A5084239387- Genomics and Chromatin Dynamics
- T-cell and B-cell Immunology
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Collagen: Extraction and Characterization
- Cellular Mechanics and Interactions
- Connective tissue disorders research
- Cell Adhesion Molecules Research
- Vector-Borne Animal Diseases
- Diabetes and associated disorders
- Enzyme Production and Characterization
- Advanced biosensing and bioanalysis techniques
- Digital Innovation in Industries
- Economic Theory and Institutions
- Animal Disease Management and Epidemiology
- Neurogenesis and neuroplasticity mechanisms
- Airway Management and Intubation Techniques
- Nerve injury and regeneration
- Caveolin-1 and cellular processes
- Computational Drug Discovery Methods
- Agriculture and Farm Safety
- Ionic liquids properties and applications
- Proteoglycans and glycosaminoglycans research
Friedrich-Loeffler-Institut
2024
Simon Fraser University
2019-2020
Utrecht University
2020
Heidelberg University
2017-2019
German Cancer Research Center
2017-2019
Heinrich Heine University Düsseldorf
2019
DKFZ-ZMBH Alliance
2017
Evonik (Germany)
2016
Technical University of Munich
2015
Humboldt-Universität zu Berlin
2012-2014
Over the last decade ionic liquids have achieved much attention and are not any longer just a class of esoteric compounds, but proving to be valuable useful in multitude different applications. So far, mainly been considered an alternative conventional solvents reaction separation processes. Hence, it is surprising that most recent publications report on use as solvent for chemical biochemical syntheses. This may rationalized by possibility carrying out biphasic reactions recovery otherwise...
One of the most controversial questions in immunology is molecular basis by which Th lymphocytes deliver activating signals to quiescent B during T cell-dependent immune responses. Recent studies suggest that activation may involve signaling mediated direct helper cell-B cell contact. Since membrane-associated MHC-encoded class II molecules (Ia) must be recognized for generation humoral responses, they are obvious candidates receptors this signal. Here we report stimulation murine cells with...
Nucleosomes play a dual role in compacting the genome and regulating access to DNA. To unravel underlying mechanism, we study fluorescently labeled mononucleosomes by multi-parameter FRET measurements characterize their structural dynamic heterogeneity upon NaCl-induced destabilization. Species-selective fluorescence lifetime analysis photon distribution reveal intermediates during nucleosome opening lead coherent kinetic model. In octasomes hexasomes interface between H2A-H2B dimers...
Chromatin compaction and gene accessibility are orchestrated by assembly disassembly of nucleosomes. Although the process was widely studied, little is known about structure dynamics disordered histone tails, which play a pivotal role for nucleosome integrity. This gap filling experimental FRET study from perspective H3 N-terminal tail (H3NtT) reconstituted mononucleosomes. By systematic variation labeling positions we monitored motions H3NtT relative to dyad axis linker DNA. Single-molecule...
Neuroinflammation in the course of multiple sclerosis and experimental autoimmune encephalomyelitis results demyelination and, recently demonstrated, axonal loss. Invading neuroantigen specific T cells are crucial cellular elements these processes. Here we demonstrate that invasion activated induces a massive microglial attack on myelinated axons entorhinal‐hippocampal slice cultures. Flow cytometry analysis activation markers revealed state invading MBP‐specific was significantly lower...
Abstract The generation of antibody secretory cells from resting B lymphocytes after immunization with most protein Ag requires cell signaling by Ag, direct Th contact and lymphokines. Previous studies suggest that contact-mediated signals may be transduced Ia binding alpha beta TCR and/or CD4. Seemingly inconsistent this concept are findings cross-linking molecules on quiescent leads to cAMP is antagonistic for mitogenesis. Here we show ligand IL-4 receptors induce transition these into a...
Abstract Background Biting midges of the Obsoletus species complex ceratopogonid genus Culicoides were assumed to be major vectors bluetongue virus (BTV) in northern and central Europe during 2006 outbreak disease (BT). Most recently, field specimens same group have also been shown infected with newly emerged Schmallenberg (SBV) Europe. A reliable identification cryptic this is fundamental for both understanding epidemiology diseases targeted vector control. In absence classical...
Abstract Mice which bear the lpr gene spontaneously develop autoimmune syndromes characterized by massive expansion of an unusual T cell subset is phenotypically Thy‐1 + , L3T4 − Lyt‐2 B220 . The mutant cells are refractory to stimulation with mitogenic lectins and, implication, thought be solely responsible for defects in lymphokine production manifested mice. contribution remaining latter derangements has not been previously examined and focus this study. We found that abnormalities...
DNA compaction and accessibility in eukaryotes are governed by nucleosomes orchestrated through interactions between DNA-binding proteins. Using QuantAFM, a method for automated image analysis of atomic force microscopy (AFM) data, we performed detailed statistical structural properties mono-nucleosomes. QuantAFM allows fast AFM images, including preprocessing, object segmentation, quantification different parameters to assess nucleosomes. A comparison reconstituted with without linker...
Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) are biological vectors internationally important arboviruses. To understand role in transmission these viruses, it is essential to correctly identify species involved. Within western Palaearctic region, main suspected vector species, C. obsoletus, scoticus, dewulfi and chiopterus, have similar wing patterns, which makes difficult separate them correctly. In this study, designed as an inter-laboratory ring trial with twelve...
We have obtained cell lines producing monoclonal antibodies against μ, α, λ, and λ1 by fusing the mouse myeloma P3X63Ag8.653 with spleen cells from Lewis rats immunized several bearing proteins. The anti-λ1 antibody is particularly useful. It was shown to be suitable for radioimmunoassays, ELISAs when coupled alkaline phosphatase, indirect fluorescence staining of membrane as well cytoplasm.
Complete suppression of expression immunoglobulin kappa light chain was achieved by injecting female mice from birth with a mixture antisera against the mu heavy and (anti-mu anti-kappa). Then their offspring were injected anti-kappa birth. This resulted in stable as long injections continued. not detectable either serum or at cellular level. The number B cells spleen concentration classes subclasses normal. normal levels compensating enhancement lambda expression. Analysis chains...
Nucleosomes are important for chromatin compaction and gene regulation; their integrity depends crucially on the structural properties of histone tails. Recent all-atom molecular dynamics simulations revealed that removal N-terminal tails H3, known to destabilize nucleosomes, causes a rearrangement two arginines H2A, namely R81 R88 by altering electrostatic environment H2A α3 domain. Whether this is cause or effect decreased stability, unclear. Here, we emulate altered was found after H3...
The cis-acting gene regulating specifically the inducibility of lambda 1-bearing B cells has been mapped within 2.9 cM structural gene. If 1lo-phenotype is due to gly leads val interchange in C 1, then an argument can be made that (a) inefficient induction 1lo-bearing and (b) cell triggering dependent upon a conformational change Ig receptor interaction with antigen. regulatory sequence linked 1-gene, it must control expression 1-locus during development into adulthood, e.g., by effect on...
Abstract T cells from murine lupus strains manifest complex defects in interleukin 2 (IL 2) production and receptor expression. The capacity of B such mice to utilize IL2 as a growth factor has not been previously reported is examined herein. Anti‐Thy‐1.2 plus complement‐treated spleen 6–8‐week‐old autoimmune MRL‐ Ipr/lpr age sex‐matched immunologically normal CBA/J were cultured with lipopolysaccharide (LPS) for 36 h analyzed the expression IL receptors using monoclonal antibody 7D4....
We report on the substitution of silver nanoparticles' inks by microparticle dispersions as a material for production printable tracks laser melting. This approach is promising, because it helps to reduce costs such tracks. Though used materials melting mostly contain polyvinylpyrrolidone stabilizer, which results in appearance an undesired balling effect during melting, authors test stabilizers differing molecular weight and functionality. The resulting differences colloidal physicochemical...
Viral infections can lead to platelet activation and hemostatic complications. However, the extent which reactivity remains altered after convalescence, contributing long-term health impairments as observed COVID-19 is not yet fully understood. Therefore, we conducted a cohort study (DRKS00025217) determine function in individuals convalesced from mild COVID-19. Assays were performed ex vivo with blood convalescents at 2–15 weeks 6–10 months focusing on aggregation, markers, thrombin...
Abstract A hybridoma from the λ‐defective mouse strain SJA has been established. It produces a λ1‐bearing IgG 2b , dextran B 1355‐binding antibody. The DNA sequence of VJ and C gene segment was in complete accordance with published germ‐line sequence. rate secretion steady state level cytoplasmic RNA this line comparable to that cell lines normal mice. idiotype closely related MOPC 104E indicating λ1 light chain is associated same V H region similar fashion as BALB/c This antibody should be...