A5102710291- Drug Transport and Resistance Mechanisms
- Pharmacological Effects and Toxicity Studies
- Amino Acid Enzymes and Metabolism
- Antibiotics Pharmacokinetics and Efficacy
- Pharmacogenetics and Drug Metabolism
- Lung Cancer Treatments and Mutations
- Neutropenia and Cancer Infections
- Adenosine and Purinergic Signaling
- Pancreatic and Hepatic Oncology Research
- Metabolism and Genetic Disorders
- Pharmacy and Medical Practices
- Gastroesophageal reflux and treatments
- Cancer Treatment and Pharmacology
- Neonatal Health and Biochemistry
- Pharmaceutical Practices and Patient Outcomes
- Pharmaceutical studies and practices
- Cancer therapeutics and mechanisms
- Chemotherapy-induced organ toxicity mitigation
- Colorectal Cancer Treatments and Studies
- Inhalation and Respiratory Drug Delivery
- Renal Transplantation Outcomes and Treatments
- Liver Disease Diagnosis and Treatment
- Renal cell carcinoma treatment
- Glycosylation and Glycoproteins Research
- Pneumocystis jirovecii pneumonia detection and treatment
Kyoto University Hospital
2010-2025
Wakayama Medical University
2025
Kyoto University
2003-2024
Shiga University of Medical Science Hospital
2015-2024
Shiga University of Medical Science
2010-2024
Nagoya University Hospital
2023
Therapeutics Clinical Research
2022
TDK (Japan)
2021-2022
Intel (United States)
2022
Ritsumeikan University
2021
A cDNA coding a new H+/organic cation antiporter, human kidney-specific multidrug and toxin extrusion 2 (hMATE2-K), has been isolated from the kidney. The hMATE2-K had an open reading frame that encodes 566–amino acid protein, which shows 94, 82, 52, 52% identity with hMATE2, hMATE2-B, hMATE1, rat MATE1, respectively. Reverse transcriptase–PCR revealed mRNA but not hMATE2 was expressed predominantly in kidney, hMATE2-B ubiquitously found all tissues examined except immunohistochemical...
In human proximal tubules, organic cations are taken up from blood into cells by cation transporter 2 [hOCT2/solute carrier (SLC) 22A2] and then eliminated the lumen apical H<sup>+</sup>/organic antiporters, multidrug toxin extrusion 1 (hMATE1/SLC47A1) hMATE2-K (SLC47A2). To evaluate drug interactions of cationic drugs in secretion process, epithelial engineered to express both hOCT2 hMATE transporters required simultaneously with renal basolateral transporters. present study, therefore, we...
Multidrug and toxin extrusion 1 (MATE1/SLC47A1) is important for excretion of organic cations in the kidney liver, where it located on luminal side. Although its functional regulatory characteristics have been clarified, pharmacokinetic roles vivo yet to be elucidated. In present study, clarify relevance MATE1 vivo, targeted disruption murine <i>Mate1</i> gene was carried out. The lack Mate1 expression liver confirmed by reverse transcription-polymerase chain reaction Western blot analysis....
Perfluorooctanoic acid, an environmental contaminant, is found in both wild animals and human beings. There are large species sex differences the renal excretion of perfluorooctanoic acid. In present study, we aimed to characterize organic anion transporters 1-3 (OAT1-3) beings rats investigate whether elimination kinetics acid from kidneys can be attributed affinities these for We used (h) rat (r) OAT transient expression cell systems measured [(14)C] transport activities. Both OAT1 OAT3...
During anticoagulant therapy, major bleeding is one of the most severe adverse effects. This study aimed to evaluate relationships between ABCB1, ABCG2, and CYP3A5 polymorphisms plasma trough concentrations apixaban, a direct inhibitor coagulation factor X.A total 70 apixaban from 44 Japanese patients with atrial fibrillation were analyzed. In these analyses, concentration/dose (C/D) ratio was used as pharmacokinetic index all data stratified according presence ABCB1 (ABCB1 1236C>T,...
In mammals, most physiological, biochemical, and behavioral processes show a circadian rhythm. the present study, we examined diurnal rhythm of H+-peptide cotransporter (PEPT1), which transports small peptides peptide-like drugs in intestine kidney, using rats maintained 12-h photoperiod with free access to chow. The transport [14C]glycylsarcosine (Gly-Sar), typical substrate for PEPT1 by situ intestinal loop everted intestine, was greater dark phase than light phase. protein mRNA levels...
[reaction: see text] Described is a novel synthetic route for dipeptide isosteres containing (Z)-alkene and (E)-fluoroalkene units as cis-amide bond equivalents via organocopper-mediated reduction of gamma-acetoxy- or gamma,gamma-difluoro-alpha,beta-unsaturated-delta-lactams. The synthesized were evaluated in terms their affinities the peptide transporter PEPT1. trans-Amide tended to possess higher PEPT1 compared corresponding equivalents.
PEPT1 and PEPT2 are H + -coupled peptide transporters expressed preferentially in the intestine kidney, respectively, which mediate uphill transport of oligopeptides peptide-like drugs such as β-lactam antibiotics. In present study, we have compared recognition antibiotics by LLC-PK 1 cells stably transfected with or cDNA. Cyclacillin (aminopenicillin) ceftibuten (anionic cephalosporin without an α-amino group) showed potent inhibitory effects on glycylsarcosine uptake PEPT1-expressing...
Abstract TLRs serve important immune and nonimmune functions in human intestinal epithelial cells (IECs). Proinflammatory Th1 cytokines have been shown to promote TLR expression function IECs, but the effect of key Th2 (IL-4, IL-5, IL-13) on signaling IECs has not elucidated so far. We stimulated model with examined mRNA protein by Northern blotting, RT-PCR, real-time Western flow cytometry. was determined I-κBα phosphorylation assays, ELISA for IL-8 secretion after stimulation ligands...
The effect of oxaliplatin against colorectal cancer is superior to that cisplatin, but the molecular mechanism(s) involved not clear. We found previously oxaliplatin, was transported by human (h) and rat organic cation transporter 3 (OCT3)/<i>SLC22A3</i>. In present study, we examined whether hOCT3 significantly in oxaliplatin-induced cytotoxicity accumulation platinum cancer. level mRNA colon 9.7-fold higher cancerous than normal tissues six Japanese patients (<i>P</i> = 0.0247)....
H+/organic cation antiporters (multidrug and toxin extrusion: MATE1 MATE2-K) play important roles in the renal tubular secretion of cationic drugs. We have recently identified a regulatory single nucleotide polymorphism (SNP) gene (−32G>A). There is no other information about SNPs MATE gene. In this study, we evaluated functional significance genetic polymorphisms MATE2-K. sequenced all exons MATE2-K genes 89 Japanese subjects coding (cSNPs) encoding (V10L, G64D, A310V, D328A N474S) (K64N...
Human organic cation transporter 2 (OCT2/SLC22A2), which is specifically expressed in the kidney, plays critical roles renal secretion of cationic compounds. Tissue expression and membrane localization OCT2 are closely related to tissue distribution, pharmacological effects, and/or adverse effects its substrate drugs. However, molecular mechanisms underlying kidney-specific have not been elucidated. In present study, therefore, we examined contribution DNA methylation promoter region for...