 1 diabetes through HLA class I-restricted presentation of self-antigens. Combinatorial peptide library screening was used to produce a preferred recognition landscape for patient-derived cell receptor (TCR) that recognized the preproinsulin-derived (PPI-derived) sequence LWMRLLPLL context disease risk allele A*24:02. Data were generate strong superagonist peptide, enabling production an autoimmune A*24:02-peptide-TCR structure by...
<title>Abstract</title> Older adults are particularly vulnerable to infectious diseases, and vaccines often less effective in this population due immunosenescence, which is characterized by diminished B T memory responses. Autophagy believed underlie many facets of cellular aging, including immunosenescence. It crucial for maintaining cell functions but declines with age, along the endogenous metabolite spermidine that helps maintain autophagy levels. We conducted a double-blind, randomized,...
Abstract The genital mycoplasmas are a unique group of inherently antibiotic-resistant sexually transmitted bacteria, often associated with non-gonococcal urethritis and bacterial vaginosis. MYCO WELL D-ONE is culture-based assay that aims to detect these organisms whilst concurrently screening them for antibiotic resistance. Urine and/or swabs from 856 informed consented participants attending Welsh sexual health clinics were subjected analysis, alongside qPCR culture titration...
A minimal genome and absent bacterial cell wall render Mycoplasma hominis inherently resistant to most antimicrobials except lincosamides, tetracyclines, fluoroquinolones. Often dismissed as a commensal (except where linked preterm birth), it causes septic arthritis in immunodeficient patients is increasingly associated with transplant failure (particularly lung) accompanying immunosuppression. We examined antimicrobial susceptibility (AST) on strains archived from 2005 2015 submitted the...
ABSTRACT The adaptive immune system protects against infection via selection of specific antigen receptors on B-cells and T-cells. We studied the prevalent CD8 ‘killer’ T-cell response mounted SARS-CoV-2 Spike 269-277 epitope YLQPRTFLL most frequent Human Leukocyte Antigen (HLA) class I worldwide, HLA A*02. widespread P272L mutation has arisen in at least 14 different lineages to date, including identified as variants concern. was common B.1.177 lineage associated with establishing ‘second...
To evaluate the accuracy, susceptibility and specificity of MYCOPLASMA IST3, next generation most popular culture-based in vitro diagnostic device designed to detect, identify test urogenital mycoplasma infections.MYCOPLASMA IST3 was evaluated against culture- molecular-based gold standard methodologies identify, enumerate determine antimicrobial resistance for Mycoplasma hominis Ureaplasma species 516 clinical samples collected across France, Serbia UK. Sample types included...
mRNA vaccine technologies introduced following the SARS-CoV-2 pandemic have highlighted need to better understand interaction of adjuvants and early innate immune response. Type I interferon (IFN-I) is an integral part this response that primes several components adaptive Women are widely reported respond than men tri- quadrivalent influenza vaccines. Plasmacytoid dendritic cells (pDCs) primary cell type responsible for IFN-I production, female pDCs produce more male since upstream pattern...
Central to sex differences observed in outcome from infection and vaccination is the innate immune response, specifically production of type I interferons by plasmacytoid dendtiric cells (pDCs), main producers IFN-α. Evaluation IFN-α pDCs therefore critical for studies function. However, reliable measurement pDC hampered reduced cell yields cytokine after cryopreservation or even short delays stimulating freshly isolated cells. We here describe a simple yet robust method measuring that...
Introduction Family studies of antiviral immunity provide an opportunity to assess virus-specific in infected and highly exposed individuals, as well examine the dynamics viral infection within families. Transmission SARS-CoV-2 between family members represented a major route for spread during early stages pandemic, due nature transmission through close contacts. Methods Here, humoral cellular is explored 264 infected, or unexposed individuals from 81 families United Kingdom sampled winter...
Often dismissed as a commensal, Mycoplasma hominis is an increasingly prominent target of research due to its role in septic arthritis and organ transplant failure immunosuppressed patients, particularly lung transplantation. As mollicute, highly reductive genome structure render it refractile most forms treatment growing levels resistance the few sources left, such fluoroquinolones. We examined antimicrobial susceptibility (AST) fluoroquinolones on 72 isolates observed three (4.1%), with...
Abstract mRNA vaccine technologies introduced following the SARS-CoV-2 pandemic have highlighted need to better understand interaction of adjuvants and early innate immune response. Interferon type I (IFN-I) is an integral part this response can prime several components adaptive Females are widely reported respond than males seasonal tri- quad-valent influenza vaccines. Plasmacytoid dendritic cells (pDCs) primary cell responsible for IFN-I production female pDCs produce more male since...