A5010865710- Lipid Membrane Structure and Behavior
- Drug Transport and Resistance Mechanisms
- Cholesterol and Lipid Metabolism
- Sphingolipid Metabolism and Signaling
- Lipid metabolism and biosynthesis
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Renal cell carcinoma treatment
- Cancer Treatment and Pharmacology
- Lung Cancer Treatments and Mutations
- Lipid metabolism and disorders
- Lipoproteins and Cardiovascular Health
- Renal and related cancers
- Mitochondrial Function and Pathology
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer, Lipids, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Research Studies
- Metabolism and Genetic Disorders
- Radiopharmaceutical Chemistry and Applications
- Inhalation and Respiratory Drug Delivery
- Neuroscience and Neuropharmacology Research
- Pharmacy and Medical Practices
- Pediatric Hepatobiliary Diseases and Treatments
Shiga University of Medical Science Hospital
2016-2025
Shiga University of Medical Science
2011-2025
Hokkaido University
2018-2024
Keio University Hospital
2024
Kyoto University Hospital
2021
Kyoto Pharmaceutical University
2021
National Institute of Technology, Yonago College
2021
Ritsumeikan University
2021
Kyoto University
2003-2020
Yokohama City University
2010-2013
Nuclear lipid droplets (LDs) in hepatocytes are derived from precursors of very-low-density lipoprotein the ER lumen, but it is not known how cells lacking secretory function form nuclear LDs. Here, we show that inner membrane (INM) U2OS harbors triglyceride synthesis enzymes, including ACSL3, AGPAT2, GPAT3/GPAT4, and DGAT1/DGAT2, generates LDs situ. mTOR inhibition increases by inducing translocation lipin-1 phosphatidic acid (PA) phosphatase. Seipin, a protein essential for normal...
Background Diacylglycerol (DG) kinase (DGK) phosphorylates DG to produce phosphatidic acid (PA). Of the 10 subtypes of mammalian DGKs, DGKβ is a membrane-localized subtype and abundantly expressed in cerebral cortex, hippocampus, caudate-putamen. However, its physiological roles neurons higher brain function have not been elucidated. Methodology/Principal Findings We, therefore, developed KO mice using Sleeping Beauty transposon system, found that long-term potentiation hippocampal CA1...
Abstract Human ABCB4 (multidrug resistance [MDR]3 P-glycoprotein) is expressed in the canalicular membrane of hepatocyte. has been shown to be required for phosphatidylcholine (PC) secretion into bile and translocate PC across plasma membrane. To further investigate function ABCB4, we established a cell line stably expressing (human embryonic kidney [HEK]/ABCB4). The efflux phospholipids from HEK/ABCB4 cells was remarkably increased by addition taurocholate. In addition, cholesterol also...
We examined the intradermal delivery of a hydrophilic polyphenol chlorogenic acid by in vitro study using excised guinea pig dorsal skin and Yucatan micropig skin. Skin accumulation as well solubility aqueous vehicles was much greater than for other polyphenols such quercetin genistein. However, since enhancement seemed to be necessary exhibit its protective effects against oxidative damage skin, we microemulsions vehicles. Using consisting 150 mM NaCl solution, isopropyl myristate,...
Abstract Phosphatidylglycerol (PG) and cardiolipin (CL) are synthesized in mitochondria regulate numerous biological functions. In this study, a novel fluorometric method was developed for measuring PG CL using combinations of specific enzymes Amplex Red. This assay quantified the sum (PG + CL) regardless species fatty acyl chain. The calibration curve measurement linear detection limit 1 μM (10 pmol reaction mixture). new applied to determinations content HEK293 cells purified mitochondria,...
The hydrogen production from bean curd manufacturing waste by anaerobic microflora was investigated using batch experiments at 35 °C. obtained fermented soybean-meals and maintained a sucrose-limited medium in continuous culture. A solution of an organic substrate without solid component such as rough fiber used for the experiments. After inoculation, immediately occurred almost ceased 12 h. final concentration gas produced 63% H2. During production, carbohydrate rapidly degraded while...
The OTOF gene (Locus: DFNB9), encoding otoferlin, is reported to be one of the major causes non-syndromic recessive sensorineural hearing loss, and also most common cause auditory neuropathy spectrum disorder (ANSD). In present study, we performed mutation analysis using massively parallel DNA sequencing (MPS). purpose this study was reveal frequency precise genetic clinical background OTOF-related loss in a large population. A total 2,265 Japanese (SNHL) patients compatible with autosomal...
A heterozygous mutation in the Wolfram syndrome type 1 gene (WFS1) causes autosomal dominant nonsyndromic hereditary hearing loss, DFNA6/14/38, or Wolfram-like syndrome. To date, more than 40 different mutations have been reported to be responsible for DFNA6/14/38. In present study, WFS1 variants were screened a large series of Japanese loss (HL) patients clarify prevalence and clinical characteristics DFNA6/14/38 Massively parallel DNA sequencing 68 target genes was performed 2,549...
In this work, we developed a novel enzymatic method for measuring phosphatidic acid (PA) in cultured cells. The reaction sequence of the involves hydrolysis PA to produce glycerol-3-phosphate (G3P), which is then oxidized by G3P oxidase generate hydrogen peroxide. presence peroxidase, peroxide reacted with Amplex Red highly fluorescent resorufin. We found that lipase from Pseudomonas sp. can completely hydrolyze and FAs. calibration curve measurement was linear between 20 250 microM,...
The enzyme catalysing the conversion of PE (phosphatidylethanolamine) into PC (phosphatidylcholine), PEMT (PE N-methyltransferase), exists as two isoforms, PEMT-L (longer isoform PEMT) and PEMT-S (shorter PEMT). In present study, to compare functions isoforms PEMT, we established HEK (human embryonic kidney)-293 cell lines stably expressing PEMT-S. Both were localized in ER (endoplasmic reticulum). PEMT-L, but not PEMT-S, was N-glycosylated with high-mannose oligosaccharides. enzymatic...
The aim of the present study was to determine influence severe renal dysfunction (estimated glomerular filtration rate <30 ml/min/1.73 m<sup>2</sup>, including hemodialysis) on pharmacokinetics and therapeutic effects febuxostat using a population pharmacokinetic analysis. This recruited patients with hyperuricemia who were initially treated allopurinol, but switched febuxostat, it consists 2 sub-studies: (26 patients) retrospective efficacy evaluation (51 patients)....
The patients' inhalation flow pattern is one of the significant determinants for clinical performance therapy. However, development meters various devices has been unable to keep up with increasing number newly launched devices. In present study, we developed simple attachment orifices monitoring system, which are suitable all commercial inhalers, and investigated efficacy system on instruction patients co-prescribed dry powder inhaler (DPI) soft mist (SMI). First, constructed that were...
<i>Purpose of the Study:</i> We aimed to compare short-term outcome patients with acute low-tone sensorineural hearing loss (ALHL) treated steroid alone, diuretics alone or combination treatment. <i>Procedures:</i> Between April 2000 and March 2009, we retrospectively reviewed medical records 156 a diagnosis ALHL. All were followed up until improvement for 8 weeks from initial examination. Patients (n = 49), 40), treatment 46) they received neither nor 21)....
Bile salts have potent detergent properties and damaging effects on cell membranes, leading to liver injury. However, the molecular mechanisms for protection of hepatocytes against bile are not fully understood. In this study, we demonstrated that cytotoxicity nine human major HepG2 cells primary was prevented by phosphatidylcholine (PC). contrast, cholesterol had no direct cytotoxic but suppressed cytoprotective PC. PC reduced cell-association salt, which reversed cholesterol. Light...