A5003808755- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sexual Differentiation and Disorders
- Genetic Mapping and Diversity in Plants and Animals
- Chromosomal and Genetic Variations
- Genetic and phenotypic traits in livestock
- Genomics and Chromatin Dynamics
- Urological Disorders and Treatments
- Genomics and Phylogenetic Studies
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Genomic variations and chromosomal abnormalities
- MicroRNA in disease regulation
- Human-Animal Interaction Studies
- Reproductive Biology and Fertility
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Peroxisome Proliferator-Activated Receptors
- Nuclear Structure and Function
- Agriculture, Plant Science, Crop Management
- Virus-based gene therapy research
- Congenital Anomalies and Fetal Surgery
- Genetic diversity and population structure
University of Life Sciences in Poznań
2016-2025
Institute of Plant Genetics, Polish Academy of Sciences
2019-2024
MRC Weatherall Institute of Molecular Medicine
2017-2018
University of Life Sciences in Lublin
2013-2018
University of Oxford
2018
University of Cincinnati Medical Center
2017
Instytut Środowiska Rolniczego i Leśnego Polskiej Akademii Nauk
2005-2007
University of Agriculture in Krakow
2005
Abstract Xenotransplantation from pigs could alleviate the shortage of human tissues and organs for transplantation. Means have been identified to overcome hyperacute rejection acute vascular mechanisms mounted by recipient. The challenge is combine multiple genetic modifications enable normal animal breeding meet demand transplants. We used two methods colocate xenoprotective transgenes at one locus, sequential targeted transgene placement - ‘gene stacking’ cointegration engineered large...
Abstract Self-interacting chromatin domains encompass genes and their cis -regulatory elements; however, the three-dimensional form a domain takes, whether this relies on enhancer–promoter interactions, processes necessary to mediate formation maintenance of such domains, remain unclear. To examine these questions, here we use combination high-resolution chromosome conformation capture, non-denaturing fluorescence in situ hybridisation super-resolution imaging study 70 kb encompassing mouse...
Congenital malformations resulting in late abortions and stillbirths affect the economic wellbeing of producers welfare cattle breeding programs. An extremely high incidence "half-sized" calves normal karyotype uninflated lungs was diagnosed progeny Finnish Ayrshire (Bos taurus) bull - YN51. No other visible anatomical abnormalities were apparent stillborn calves. We herein describe positional identification a 110 kb microdeletion maternally imprinted PEG3 domain that results loss paternal...
Studies in cultured cells have demonstrated the existence of higher-order epigenetic mechanisms, determining relationship between expression gene and its position within cell nucleus. It is unknown, whether such mechanisms operate postmitotic, highly differentiated types, as neurons vivo. Accordingly, we examined intranuclear positions Bdnf Trkb genes, encoding major neurotrophin receptor respectively, change a result neuronal activity, what functional consequences movements may have. In rat...
Abstract The pig oocyte maturation protocol differs from other mammalian species due to dependence on follicular fluid (FF) supplementation. One of the most abundant components porcine are fatty acids (FAs). Although evidence models revealed a negative impact saturated (SFA) developmental competence oocytes, has not yet been widely analyzed. Therefore, we aimed investigate whether supplementation IVM medium with 150 μM stearic acid (SA) and oleic (OA) affects lipid content expression genes...
Hypospadias is rarely reported in dogs. In this study we pre-sent 2 novel cases of disorder sexual development and, addition, a case hereditary sex reversal female with an enlarged clitoris. The first was male Moscow watchdog normal karyotype (78,XY) and the presence <i>SRY</i> gene. dog, perineal hypospadias, bilateral inguinal cryptorchidism testes were observed. second case, representing Cocker spaniel breed, had small penis hypospadic orifice urethra, cryptorchidism, testis...
Peruvian maca (Lepidium meyenii) is a plant known for its nutritional and medicinal properties whose use as supplement in animal diets has attracted much interest. We studied the effects of powdered root extract on growth potential vitro cultured porcine cells prior to an additive nutrition. Fibroblast cell viability (MTT), proliferation (BrdU), apoptosis level (TUNEL) were measured range doses (0, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 7.0, 10 mg/mL). Transcript levels CCND1, MCM2, PCNA genes...
Impaired migration of primordial germ cells during embryonic development causes hereditary gonadal hypoplasia in both sexes Northern Finncattle and Swedish Mountain cattle. The affected gonads exhibit a lack or, rare cases, reduced number cells. Most animals present left-sided hypoplasia. However, right-sided bilateral cases are also found. This type prevails with white coat colour. Previous studies indicated that is inherited an autosomal recessive fashion incomplete penetrance. In order to...
Dietary triggers acting on a developing fetus can affect the functioning of body in later life; this be observed various levels, including epigenetic modifications and gene expression. Early-life programmed changes may transmitted to successive generations. In study, impact prenatal restricted diet was studied four generations rats. We hypothesized that induce expression major genes involved two mechanisms: DNA methylation histone modifications. The transcript level six these processes...
Abstract Although the disorder of sex development in dogs with female karyotype (XX DSD) is quite common, its molecular basis still unclear. Among mutations underlying XX DSD mammals are duplication a long sequence upstream SOX9 gene (RevSex) and (also observed dogs). We performed comparative analysis 16 30 control dogs, using FISH MLPA approaches. Our study was focused on region harboring orthologous to human RevSex (CanRevSex), which located by silico downstream . Two highly polymorphic...
SRY-positive XX testicular disorder of sex development (DSD) caused by X;Y translocations was not yet reported in domestic animals. In humans it is rarely diagnosed and a majority clinical features resemble those which are typical for Klinefelter syndrome (KS). Here we describe the first case DSD tortoiseshell cat with rudimentary penis lack scrotum.Molecular analysis showed presence two Y-linked genes (SRY ZFY) normal sequence SRY gene. Application classical cytogenetic techniques revealed...
Proper expression of the PPARG gene, which encodes a key transcription factor adipogenesis, is indispensable in formation mature adipocytes. The positioning gene within nuclear space has been implicated regulation. We here report on significance gene’s for its activity during vitro adipogenesis pig. used an established system differentiation mesenchymal stem cells derived from bone marrow and adipose tissue into process was carried out 7 days, were examined using 3D DNA/immuno-FISH...
Testicular or ovotesticular disorders of sex development (DSD) in individuals with female karyotype (XX) lacking the SRY gene has been observed several mammalian species, including dogs. A genetic background for this abnormality extensively sought, and region harboring SOX9 often considered key canine DSD. Three types polymorphism have studied to date: a) copy number variation (CNV) a about 400 kb upstream SOX9, named CNVR1; b) duplication SOX9; c) insertion single G-nucleotide (rs852549625)...
Abstract A characteristic spatial distribution of the main chromatin fractions is observed in most mammalian cell nuclei, with euchromatin localized interior and heterochromatin at nuclear periphery. It has been shown that interactions lamina are necessary to establish this conventional architecture. Adipocytes specific cells which a reduction lamin A/C expression observed. We hypothesize loss during adipogenic differentiation mesenchymal stem (MSCs) may be associated reorganization classes...