A5039563505- Traditional Chinese Medicine Analysis
- Aquaculture disease management and microbiota
- Metabolomics and Mass Spectrometry Studies
- Calpain Protease Function and Regulation
- Plant-based Medicinal Research
- Vibrio bacteria research studies
- Bioactive Natural Diterpenoids Research
- Mosquito-borne diseases and control
- Antimicrobial Peptides and Activities
- Petroleum Processing and Analysis
- Bacteriophages and microbial interactions
- Medicinal plant effects and applications
- Fungal and yeast genetics research
- Computational Drug Discovery Methods
- Flavonoids in Medical Research
- Reproductive tract infections research
- Odor and Emission Control Technologies
- Mechanical and Optical Resonators
- Eicosanoids and Hypertension Pharmacology
- Neurological Disease Mechanisms and Treatments
- Photonic and Optical Devices
- Bacterial biofilms and quorum sensing
- Syphilis Diagnosis and Treatment
- Plant Reproductive Biology
- Synthesis and Biological Activity
Nanyang Medical College
2017-2025
Shandong Agricultural University
2020
Fujian Agriculture and Forestry University
2017-2019
Nanjing University of Chinese Medicine
2012-2013
Protein modification by lysine succinylation is a newly identified post-translational (PTM) of residues and plays an important role in diverse physiological functions, although their associated biological characteristics are still largely unknown. Here, we investigated the effects on regulation within well-known fish pathogen, Aeromonas hydrophila. A high affinity purification method was used to enrich peptides with A. hydrophila ATCC 7966, total 2,174 sites were 666 proteins using LC-MS/MS....
Lysine acetylation and succinylation are both prevalent protein post-translational modifications (PTMs) in bacteria species, whereas the effect of cross-talk between PTMs on bacterial biological function remains largely unknown. Our previously study found lysine succinylated sites proteins play important role metabolic pathways fish pathogenic Aeromonas hydrophila. A total 3189 lysine-acetylation were further identified 1013 this pathogen using LC-MS/MS study. Functional examination these...
Eupatilin, a natural bioactive flavone, is the active ingredient in traditional Chinese medicine Artemisia argyi Levl. et Vant. To enhance antitumor effect of eupatilin, we designed series novel eupatilin–Mannich derivatives and investigated activity against several human cancer cell lines, including gastric cells (AGS), esophageal (Eca-109), breast (MDA-MB-231). Among all derivatives, majority demonstrated superior compared to with compound 3d exhibiting most effective AGS cells....
Outer membrane proteins (OMPs) play essential roles in antibiotic resistance, particularly Gram-negative bacteria; however, they still have many unidentified functions regarding their behavior response to stress. In the current work, quantitative tandem mass tag labeling-based spectrometry was used compare outer related between an oxytetracycline-resistant (OXY-R) and its original control stain (OXY-O) Aeromonas hydrophila. Consequently, a total of 261 commonly altered two biological repeats...
In Gram-negative bacteria, the outer membrane proteins (OMPs) perform a crucial role in antibiotic resistance, but it is largely unknown how they behave response to stress. this study, we treated Aeromonas hydrophila with two different doses of oxytetracycline (OXY) induce Proteins were isolated from sarcosine-insoluble fractions and quantitatively examined by using tandem mass tag labeling-based spectrometry identify differentially expressed proteins. As result, identified 125 differential...
In order to characterize and identify the chemical components in different parts of Artemisia argyi(roots, stems, leaves, seeds), compounds with antioxidant activity were screened. this study, ultra-performance liquid chromatography-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt-quadrupole time-of-flight-tandem mass spectrometry(UPLC-ABTS-Q-TOF-MS) was used as an online combination technique. Poroshell 120 SB-Aq(3.0 mm×150 mm, 2.7 μm) column, acetonitrile(A)-0.2%...
Abstract Xiexin Tang (XXT) is a traditional Chinese medicine (TCM) that has been used in herbal clinics for more than 1800 years. Many studies have shown XXT therapeutic effects on patients with arteriosclerosis owing to its antioxidant activity. However, there little information about the relationship between chemical composition of and In this study, HPLC‐ABTS‐DAD‐Q‐TOF/MS method, which can simultaneously identify individual components rapidly screen compounds, was XXT. The 15 compounds...
Abstract Bidens bipinnata L. is a folk medicinal plant in China that shows significant antihyperlipidemia effectiveness. However, studies of the underlying mechanism study are lacking. In order to explore potential action sites and treating hyperlipidemic, this work undertook tissue distribution molecular docking research on markers B. L., which were obtained through serum pharmacochemistry network database retrieval. The results showed seven compounds (gallic acid, protocatechuic rutin,...
To prepare processed products with different methods, in order to study the impact of auxiliary materials and temperature on chemical components Euphorbia ebracteolata, establish specific chromatograms products. Wel-chorm-C18 column (4.6 mm x 250 mm, 5 microm) was used eluted a gradient program, acetonitrile (A)-water(B). The 25 degrees C, detection wave length set at 226 nm. aim determine content effective products--ebracteolata cpd B, ebracteolata C jolkinolide B respective characteristic...
To study the variation regularity of chemical constituents contained in Euphorbia ebracteolata after vinegar processing.The colorimetric method was adopted for determining total lactone content toxic E. decoction, with Kedde as coloring reagent. The HPLC used detecting jolkinolide B and C, both were active before roasting vinegar, which Kromasil-ODS column (4.6 mm x 250 mm, 5 microm) adopted, detection wavelength 290 nm, temperature at 25 degrees C , gradient elution acetonitrile water flow...
目的采用超高效液相色谱-电喷雾离子源-四极杆-飞行时间质谱技术（UPLC-ESI-Q-TOF/MSE）快速分离、分析颠茄草化学成分。 方法采用Agilent Poroshell 120 EC-C18色谱柱（3 mm×150 mm，2.7 μm），以乙腈为流动相A，醋酸铵缓冲溶液为流动相B，梯度洗脱，流速0.5 mL · min-1，柱温30℃，进样量2 μL；采用电喷雾离子源（ESI），正离子模式下采集数据，通过建立的Agilent PCDL颠茄属化合物数据库初步检索鉴定化合物，并人工复核相对分子质量、特征离子、中性丢失、色谱保留时间等信息，同时利用Agilent MSC结构解析软件，结合对照品信息和数据库信息确证结构，总结裂解规律。 结果初步鉴定出颠茄草中化学成分19个，并归纳了莨菪碱类化合物的裂解规律。 结论采用UPLC-ESI-Q-TOF/MS法快速分析颠茄草化学成分，为进一步阐明颠茄草药效物质基础和质控指标的选择提供依据。
Hyperlipidemia has been highlighted as one of the most prominent and global chronic conditions nowadays. Bidens bipinnata L. (BBL), a folk medicine in contemporary China, efficacy treatment hyperlipidemia (HLP) China. Although some physiological pathological function parameters have investigated, little information about changes small metabolites biofluids reported. In present study, metabolic profiling with high-performance liquid chromatography-linear ion trap/Orbitrap high-resolution mass...