A5068283403- RNA modifications and cancer
- Neurogenetic and Muscular Disorders Research
- Vitamin D Research Studies
- RNA Research and Splicing
- Cardiac and Coronary Surgery Techniques
- Congenital Anomalies and Fetal Surgery
- Muscle Physiology and Disorders
- Vitamin C and Antioxidants Research
- Pancreatic function and diabetes
- Hepatitis C virus research
- Urban and spatial planning
- Aortic Disease and Treatment Approaches
- Vitamin K Research Studies
- Liver physiology and pathology
- Gastric Cancer Management and Outcomes
- Pharmacological Effects and Assays
- Liver Disease Diagnosis and Treatment
- Neonatal Health and Biochemistry
- Drug Transport and Resistance Mechanisms
- Diet and metabolism studies
- Gastrointestinal Tumor Research and Treatment
- Hepatitis B Virus Studies
- Mass Spectrometry Techniques and Applications
- Aortic aneurysm repair treatments
- Cardiac, Anesthesia and Surgical Outcomes
Kobe Pharmaceutical University
2015-2025
Kobe Tokiwa University
2024
National Agriculture and Food Research Organization
2006-2019
Institute of Vegetable and Floriculture Science
1994-2019
Kitasato University
2001-2018
Chikamori Hospital
2009-2014
Yokohama University of Pharmacy
2008-2010
University Gastroenterology
2009
Niigata University of Pharmacy and Medical and Life Sciences
2008
Kyoto Prefectural University of Medicine
2008
The recent discovery of nonvisual photoreceptors in various organs has raised expectations for uncovering their roles and underlying mechanisms. In this work, we identified a previously unrecognized hormone-releasing mechanism the pituitary Japanese rice fish (medaka) induced by light. Ca 2+ imaging analysis revealed that melanotrophs, type endocrine cell secretes melanocyte-stimulating hormone, robustly increase concentration intracellular during short-wavelength light exposure. Moreover,...
Anthocyanins were detected in extracts from the peels of 123 accessions eggplant (Solanum melongena) and its related species. Their anthocyanin profiles classified into four types, including known Japanese type (type 1) non-Japanese 2). Although most had one two profiles, accession a novel profile 3). Two species showed another 4). The major anthocyanins identified as delphinidin 3-(p-coumaroylrutinoside)-5-glucoside (nasunin) 1), 3-rutinoside 2), 3-glucoside 3), petunidin (petunidin 3RGc5G)...
Hepatocytes are regarded as the only effective cell source for transplantation to treat liver diseases; however, their availability is limited due a donor shortage. Thus, novel must be developed. We recently reported that mature rodent hepatocytes can reprogrammed into progenitor-like cells with repopulative capacity using small molecule inhibitors. Here, we demonstrate hepatic progenitor obtained from human infant same strategy. These cells, named chemically induced progenitors (hCLiPs),...
Pathogenic variants in WDR45 on chromosome Xp11 cause neurodegenerative disorder beta-propeller protein-associated neurodegeneration (BPAN). Currently, there is no effective therapy for BPAN. Here we report a 17-year-old female patient with BPAN and show that antisense oligonucleotide (ASO) was vitro. The had developmental delay later showed extrapyramidal signs since the age of 15 years. MRI findings iron deposition globus pallidus substantia nigra T2 MRI. Whole genome sequencing RNA...
Protein binding properties of 22-oxa-1 alpha,25-dihydroxyvitamin D3 (22-oxa-1,25-D3), a synthetic analogue 1 (1,25-D3), were compared with those vitamin derivatives. The order affinity to the chick embryonic intestinal receptor was 1,25-D3 greater than 22-oxa-1,25-D3 25-hydroxyvitamin (25-D3) 24R, 25-dihydroxyvitamin (24, 25-D3) (D3), while that rat plasma D-binding protein (DBP) 25-D3 24,25-D3 22-oxa-1,25-D3. potencies and DBP about 1/8 1/600 respective values 1,25-D3. When distribution...
Spinal muscular atrophy (SMA) is a neuromuscular disorder caused by
Three full-length cDNAs (CHS1, CHS2 and CHS3) encoding chalcone synthase (CHS; EC 2.3.1.74) were isolated from young leaves of Camellia sinensis. Each cDNA encoded 389 amino acid residues, which showed 93-96% identity to one another. Oligonucleotides synthesized on the basis 5'-untranslated sequences their corresponding transcripts could be distinguished. The CHS1, CHS3 abundant in stems. After an initial treatment with water darkness, fell very low levels leaves. These increased by a...
Although hepatitis B virus (HBV) DNA can be detected in liver or sera of patients without serum surface antigen (HBsAg), its clinical relevance hepatocarcinogenesis remains controversial. This observational cohort study was conducted to clarify the risk factors, including presence HBV and core antibody (anti-HBc), for hepatocellular carcinoma (HCC) with C (HCV)-related cirrhosis (LC).The comprised 123 LC due HCV, negative HBsAg. The factors HCC development were analyzed by univariate...
Recent studies have suggested that an occult hepatitis B virus (HBV) infection negative for HBsAg but positive HBV-DNA contributes to hepatocellular carcinoma (HCC) development in patients with chronic C. Some follow-up the clinical importance of HBV infections HCC even after interferon (IFN) therapy, a recent study denies significance impact infection. Focusing on whom C (HCV) eradication by therapy had failed, we conducted this order assess these patients.We enrolled 141 (histological...
The enzyme catalysing the conversion of PE (phosphatidylethanolamine) into PC (phosphatidylcholine), PEMT (PE N-methyltransferase), exists as two isoforms, PEMT-L (longer isoform PEMT) and PEMT-S (shorter PEMT). In present study, to compare functions isoforms PEMT, we established HEK (human embryonic kidney)-293 cell lines stably expressing PEMT-S. Both were localized in ER (endoplasmic reticulum). PEMT-L, but not PEMT-S, was N-glycosylated with high-mannose oligosaccharides. enzymatic...
Spinal muscular atrophy (SMA) is a common devastating neuromuscular disorder, usually involving homozygous deletion of the SMN1 gene. Newly developed drugs can improve motor functions infants with SMA when treated in early stage. To ensure diagnosis, newborn screening for (SMA-NBS) via PCR-based genetic testing dried blood spots (DBSs) has been spreading throughout Japan. In Hyogo Prefecture, we performed pilot study SMA-NBS to assess who underwent routine metabolic between February 2021 and...
Treatment approaches are lacking for Fukuyama congenital muscular dystrophy (FCMD), the second most common type of pediatric after Duchenne (DMD) in Japanese population. Recent studies demonstrating involvement prostaglandin (PG) D2 DMD progression has led to development novel inhibitors targeting hematopoietic PGD2 synthase. This study aimed determine role FCMD etiology 42 patients with and 77 healthy age-matched individuals. Concentrations tetranor-PGDM tetranor-PGEM, metabolites PGE2,...
The binding properties, with blood proteins, and tissue distribution of 22-oxa-1α, 25-dihydroxyvitamin D3 (22-oxacalcitriol; OCT), a noncalcemic analogue 1α, 25-dihydroxy-vitamin [1, 25(OH)2D3], in rats were investigated. affinity OCT to plasma vitamin D protein (DBP) is extremely low mainly circulates the as an intact form nonspecifically bound lipoproteins, especially chylomicrons density lipoprotein (LDL). intravenously injected into normal rapidly disappeared from blood, appeared bile...
The combination of NMR, MS, and CD data permitted the structural elucidation including absolute configuration known alkaloids unknown components in extract matrix solution Nandina domestica without isolation sample purification prior to coupling experiments. Unstable natural stereoisomers were identified by LC-NMR LC-MS. Five alkaloids, (S)-isoboldine, (S)-domesticine, (S)-nantenine, sinoacutine, menispermine, from N. domestica. O-Methylpallidine (E, E)-, Z)-, (Z, Z)-terrestribisamide also...
Abstract Most opsins are G protein-coupled receptors that utilize retinal both as a ligand and chromophore. Opsins’ main established mechanism is light-triggered activation through 11- cis -to-all- trans photoisomerization. Here we report vertebrate non-visual opsin functions Gi-coupled receptor deactivated by light can thermally self-regenerate. This opsin, Opn5L1, binds exclusively to all- -retinal. More interestingly, the light-induced deactivation -to- isomerization followed formation of...