A5091766506- Genetic and phenotypic traits in livestock
- Agricultural and Food Production Studies
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Nuclear Structure and Function
- Parasitic Infections and Diagnostics
- Genetics and Plant Breeding
- RNA modifications and cancer
- Amoebic Infections and Treatments
- Toxoplasma gondii Research Studies
- Microtubule and mitosis dynamics
- ECG Monitoring and Analysis
- Cytomegalovirus and herpesvirus research
- Genetics, Bioinformatics, and Biomedical Research
- DNA Repair Mechanisms
- Autophagy in Disease and Therapy
- Hedgehog Signaling Pathway Studies
- Cellular Mechanics and Interactions
- Genetic Mapping and Diversity in Plants and Animals
- Genetic Syndromes and Imprinting
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Effects of Environmental Stressors on Livestock
- Heart Rate Variability and Autonomic Control
- Non-Invasive Vital Sign Monitoring
IFOM
2018-2023
Universidad Manuela Beltrán
2021
Université Paris Cité
2012-2019
Sorbonne Paris Cité
2012-2019
Centre National de la Recherche Scientifique
2013-2019
Génomes, biologie cellulaire et thérapeutiques
2019
Délégation Paris 7
2012-2019
Inserm
2016-2019
Institut Jacques Monod
2012-2014
Universidad Nacional de Colombia
2003-2013
Autophagy, the process by which proteins or organelles are engulfed autophagosomes and delivered for vacuolar/lysosomal degradation, is induced to ensure survival under starvation other stresses. A selective autophagic pathway 60S ribosomal subunits elicited nitrogen in yeast—ribophagy—was recently described requires Ubp3-Bre5 deubiquitylating enzyme. This discovery implied that an E3 ligases act upstream, whether inhibiting providing initial required signal. In this paper, we show...
Clathrin light chains (CLCa and CLCb) are major constituents of clathrin-coated vesicles. Unique functions for these evolutionary conserved paralogs remain elusive, their role in clathrin-mediated endocytosis mammalian cells is debated. Here, we find structurally characterize a direct selective interaction between CLCa the long isoform actin motor protein myosin VI, which expressed exclusively highly polarized tissues. Using genetically-reconstituted Caco-2 cysts as proxy epithelia, provide...
The nuclear pore complex (NPC) serves as both the unique gate between nucleus and cytoplasm a major platform that coordinates nucleocytoplasmic exchanges, gene expression, genome integrity. To understand how NPC integrates these functional constraints, we dissected here posttranslational modifications of basket protein Nup60 analyzed they intervene to control plasticity NPC. Combined approaches highlight role monoubiquitylation in regulating association dynamics its partner, Nup2, with...
Abstract Ubiquitination is a highly dynamic and versatile posttranslational modification that regulates protein function, stability, interactions. To investigate the roles of ubiquitination in primitive eukaryotic lineage, we utilized early‐branching eukaryote G iardia intestinalis . Using combination biochemical, immunofluorescence‐based, proteomics approaches, assessed status during process differentiation We observed different types ubiquitin modifications present specific cellular...
The nuclear pore complex (NPC) is the major conduit for nucleocytoplasmic transport and serves as a platform gene regulation DNA repair. Several nucleoporins undergo ubiquitylation SUMOylation, these modifications play an important role in dynamics plasticity. Here, we perform detailed analysis of post-translational yeast basket proteins under normal growth conditions well upon cellular stresses, with focus on SUMOylation. We find that balance between SUMOylation deSUMOylation Nup60 Nup2 at...
Deregulated epidermal growth factor receptor (EGFR) signaling is a key feature in different stages of oncogenesis. One important mechanism whereby cancer cells achieve increased and uncontrolled EGFR escaping down-modulation the receptor. Ubiquitylation plays decisive role this process, as it regulates internalization, trafficking degradation. Deubiquitinating enzymes (DUBs) may oppose ubiquitylation antagonizing or even promoting Here, we use qualitative quantitative assays to measure...
The actin motor protein myosin VI is a multivalent with diverse functions.Here, we identified and characterised ubiquitous interactor, the oral-facial-digital syndrome 1 (OFD1) protein, whose mutations cause malformations of face, oral cavity, digits polycystic kidney disease.We found that regulates localisation OFD1 at centrioles and, as consequence, recruitment distal appendage Cep164.Myosin depletion in non-tumoural cell lines causes an aberrant along centriolar walls, which due to...
Covalent attachment of ubiquitin to target proteins, or ubiquitylation, has emerged as one the most prevalent posttranslational modifications (PTMs), regulating nearly every cellular pathway. The diversity functions associated with this particular PTM stems from myriad ways in which a protein can be modified by ubiquitin, e.g., monoubiquitin, multi-monoubiquitin and polyubiquitin linkages. In current study, we took systematic approach analyze ubiquitylation profiles yeast Saccharomyces...
Summary Mixed model methodology has been the main statistical tool for unbiased genetic animal evaluation and selection of domestic animals, such as bovines, over thirty years. Since use linear mixed models to obtain best prediction breeding value individuals in a population was proposed, there have scientific advances at computational level that led implement more complex describing different biological situations data structures. This work briefly reviews history cattle emphasizing on...
Summary Objective: the objective of this research was to estimate genetic parameters and predict breeding values for live weight in a Colombian Bos taurus-Bos indicus multibreed beef cattle population using random regression models (RRM). Methods: included 352 offspring from 37 sires nine breeds mated Gray Brahman females. The sire were Brahman, Red Guzerat, Blanco Orejinegro, Romosinuano, Simmental, Braunvieh, Normand Limousin. A longitudinal structured data set comprising 1,090 records...
Resumen Objetivo: dada la necesidad de evaluar varias razas bovinas en Colombia para caracteres crecimiento y composición corporal, el objetivo este estudio fue obtener mejores estimadores lineales insesgados efectos heterosis grupo racial tales caracteres. Métodos: las características evaluadas fueron peso a diferentes edades: nacimiento (PN), aproximadamente los 4 meses (P4), destete (PD), año (PA), 15 (P15). Las caracteristicas corporal área ojo del lomo (AOL) grasa dorsal (GD) medidas...
Giardia intestinalis is a unicellular parasite of worldwide distribution. It causes an intestinal illness known as giardiasis, and it probably the earliest diverging eukaryotic microorganism. Previously, changes have been reported in expression mRNAs at several stages life cycle; however specific enzymatic activity not explored.The pyruvate ferredoxin oxidoreductase (PFOR) alcohol dehydrogenase E (ADHE) enzymes was measured cyst trophozoite stages, during excystation process.Recombinant...