A5077650743- Cervical Cancer and HPV Research
- Gestational Trophoblastic Disease Studies
- Prenatal Screening and Diagnostics
- Endometrial and Cervical Cancer Treatments
- Ectopic Pregnancy Diagnosis and Management
- Cancer-related molecular mechanisms research
- Ovarian cancer diagnosis and treatment
- Cancer Risks and Factors
- Epigenetics and DNA Methylation
- Global Cancer Incidence and Screening
- Endometriosis Research and Treatment
- Orthopedic Surgery and Rehabilitation
- Reproductive tract infections research
- MicroRNA in disease regulation
- Wound Healing and Treatments
- Burn Injury Management and Outcomes
- Uterine Myomas and Treatments
- Genital Health and Disease
- Cancer Genomics and Diagnostics
- Urinary and Genital Oncology Studies
- Reproductive Biology and Fertility
- Bladder and Urothelial Cancer Treatments
- Peripheral Nerve Disorders
- Elbow and Forearm Trauma Treatment
- Pressure Ulcer Prevention and Management
The Netherlands Cancer Institute
2018-2025
Centrum voor Gynaecologische Oncologie Amsterdam
2015-2025
Amsterdam University of Applied Sciences
2023
Norwegian Womens Public Health Association
2023
Association of Dutch Burn Centres
2020
Gynecologic Oncology Group
2019
Radboud University Medical Center
2004-2015
Radboud University Nijmegen
2004-2015
Canisius-Wilhelmina Ziekenhuis
2013
Amsterdam UMC Location University of Amsterdam
1999-2002
PD-1/PD-L1 immune checkpoint inhibitors show potential for cervical cancer treatment. However, low response rates suggest that patient selection based on PD-L1 protein expression is not optimal. Here, we evaluated different detection methods and studied transcriptional regulation of PD-L1/PD-L2 by The Cancer Genome Atlas (TCGA) mRNAseq analysis. First, determined the copy number locus fluorescence in situ hybridization (FISH), mRNA RNA (RNAish), immunohistochemistry (IHC) tissue microarrays...
Cervical screening can prevent cancer by detection and treatment of cervical intraepithelial neoplasia grade 2 or 3 (CIN2/3). Screening also results in considerable overtreatment because many CIN2/3 lesions show spontaneous regression when left untreated. In this multicenter longitudinal cohort study women with untreated CIN2/3, the prognostic value FAM19A4/miR124-2 methylation was evaluated for clinical regression.Women were prospectively followed 24 months. Surgical excision replaced a...
Abstract Purpose: Biomarker detection in urine offers a potential solution to increase effectiveness of cervical cancer screening programs by attracting nonresponders. In this prospective study, the presence high-risk human papillomavirus (hrHPV) DNA and performance methylation analysis was determined for high-grade intraepithelial neoplasia (CIN2/3) urine, compared with paired cervicovaginal self-samples clinician-taken scrapes. Experimental Design: A total 587 samples were included from...
Abstract Purpose: Offering self-sampling of cervico-vaginal material for high-risk human papillomavirus (hrHPV) testing is an effective method to increase the coverage in cervical screening programs. Molecular triage directly on hrHPV-positive self-samples colposcopy referral opens way full molecular screening. Here, we set out identify a DNA methylation classifier detection precancer (CIN3) and cancer, applicable lavage brush self-samples. Experimental Design: We determined genome-wide...
There are limited data regarding the optimal management of pre-menopausal women with cervical lesions measuring 2-4 cm who desire to preserve fertility.To evaluate feasibility preserving fertility.Neo-adjuvant chemotherapy will be effective in reducing size tumor and enable fertility-sparing surgery without compromising oncologic outcome.Pre-menopausal diagnosed stage International Federation Gynecology Obstetrics (FIGO) IB2, cancer wish fertility receive three cycles platinum/paclitaxel...
Widespread adoption of primary human papillomavirus (HPV)‐based screening has encouraged the search for a triage test which retains high sensitivity detection cervical cancer and precancer, but increases specificity to avoid overtreatment. Methylation analysis FAM19A4 miR124‐2 genes shown promise high‐risk (hr) HPV‐positive women. In our study, we assessed consistency / methylation in series 519 invasive carcinomas ( n = 314 scrapes, 205 tissue specimens) from over 25 countries, using...
Abstract Endometrial cancer incidence is rising and current diagnostics often require invasive biopsy procedures. DNA methylation marker analysis of minimally‐ non‐invasive sample types could provide an easy‐to‐apply patient‐friendly alternative to determine risk. Here, we compared the performance markers detect endometrial in urine, cervicovaginal self‐samples clinician‐taken cervical scrapes. Paired samples were collected from 103 patients diagnosed with stage I IV cancer. Urine at home....
Abstract Objectives Imaging is increasingly used to assess lymph node involvement in clinically early-stage cervical cancer. This retrospective study aimed evaluate the diagnostic accuracy of MRI, CT, and [ 18 F]FDG-PET-CT. Methods Women with International Federation Gynaecology Obstetrics (FIGO) 2009 stage IA2-IIA cancer pretreatment imaging between 2017 were selected from Netherlands Cancer Registry. Patient-based region-based (i.e. pelvic common iliac) nodal status was extracted radiology...
Urine samples provide a potential alternative to physician-taken or self-collected cervical for screening. Screening by primary hrHPV testing requires additional risk assessment (so-called triage) of hrHPV-positive women. Molecular markers, such as DNA methylation, have proven most valuable triage when applied specimens. This study was set out compare and methylation results in paired urine scrapes, evaluate the feasibility analysis detect cancer. (n = 41; native sediment) scrapes 38) from...
Abstract Background The incidence of endometrial cancer is rising, and current diagnostics often require invasive biopsy procedures. Urine may offer an alternative sample type, which easily accessible allows repetitive self-sampling at home. Here, we set out to investigate the feasibility detection in urine using DNA methylation analysis. Results samples patients ( n = 42) healthy controls 46) were separated into three fractions (full void urine, sediment, supernatant) tested for markers...
High-grade cervical intraepithelial neoplasia (CIN2 and CIN3) represents a heterogeneous disease with varying cancer progression risks. Biomarkers indicative for productive human papillomavirus (HPV) infection (HPV E4) transforming HPV (p16ink4a , Ki-67 host-cell DNA methylation) could provide guidance clinical management in women high-grade CIN. This study evaluates the cumulative score of immunohistochemical expression p16ink4a (Scores 0-3) 0-3), referred to as "immunoscore" (IS), 262 CIN2...
Offering self-sampling for HPV testing improves the effectiveness of current cervical screening programs by increasing population coverage. Molecular markers directly applicable on self-samples are needed to stratify HPV-positive women at risk cancer (so-called triage) and avoid over-referral overtreatment. Deregulated microRNAs (miRNAs) have been implicated in development cancer, represent potential triage markers. However, it is unknown whether deregulated miRNA expression reflected...
Treatment strategies for bulky lymph nodes in patients with locally advanced cervical cancer scheduled definitive chemoradiation include nodal boosting radiotherapy, surgical debulking, or both. The aim of this retrospective cohort study was to compare survival and toxicity receiving these treatments them a group that received neither form treatment.
To provide evidence for a reduced surveillance protocol to detect gestational trophoblastic neoplasia after normalisation of human chorionic gonadotrophin (hCG) levels following uterine evacuation complete hydatidiform mole. National retrospective population study. Two UK Trophoblastic Disease Treatment Centres (Sheffield and London), 1 January 1980 30 November 2020. 17 424 patients with hCG their mole were included. Complete moles verified by centralised pathological review. Patients...
Gestational trophoblastic diseases (GTDs) are a group of pregnancy-related premalignant and malignant with generally favorable prognosis when treated adequately. Many different treatment protocols exist worldwide. To our knowledge, this is the first set global consensus-based guidelines for GTD. Four international organizations (European Organisation Treatment Trophoblastic Diseases, European Society Gynecologic Oncology, Cancer Intergroup, International Study Diseases) delegated 53 expert...
Urine sampling is an interesting solution for CIN3 and cervical cancer detection. can be separated in different fractions: full void urine, urine sediment supernatant. We aimed to determine which fraction most competent detection by methylation analysis. samples (27 controls, 30 17 cancer) were processed into 3 fractions tested 5 markers (ASCL1, GHSR, LHX8, SST, ZIC1). determined Spearman correlation coefficients between fractions, compared levels calculated AUCs In general strong...