A5044757320- Cervical Cancer and HPV Research
- Genital Health and Disease
- Reproductive tract infections research
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Global Cancer Incidence and Screening
- Cancer-related molecular mechanisms research
- Urological Disorders and Treatments
- Urinary and Genital Oncology Studies
- RNA modifications and cancer
- Head and Neck Cancer Studies
- Colorectal Cancer Screening and Detection
- Colorectal and Anal Carcinomas
- Cell death mechanisms and regulation
- Lymphoma Diagnosis and Treatment
- AI in cancer detection
- Congenital Diaphragmatic Hernia Studies
- Hepatitis B Virus Studies
- Parvovirus B19 Infection Studies
- Chronic Lymphocytic Leukemia Research
- Cancer Risks and Factors
- Animal Virus Infections Studies
- Esophageal Cancer Research and Treatment
- Animal Disease Management and Epidemiology
Vrije Universiteit Amsterdam
2016
Amsterdam UMC Location Vrije Universiteit Amsterdam
2006-2015
Wageningen University & Research
2005
Abstract Human papillomavirus type 16 (HPV16) plays a role in the development of subgroup head and neck squamous cell carcinomas (HNSCC). However, uncertainty exists about true impact HPV this tumor as conflicting reports have been published with prevalence rates from 0 to 100%. We aimed find detection algorithm biologically thus clinically meaningful infection, applicable for high‐throughput screening frozen formalin‐fixed paraffin embedded (FFPE) specimens. By considering E6 oncogene...
Given the strong etiologic link between high-risk HPV infection and cervical cancer testing is now being considered as an alternative for cytology-based screening. Many test systems have been developed that can detect broad spectrum of hrHPV types in one assay. However, screening purposes detection not inherently useful unless it informative presence high-grade intraepithelial neoplasia (CIN 2/3) or cancer. Candidate tests to be used should reach optimal balance clinical sensitivity...
Abstract High‐risk human papillomavirus (hrHPV) testing has a higher sensitivity but lower specificity than cytology for detection of high‐grade intraepithelial neoplasia (CIN). To avoid over‐referral to colposcopy and overtreatment, hrHPV‐positive women require triage and/or followup. A total 25,658 (30–60 years) enrolled in population‐based cohort study had an adequate baseline Pap smear hrHPV test. The end‐point was cumulative two‐year risk CIN grade 3 or worse (CIN3+). In post‐hoc...
Abstract Human papillomavirus (HPV) infection has been etiologically linked to oropharyngeal squamous cell carcinoma (OPSCC). The prevalence of HPV‐positive OPSCC varies between studies, ranging from 20 90%. This may be related the lack a standardized HPV detection assay as well time period in which is investigated, rising incidence rates are reported over last decades. Here, we validated our previously defined test algorithm for formalin‐fixed paraffin‐embedded (FFPE) tumor specimen...
This study shows that the clinical performance and reproducibility of cobas 4800 HPV test for high-risk human papillomavirus (HPV) detection fulfill criteria as formulated in international guidelines requirements cervical screening purposes. Accordingly, can be considered clinically validated screening.
Screening women for high-grade cervical intraepithelial neoplasia or cancer (CIN3(+)) by high-risk human papillomavirus (hrHPV) testing has as side-effect the detection of hrHPV-positive without clinically relevant lesions. Here, we developed an objective assay assessing methylation status promoter regions CADM1 and MAL to triage CIN3(+).In a training set (51 with CIN3(+) 224 CIN2(+)), panels consisting one four quantitative methylation-specific PCR (qMSP) assays...
Recent studies have reported that p16 protein overexpression qualifies as a surrogate marker identifying an oncogenic human papillomavirus (HPV) infection in oropharyngeal squamous cell carcinoma (OPSCC). However, there is still percentage of OPSCCs are positive for immunohistochemistry (p16 IHC) but lack HPV DNA. The objective this study was to characterize group at the molecular level by performing sensitive DNA- and RNA-based PCR methods genetic profiling. All patients diagnosed with...
Abstract Primary testing for human papillomavirus (HPV) in cervical screening requires triage to differentiate women with transient infection from those persistent who require more intensive management given their risk (pre)cancer. In this study, the clinical performance of a novel methylation marker FAM19A4 high-risk (hr)HPV-positive was evaluated. Using training-validation set approach, we analyzed quantitative methylation-specific PCR (qMSP). The training comprised hrHPV-positive scrapes...
Combined detection of cell adhesion molecule 1 (CADM1) and T-lymphocyte maturation-associated protein (MAL) promoter methylation in cervical scrapes is a promising triage strategy for high-risk human papillomavirus (hrHPV)-positive women. Here, CADM1 MAL DNA levels were analysed hrHPV-positive women with no underlying high-grade disease, intraepithelial neoplasia (CIN) cancer. first related to CIN-grade the corresponding biopsy second stratified by presence 'normal' or 'abnormal' cytology as...
DNA methylation analysis of cancer-related genes is a promising tool for HPV-positive women to identify those with cervical (pre)cancer (CIN3+) in need treatment. However, clinical performance markers can be influenced by the sample type utilized. We describe multiplex quantitative methylation-specific PCR that targets FAM19A4 and mir124-2 loci, detect CIN3+ using both lavage- brush self-samples.We determined thresholds classification training sets comprising lavage self-samples 182...
Abstract In human papillomavirus (HPV) cervical cancer screening, cytology is used as triage to counter the low specificity of HPV testing. VALID‐SCREEN a EU‐multicenter, retrospective study conducted evaluate clinical performance FAM19A4/miR124‐2 methylation‐based molecular test substitute or addition reflex testing screen positive women. methylation (QIAsure Methylation Test) was evaluated in 2384 HPV‐positive screening samples, from women 29‐76 years age, derived four EU countries....
Background. Genital human papillomavirus (HPV) infections are generally accepted to be sexually transmitted, but studies of HPV in sex partners limited. We investigated type-specific concordance and viral load 238 heterosexual couples. Women with cervical intraepithelial neoplasia were the index patients these
An increased high-risk human papillomavirus (hrHPV) viral load in cervical scrapings has been proposed as a determinant for high-grade intraepithelial neoplasia (CIN) and cancer (> or =CIN 2), but data so far HPV types different from 16 are limited inconsistent. In addition, threshold to distinguish hrHPV positive women without > 2 still not defined. Here, we used baseline of with normal cytology participating large population-based screening trial (i.e. POBASCAM) who were GP5+/6+-PCR 4...
Given the lower specificity for high-grade cervical lesions of high-risk human papillomavirus (hrHPV) testing compared to cytology, additional triage hrHPV test-positive women is needed detect lesions. Here, we tested whether combined methylation analysis cell adhesion molecule 1 (CADM1) and T-lymphocyte maturation associated protein (MAL), both functionally involved in carcinogenesis, could serve as such a marker. Four quantitative methylation-specific PCRs (qMSP), two CADM1 (regions M12...
Aims Gene promoter hypermethylation is recognised as an essential early step in carcinogenesis, indicating important application areas for DNA methylation analysis cancer detection. The current study was set out to assess the performance of CADM1 , MAL and miR124- 2 cervical scrapes detection endometrial cancer. Methods A series women with (n=79) or (n=21) cancer, intraepithelial neoplasia grade 3 (CIN3) (n=16) CIN2 (n=32), without evidence worse (n=120) were assessed miR124-2 . Methylation...
Widespread adoption of primary human papillomavirus (HPV)‐based screening has encouraged the search for a triage test which retains high sensitivity detection cervical cancer and precancer, but increases specificity to avoid overtreatment. Methylation analysis FAM19A4 miR124‐2 genes shown promise high‐risk (hr) HPV‐positive women. In our study, we assessed consistency / methylation in series 519 invasive carcinomas ( n = 314 scrapes, 205 tissue specimens) from over 25 countries, using...
ABSTRACT A user-friendly self-sampling method for collecting representative cervical cell material would lower the threshold women to respond invitation screening. In present article, we introduce such a device; have evaluated its sensitivity and specificity detect high-grade intraepithelial neoplasia (CIN), via high-risk human papillomavirus (hrHPV) detection liquid-based cytology (LBC), compared endocervical brush samples obtained by gynecologists. Women who had smear reading of moderate...
ABSTRACT The HPV-Risk assay is a novel real-time PCR targeting the E7 region of 15 high-risk human papillomavirus (HPV) types (i.e., HPV16, -18, -31, -33, -35, -39, -45, -51, -52, -56, -58, -59, -66, -67, and −68), provides additional genotype information for HPV16 HPV18. This study evaluated clinical performance reproducibility with cervical scraping specimens its utility self-collected (cervico)vaginal specimens. intraepithelial neoplasia grade 2 or worse (CIN2+) was by noninferiority...
ABSTRACT The Aptima HPV assay (Hologic Gen-Probe, San Diego, CA) is an FDA-approved for detecting human papillomavirus (HPV) E6/E7 mRNA from 14 high-risk types. This study evaluated the clinical performance of cervical intraepithelial neoplasia grade 2 or worse (CIN2+), relative to GP5+/GP6+ PCR, in a cross-sectional equivalence analysis using noninferiority score test with samples population-based screening, i.e., 69 scraping women CIN2+ and 843 without evidence CIN2+. In addition,...
Quantitative methylation-specific PCR (qMSP) analysis for determining the methylation status of (candidate) tumor suppressor genes has potential as objective and valuable test to triage high-risk human papillomavirus (hrHPV) positive women in cervical screening. Particularly combined a panel shows most promising clinical performance, with sensitivity levels that equal or exceed cytology. However, wide application such marker panels is hampered by lack effective multiplex assays allowing...
Methylation markers were studied for their suitability to triage human papillomavirus (HPV)-positive women by testing self-collected cervico-vaginal lavage specimens. For this purpose, we analyzed 355 hrHPV-positive specimens with three methylation markers, that is, CADM1-m18, MAL-m1 and miR-124-2 quantitative methylation-specific PCR. The areas under the receiver-operating characteristic (ROC) curve end-point cervical intraepithelial neoplasia grade 3 or worse (CIN3+) 0.637 0.767 0.762...
Anyplex™ II HPV HR Detection (Seegene, Seoul, Korea) is a multiplex real-time PCR using tagging oligonucleotide cleavage and extension (TOCE) technology for simultaneous detection genotyping of 14 high-risk (HR) types, including HPV16 HPV18.To evaluate whether the clinical performance reproducibility meet international consensus guidelines test requirements cervical cancer screening [1].The detecting intraepithelial neoplasia grade 2 or worse (CIN2+) was determined relative to that reference...
Abstract In a population‐based cervical screening cohort, we determined the value of type‐specific viral load assessment for detection high‐grade intraepithelial neoplasia and cancer (≥CIN2). Viral was by real‐time PCR in women with single HPV16,‐18,‐31 ‐33 infections, as GP5+/6+‐PCR. Study endpoints were cumulative ≥CIN2 or ≥CIN3 within 18 months follow‐up. High loads HPV16,‐31, predictive (relative risk 1.6 (95% CI: 1.3–1.9), 1.7 1.1–2.7) 1.9 1.1–3.1) per 10‐fold change load,...