A5008275706- DNA Repair Mechanisms
- PARP inhibition in cancer therapy
- Osteoarthritis Treatment and Mechanisms
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Skin Protection and Aging
- melanin and skin pigmentation
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Skin and Cellular Biology Research
- Inflammatory Bowel Disease
- Microscopic Colitis
- Dermatologic Treatments and Research
- Cell Adhesion Molecules Research
- Integrated Circuits and Semiconductor Failure Analysis
- Mesenchymal stem cell research
- Dermatological and Skeletal Disorders
- Cancer-related gene regulation
- Epoxy Resin Curing Processes
- CRISPR and Genetic Engineering
- Celiac Disease Research and Management
- Proteoglycans and glycosaminoglycans research
- Calcium Carbonate Crystallization and Inhibition
- Frailty in Older Adults
Inserm
2013-2025
Université de Rennes
2013-2025
Centre de Recherche Épidémiologie et Statistique
2024-2025
Institut de génétique et de développement de Rennes
2023-2025
Centre National de la Recherche Scientifique
2023-2025
University of Pittsburgh
2024
Université Paris Cité
2024
Hôpital Saint-Louis
2024
Assistance Publique – Hôpitaux de Paris
2024
Université de Lyon
2023
Idecabtagene vicleucel (ide-cel), a chimeric antigen receptor T-cell therapy targeting B-cell maturation (BCMA), received early access program (EAP) authorization in France April 2021 for relapsed/refractory multiple myeloma (RRMM). We conducted real-world registry-based multicentre observational study 11 French hospitals to evaluate ide-cel outcomes. Data from 176 RRMM patients who underwent apheresis between June and November 2022 were collected the national DESCAR-T registry. Of these,...
Coordination of the cellular response to DNA damage is organised by multi-domain ‘scaffold’ proteins, including 53BP1 and TOPBP1, which recognise post-translational modifications such as phosphorylation, methylation ubiquitylation on other are themselves carriers regulatory signals. Here we show that checkpoint regulating S-phase entry controlled a phosphorylation-dependent interaction TOPBP1. BRCT domains TOPBP1 selectively bind conserved phosphorylation sites in N-terminus 53BP1. Mutation...
Abstract Long-term cultures under hypoxic conditions have been demonstrated to maintain the phenotype of mesenchymal stromal/stem cells (MSCs) and prevent emergence senescence. According several studies, hypoxia has frequently reported drive genomic instability in cancer MSCs by hindering DNA damage response repair. Thus, we evaluated occurrence repair events during ex vivo expansion clinical-grade adipose-derived stromal (ADSCs) bone marrow (BM)-derived cultured with platelet lysate 21%...
ADP-ribosylation signaling orchestrates the recruitment of various repair actors and chromatin remodeling processes promoting access to lesions during early stages DNA damage response. The remodeler complex ACF, composed ATPase subunit SMARCA5/SNF2H cofactor ACF1/BAZ1A, is among factors that accumulate at in an dependent manner. In this work, we show each ACF accumulates breaks independently from its partner. Furthermore, demonstrate SMARCA5 ACF1 sites not due direct binding ADP-ribose...
Abstract Background Crohn’s disease (CD) is a chronic and progressive condition with growing global prevalence, leading to potential bowel damage disability. Data on the clinical presentation, outcomes, treatment options in newly diagnosed CD patients Europe current era are essential guide physicians. The aim describe characteristics of contemporary European cohort recently patients. Methods Crohn's Disease Cohort (CROCO) an ongoing, multicenter, prospective study within last 12 months,...
Abstract Background Crohn’s disease (CD) often results in progressive bowel damage and disability. Disability has been proposed as an endpoint for disease-modification trials by the SPIRIT-IOIBD1 consensus, though data on evolution of IBD-Disability Index (IBD-DI) over time remain limited. Methods The Disease Cohort (CROCO) is a prospective, ongoing study newly diagnosed CD patients (within 12 months diagnosis), assessing changes was measured using validated (IBD-DI), which includes 14...
Abstract Background Crohn’s disease (CD) progression manifests as bowel damage (BD) and disability; prevention of both is a long-term treatment target in CD. However, the longitudinal characterization BD disability early CD patients remains limited. Methods The Crohn´s Disease Cohort (CROCO) multicentre, European cohort study recently diagnosed (<12 months following diagnosis) designed to prospectively characterize disability. At one year diagnosis (Y1), was evaluated using Lemann...
RING‐type E3 ubiquitin ligases promote ubiquitylation by stabilising an active complex between a ubiquitin‐loaded E2‐conjugating enzyme and protein substrate. To fulfil this function, the ubiquitin‐protein ligase SIAH1 other SINA/SIAH subfamily employ N‐terminal catalytic RING domain C‐terminal substrate‐binding (SBD), separated two zinc fingers. Here, we present first crystal structure of human SIAH1, together with adjacent finger, revealing potential dimer, which was validated in solution...
A significant association between a polymorphism in the D repeat of gene encoding asporin and osteoarthritis, most frequent articular diseases, has been recently reported. The goal present study was to investigate expression this new class I small leucine-rich proteoglycan (SLRP) human chondrocytes. First, we studied modulation (ASPN) by cytokines Western blot reverse transcription-polymerase chain reaction. Interleukin-1β tumor necrosis factorβ downregulated ASPN, whereas transforming...
Mollusc shells are composed of more than 95% calcium carbonate and less 5% an organic matrix consisting mostly proteins, glycoproteins polysaccharides. Previous studies have elucidated the biological activities shell matrices from bivalve molluscs on skin, especially expression extracellular components fibroblasts. In this work, we investigated potential extracted scallop Pecten maximus human fibroblasts in primary culture. Firstly, demonstrated that had different effects general cellular...
The nuclear factor‐κB (NF‐κB) pathway is a key mediator of inflammation; however, few studies have examined the direct effects NF‐κB inhibition on skin. To investigate activity in cultured human fibroblasts and to 4‐hexyl‐1,3‐phenylenediol (an inhibitor) elastin collagen gene expression vitro clinical appearance photodamaged amount obtained from donors (17–78 years old) was measured after transfection with reporter luciferase promoter system. extracellular matrix (ECM) genes determined using...
The INhibitor of Growth (ING) proteins are encoded as multiple isoforms in five ING genes (ING1 -5) and act type II tumor suppressors. They growth inhibitory when overexpressed frequently mislocalized or downregulated several forms cancer. ING1 ING2 stoichiometric members histone deacetylase complexes, whereas ING3-5 components different acetyltransferase complexes. INGs target these complexes to marks, thus acting epigenetic regulators. affect angiogenesis, apoptosis, DNA repair, metastasis...
Abstract Hypoxic niches help maintain mesenchymal stromal cell properties, and their amplification under hypoxia sustains immature state. However, how MSCs genomic integrity in this context remains elusive, since may prevent proper DNA repair by downregulating expression of BRCA1 RAD51. Here, we find that the ING1b tumor suppressor accumulates adipose-derived cells (ADSCs) upon genotoxic stress, owing to SUMOylation on K193 is mediated E3 small ubiquitin-like modifier (SUMO) ligase protein...
Human epidermal keratinocytes and dermal fibroblasts were co-cultured on polysulfone (PSU) membranes, previously coated or not with hyaluronan (HA), compared to monocultured fibroblasts. The purpose was define the interplay between both cell types how it is influenced. co-cultures reduced I III collagen levels, indicating that exerted an inhibition matrix synthesis by On other hand, amounts of keratins 17 10 increased, suggesting stimulate production keratinocytes. In contrast naked PSU HA...
Abstract Background Crohn’s disease (CD) can lead to progressive bowel damage and disability. Disability has been proposed by the SPIRIT-IOIBD consensus as an endpoint in disease-modification trials. Despite this, there is scarce data on disability at CD diagnosis. Methods The Crohn´s Disease Cohort (CROCO) ongoing prospective cohort study with newly diagnosed patients (within 12 months of diagnosis) aiming assess evolution over time. evaluation was conducted via validated IBD-Disability...