A5077416916- thermodynamics and calorimetric analyses
- Chemical Thermodynamics and Molecular Structure
- Phase Equilibria and Thermodynamics
- DNA and Nucleic Acid Chemistry
- Protein Structure and Dynamics
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Machine Learning in Materials Science
- Cancer therapeutics and mechanisms
- Heat shock proteins research
- Bacterial Genetics and Biotechnology
- Lipid Membrane Structure and Behavior
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Metal complexes synthesis and properties
- Crystallization and Solubility Studies
- HIV Research and Treatment
- Computational Drug Discovery Methods
- Chemical and Physical Properties in Aqueous Solutions
- Thermal and Kinetic Analysis
- Mass Spectrometry Techniques and Applications
- Catalysis and Oxidation Reactions
- HIV/AIDS drug development and treatment
- Bacterial Infections and Vaccines
Rutgers, The State University of New Jersey
2009-2024
Stony Brook University
2003-2011
Rutgers Sexual and Reproductive Health and Rights
2009
Rütgers (Germany)
2009
National Institutes of Health
1995-2002
National Heart Lung and Blood Institute
1997-2002
National Cancer Institute
2002
University of California, Berkeley
1999
Biocom
1999
Johns Hopkins University
1998-1999
We present a comparative study of calorimetrically derived thermodynamic profiles for the binding series drugs with selected DNA host duplexes. use these data to demonstrate that comparisons between complete (delta G zero, delta H S Cp) are required before drug can be used as probe conformation, since enthalpy-entropy compensations cause two drug-DNA events exhibit similar free energies zero) despite being driven by entirely different forces zero). In this work, we employ combination...
α-Synuclein (αS) is an amyloidogenic intrinsically disordered protein implicated in Parkinson's disease, for which copper-mediated pathways of neurodegeneration have been suggested. We employed nuclear magnetic resonance, circular dichroism, electrospray ionization mass spectrometry, and thioflavin T fluorescence to characterize interactions Cu(2+) with the physiological acetylated form (Ac-αS). Significantly, N-terminal acetylation abolishes binding at high-affinity M1-D2 site present...
Batch calorimetry, differential scanning calorimetry (DSC), uv/vis absorption spectroscopy, fluorescence and circular dichroism (CD), have been used to detect, monitor, thermodynamically characterize the binding of daunomycin, dipyrandenium, dipyrandium, netropsin poly d(AT) actinomycin D salmon testes (ST) DNA. The following thermodynamic profiles obtained. (table; see text) All studies were done at 25 degrees C while ST DNA was performed 1 degree enhance drug solubility. These parameters...
Abstract The energetics of structural changes in the holo and apo forms α‐lactalbumin transition between their native denatured states induced by binding Ca 2+ Na + have been studied differential scanning isothermal titration microcalorimetry circular dichroism spectroscopy under various solvent conditions. Removal from protein enhances its sensitivity to pH ionic conditions due noncompensated negative charge‐charge interactions at cation site, which significantly reduces overall stability....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThermodynamic characterization of daunomycin-DNA interactions: Comparison complete binding profiles for a series DNA host duplexesDavid P. Remeta, Courtney Mudd, Robert L. Berger, and Kenneth J. BreslauerCite this: Biochemistry 1993, 32, 19, 5064–5073Publication Date (Print):May 18, 1993Publication History Published online1 May 2002Published inissue 18...
We have employed a combination of temperature-dependent UV absorption spectroscopy, circular dichroism, and batch calorimetry to characterize the binding actinomycin D series oligomeric DNA duplexes. find duplex [d(CGTCGACG)]2 be unique in its ability bind strongly despite absence classic GpC site. present evidence that this non-GpC-containing binds two molecules an apparently cooperative manner form complex exhibits aberrant spectroscopic calorimetric behavior. propose these observations...
The targeting of type III secretion (TTS) proteins at the injectisome is an important process in bacterial virulence. Nevertheless, how specifically recognizes TTS substrates among all unknown. A peripheral membrane ATPase protein located base has been implicated process. We have investigated EspA filament and its cognate chaperone, CesAB, to EscN enteropathogenic E. coli (EPEC). show that selectively engages EspA-loaded CesAB but not unliganded CesAB. Structure analysis revealed signal...
An outer membrane PorB class 2 protein fromNeisseria meningitidis has been overexpressed inEscherichia coli, isolated from inclusion bodies, and refolded in the presence of zwitterionic detergent. The purified recombinant native (strain M986) counterpart exhibit most typical functional structural properties that are characteristic bacterial porins. Channel forming activity monitored by incorporating into reconstituted liposomes measuring permeation rates various oligosaccharides through...
A genetically detoxified pneumolysin, pneumolysoid (PLD), was investigated as a carrier protein for pneumococcal capsular polysaccharide (CPS). Such CPS-PLD conjugate might provide additional protection against infections and resultant tissue damage. single point mutant of pneumolysin selected, which lacked measurable haemolytic activity, but exhibited the overall structural immunological properties wild type. PLD conjugates were prepared from CPS serotypes 6B, 14, 19F, 23F by reductive...
We used stopped-flow calorimetry to measure the overall enthalpy change associated with template-directed nucleotide insertion and DNA extension. Specifically, we families of hairpin self-priming templates in conjunction an exonuclease-free polymerase study primer extension by one or more dA dT residues. Our results reveal exothermic heats between –9.8 –16.0 kcal/bp for enzymatic polymerization. These enthalpies depend on identity inserting base, terminus, and/or preceding base. Despite...
DNA bulges are biologically consequential defects that can arise from template-primer misalignments during replication and pose challenges to the cellular repair machinery. Calorimetric spectroscopic characterizations of defect-containing duplexes reveal systematic patterns sequence-context dependent bulge-induced destabilizations. These distinguishing energetic signatures manifest in three coupled characteristics, namely: magnitude duplex destabilization (ΔΔGBulge); thermodynamic origins...
Accumulation of damaged guanine nucleobases within genomic DNA, including the imidazole ring opened N 6 -(2-Deoxy-α,β-D- erythro -pentafuranosyl)-2,6-diamino-4-hydroxy-5-formylamidopyrimidine (Fapy-dG), is associated with progression age-related diseases and cancer. To evaluate impact this mutagenic lesion on DNA structure energetics, we have developed a novel synthetic strategy to incorporate cognate Fapy-dG site-specifically any oligodeoxynucleotide sequence. The scheme involves synthesis...
PorB proteins constitute the vast majority of channels in neisserial outer membranes and can be subdivided within meningococcal strains into two distinct mutually exclusive families that are designated as class 2 3 proteins. We recently characterized functional activity conformational stability a protein from <i>Neisseria meningitidis</i> (Minetti, C. A. S. A., Tai, J. Y., Blake, M. S., Pullen, K., Liang, M., Remeta, D. P. (1997)<i>J. Biol. Chem.</i> 272, 10710–10720). To evaluate...
We report a general method for screening, in solution, the impact of deviations from canonical Watson-Crick composition on thermodynamic stability nucleic acid duplexes. demonstrate how fluorescence resonance energy transfer (FRET) can be used to detect directly free differences between an initially formed “reference” duplex (usually duplex) and related “test” containing lesion/alteration interest (e.g., mismatch, modified, deleted, or bulged base, etc.). In one application, titrates into...
The conformational and thermal stability of full-length hemagglutinin (HA) influenza virus (strain X31) has been investigated using a combination differential scanning calorimetry (DSC), analytical ultracentrifugation, fluorescence, circular dichroism (CD) spectroscopy as function pH. HA sediments rosette comprised 5-6 trimers (31-35 S) over the pH range 7.4-5.4. DSC profile in native state at 7.4 is characterized by single cooperative endotherm with transition temperature (Tm) 66 degrees C...
Abstract Acrolein is an α,β‐unsaturated aldehyde that a major environmental pollutant, as well product of cellular metabolism. DNA bases react with acrolein to form two regioisomeric exocyclic guanine adducts, namely γ‐hydroxy‐propanodeoxyguanosine (γ‐OH‐PdG) and its positional isomer α‐hydroxy‐propanodeoxyguanosine (α‐OH‐PdG). The γ‐OH‐PdG adopts ring‐opened conformation minimal structural perturbation the host duplex. Conversely, α‐OH‐PdG assumes ring‐closed significantly disrupts...
ThermoML is an XML-based approach for storage and exchange of experimental, predicted, critically evaluated thermophysical thermochemical property data. Extensions to the schema representation speciation, complex equilibria, properties biomaterials are described. The texts 14 data files illustrating new extensions provided as Supplementary Information together with complete text updated schema.