A5078318929- DNA Repair Mechanisms
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Genetic Mapping and Diversity in Plants and Animals
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Genomics and Chromatin Dynamics
- X-ray Diffraction in Crystallography
- Biochemical and Molecular Research
- Magnetic and transport properties of perovskites and related materials
- Crystallization and Solubility Studies
- Genomics and Phylogenetic Studies
- Cancer therapeutics and mechanisms
- Advanced Nanomaterials in Catalysis
- RNA modifications and cancer
- Thermal Expansion and Ionic Conductivity
- Trace Elements in Health
- Carbon Nanotubes in Composites
- RNA and protein synthesis mechanisms
- SARS-CoV-2 and COVID-19 Research
- Supercapacitor Materials and Fabrication
- Shape Memory Alloy Transformations
- Redox biology and oxidative stress
- Fiber-reinforced polymer composites
- Nerve injury and regeneration
Institute of High Energy Physics
2015-2025
Chinese Academy of Sciences
2015-2025
Nanjing University of Science and Technology
2020-2025
Huzhou Vocational and Technical College
2025
Southwest Jiaotong University
2024
Shanghai Jiao Tong University
2022-2023
Suzhou Kowloon Hospital
2022
Zhujiang Hospital
2020
Sichuan University of Science and Engineering
2014-2020
Southwest University of Science and Technology
2020
Abstract Chitin, the most abundant aminopolysaccharide in nature, is an extracellular polymer consisting of N -acetylglucosamine (GlcNAc) units 1 . The key reactions chitin biosynthesis are catalysed by synthase 2–4 , a membrane-integrated glycosyltransferase that transfers GlcNAc from UDP-GlcNAc to growing chain. However, precise mechanism this process has yet be elucidated. Here we report five cryo-electron microscopy structures devastating soybean root rot pathogenic oomycete Phytophthora...
Cellular DNA lesions are efficiently countered by repair in conjunction with delays cell cycle progression. Previous studies have demonstrated that Rad9, Hus1, and Rad1 can form a heterotrimeric complex (the 9-1-1 complex) plays dual roles checkpoint activation eukaryotic cells. Although the has been proposed to toroidal structure similar proliferating nuclear antigen (PCNA), which essential replication repair, structural basis it performs different functions not elucidated. Here we report...
Abstract HNH endonucleases in bacteriophages play a variety of roles the phage lifecycle as key components DNA packaging machines. The deep-sea thermophilic bacteriophage Geobacillus virus E2 (GVE2) encodes an endonuclease (GVE2 HNHE). Here, crystal structure GVE2 HNHE is reported. This first structural study thermostable from bacteriophage. Structural comparison reveals that possesses typical ββα-metal fold and Zn-finger motif similar to those other bacteriophages, apart containing extra...
SAMHD1 is the only known eukaryotic deoxynucleoside triphosphate triphosphohydrolase (dNTPase) and a major regulator of intracellular dNTP pools. It has been reported to be potent inhibitor retroviruses such as HIV-1 endogenous retrotransposons. Previous crystal structures have revealed that activated by dGTP-dependent tetramer formation. However, recent data indicated primary activator GTP, not dGTP. Therefore, how its dNTPase activity regulated needs further clarified. Here, five catalytic...
Cren7, a newly found chromatin protein, is highly conserved in the Crenarchaeota. The protein shows higher affinity for double-stranded DNA than single-stranded DNA, constrains negative supercoils vitro and associated with genomic vivo. Here we report crystal structures of Cren7 from Sulfolobus solfataricus complex two sequences. binds minor groove causes single-step sharp kink (∼53°) through intercalation hydrophobic side chain Leu28. Loop β3-β4 undergoes significant conformational change...
Abstract HhH-GPD (helix–hairpin–helix-glycine/proline/aspartate) family proteins are involved in DNA damage repair. Currently, mechanism of alkylated repair Crenarchaea has not been fully clarified. The hyperthermophilic model crenarchaeon Saccharolobus islandicus REY15A possesses a novel protein (Sis-HhH-GPD), where its Ser152 corresponds to conserved catalytic Asp other homologs. Herein, we report that Sis-HhH-GPD is bi-functional glycosylase, capable removing both 1-methyladenine (1-meA)...
Abstract The multicopper oxidase CueO is involved in copper homeostasis and (Cu) tolerance Escherichia coli . laccase activity of G304K mutant higher than wild-type CueO. To explain this increase activity, we solved the crystal structure at 1.49 Å. Compared with CueO, showed dramatic conformational changes methionine-rich helix relative regulatory loop (R-loop). We further Cu-soaked enzyme, found that addition Cu ions induced R-loop as a result new Cu-binding sites on enzyme’s surface....
The achievement of significant negative thermal expansion (NTE) over a wide temperature range (ΔTNTE) has posed formidable challenge for NTE materials. In the present study, textured MnCo(Ge,Si)/epoxy composites were prepared by magnetic field-assisted dynamic self-assembly multi-component Mn0.945Co1.055Ge1-xSix particles. utilization particles with tunable transition temperatures significantly amplifies which occurs, surpassing limitations conventional single-component composites....
Cyanobacterial cytochrome c6 (Cyt c6) is crucial for electron transfer between the b6f complex and photosystem I (PSI), playing a key role in photosynthesis enhancing adaptation to extreme environments. This study investigates high-resolution crystal structures of Cyt from Synechococcus elongatus PCC 7942 Synechocystis 6803, focusing on its dimerization mechanisms functional implications photosynthesis. was expressed Escherichia coli using dual-plasmid co-expression system characterized both...
Nerve growth factor (NGF) is a dimeric molecule that modulates the survival, proliferation, and differentiation of nervous cells also known to act on immune system endocrine system. NGFs extracted from mouse submaxillary gland cobra venom have different immunological behaviors, yet underlying mechanism remains unclear. Here we report crystal structure NGF purified Chinese Naja naja atra (cNGF), which unexpectedly reveals 2-tailed lipid embedded between two protomers homodimer. In addition,...
We performed neutron-diffraction experiments and density functional theory calculations to study the magnetostructural coupling in $\mathrm{MnCoGe}{\mathrm{B}}_{x}$ ($x=0$, 0.01, 0.05) alloys. By varying amount of boron addition, we are able freely switch on off MnCoGe It is found that addition stabilizes high-temperature hexagonal phase due reduced interatomic distances enhanced covalent bonding. The hexagonal-orthorhombic structural transition shifts low temperatures with coincides...
High-entropy alloys have attracted tremendous research interest in recent years because of their special functional properties. However, the investigations on high-entropy with thermal- and magnetic-field-induced magnetostructural transformation are still lacking. In this work, we provide a basic strategy to design six-component MnFeCoNiGeSi system, exhibiting low-hysteresis between ferromagnetic orthorhombic paramagnetic hexagonal phases. An increase configurational entropy is helpful make...