A5037977275- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- CAR-T cell therapy research
- Immune Response and Inflammation
- Dermatology and Skin Diseases
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- Liver Diseases and Immunity
- Microscopic Colitis
- Inflammatory Bowel Disease
- Genetic Neurodegenerative Diseases
- Pharmacological Effects of Natural Compounds
- Neuroinflammation and Neurodegeneration Mechanisms
- Asthma and respiratory diseases
- CRISPR and Genetic Engineering
- Respiratory viral infections research
- Mycobacterium research and diagnosis
- Reproductive System and Pregnancy
- Advanced Biosensing Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Graphene and Nanomaterials Applications
- Virus-based gene therapy research
Sejong University
2015-2024
Intel (United States)
2011
Howard Hughes Medical Institute
1997-2006
University of California, San Francisco
2006
Vanderbilt University
1997-2004
New York University
2000
Princeton University
2000
Chiba University
2000
Emory University
2000
Albert Einstein College of Medicine
2000
Experimental autoimmune encephalomyelitis (EAE) serves as a prototypic model for T cell–mediated autoimmunity. Vα14 natural killer (NKT) cells are subset of lymphocytes that recognize glycolipid antigens presented by the nonpolymorphic major histocompatibility complex (MHC) class I–like protein CD1d. Here, we show activation NKT glycosphingolipid α-galactosylceramide (α-GalCer) protects susceptible mice against EAE. β-GalCer, which binds CD1d but is not recognized cells, failed to protect...
Abstract NK T cells recognize glycolipid Ags such as α-galactosylceramide (α-GalCer) presented by the MHC class I-like molecule CD1d. In this paper we have studied in vivo effects of α-GalCer on generation adaptive immune responses. Treatment mice with resulted rapid activation and production cytokines IL-4 IFN-γ. However, after initial stimulation, became polarized for IL-4. Further, soon 6 days injection, a marked increase serum IgE levels was observed. Administration at time priming...
A necessary role for cytotoxic T lymphocytes in protection against Mycobacterium tuberculosis (MTB) has been suggested by studies of the beta2-microglobulin-deficient mouse, which is unable to present antigens through MHC class I and I-like molecules invariably succumbs early after infection. To identify relative contributions distinct putative I-dependent cell populations tuberculosis, we compared a variety gene-disrupted mouse strains susceptibility MTB Among tested, most susceptible mice,...
Natural killer T (NKT) cells are a unique population of lymphocytes that coexpress semiinvariant cell and natural receptors, which particularly abundant in the liver. To investigate possible effect these on development liver stages malaria parasites, glycolipid, α-galactosylceramide (α-GalCer), known to selectively activate Vα14 NKT context CD1d molecules, was administered sporozoite-inoculated mice. The administration α-GalCer resulted rapid, strong antimalaria activity, inhibiting...
The earliest contact between antigen and the innate immune system is thought to direct subsequent antigen-specific T cell response. We hypothesized that cells of system, such as natural killer (NK) cells, NK1.1+ (NKT cells), γ/δ may regulate development allergic airway disease. demonstrate here depletion (NK NKT cells) before immunization inhibits pulmonary eosinophil CD3+ infiltration well increased levels interleukin (IL)-4, IL-5, IL-12 in bronchoalveolar lavage fluid a murine model...
ABSTRACT CD1d-deficient mice have normal numbers of T lymphocytes and natural killer cells but lack Vα14 + cells. Respiratory syncytial virus (RSV) immunopathogenesis was evaluated in 129×C57BL/6, C57BL/6, BALB/c CD1d −/− mice. CD8 were reduced all strains, as shown by cell surface staining major histocompatibility complex class I tetramer analysis, resulted strain-specific alterations illness, viral clearance, gamma interferon (IFN-γ) production. Transient activation NK +/+ α-GalCer illness...
Abstract NKT cells express both NK cell-associated markers and TCR. Classically, these NK1.1+TCRαβ+ have been described as being either CD4+CD8− or CD4−CD8−. Most interact with the nonclassical MHC class I molecule CD1 through a largely invariant Vα14-Jα281 TCR chain in conjunction Vβ2, -7, -8 chain. In present study, we describe presence of significant numbers within lymphokine-activated killer cell cultures from wild-type C57BL/6, CD1d1−/−, Jα281−/− mice that lack classical cells. Unlike...
Abstract IL-12p40 is induced in macrophages and dendritic cells (DC) after activation by microbial TLR ligands cytokines constitutes a component of IL-12 IL-23. In an effort to understand the location kinetics these during course immune response, we generated knockin (gene-targeted) mice that express p40 gene linked via viral internal ribosome entry site element with fluorescent reporters, eYFP or eGFP. Macrophages DC from faithfully reported biallelic induction using marker. s.c....
The R6/2 mouse is the most frequently used model for experimental and preclinical drug trials in Huntington's disease (HD). When was first developed, it carried exon 1 of huntingtin gene with ∼150 cytosine-adenine-guanine (CAG) repeats. presented a rapid aggressive phenotype that shared many features human condition particularly similar to juvenile HD. However, instability CAG repeat length due different breeding practices has led both decreases increases average lengths among colonies....
Abstract Background Infliximab (IFX), a TNF-α blocking chimeric monoclonal antibody, induces clinical response and mucosal healing in patients with inflammatory bowel disease (IBD). However, systemic administration of this agent causes unwanted side effects. Oral delivery antibody therapeutics might be an effective treatment strategy for IBD compared to intravenous administration. Results All three carriers had high encapsulation efficiency, narrow size distribution, minimal exposure. There...
Abstract Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that accompanied by the emergence of autoreactive T cells and reduction in regulatory cells. Humans mice with SLE have reduced numbers CD1d-restricted NK cells, suggesting role for these regulation SLE. In this study, we show CD1d deficiency exacerbates nephritis induced hydrocarbon oil pristane. This exacerbation associated with: 1) TNF-α IL-4 production especially during induction phase; 2) expansion marginal zone...
Conventional T cells, NK cells and NKT have been implicated in the anti-tumor activities induced by IL-12. Here we show that IL-12-induced immune responses are partially impaired cell-deficient RAG-2–/– mice, CD1–/– mice. In response to a small dose (<1000 U) of IL-12, mice demonstrated reduced cytotoxicity, serum IFN-γ elevation anti-metastatic activities; contrast, high (>2000U) were indistinguishable from wild-type The defective low-dose IL-12 corrected adoptive transfer but not cells....
Mechanisms responsible for the development of autoimmune skin disease in humans and animal models with lupus remain poorly understood. In this study, we have investigated role CD1d, an antigen-presenting molecule known to activate natural killer T cells, inflammatory dermatitis lupus-susceptible MRL-lpr/lpr mice. particular, established mice carrying a germ-line deletion CD1d genes. We demonstrate that CD1d-deficient mice, as compared wild-type littermates, more frequent severe disease,...
Abstract Graphene oxide (GO) modulates the functions of antigen-presenting cells including dendritic (DCs). Although carbon nanotubes affect expression MHC class I-like CD1d molecule, whether GO can influence immune responses CD1d-dependent invariant natural killer T (iNKT) remains unclear. Here, we investigated impact on inflammatory mediated by α-galactosylceramide (α-GalCer), an iNKT cell agonist. We found that in vivo treatment substantially inhibited capacity α-GalCer to induce...
Genetically engineered cells via CRISPR/Cas9 system can serve as powerful sources for cancer immunotherapeutic applications. Furthermore, multiple genetic alterations are necessary to overcome tumor-induced immune-suppressive mechanisms. However, one of the major obstacles is technical difficulty with efficient gene manipulation suspension due low transfection efficacy. Herein, we established a carrier-free multiplexed editing platform in simplified method, which enhance function cytotoxic...
The polysaccharide component of Angelica gigas induces immuno-stimulatory effects on innate immune cells. However, it is unclear whether A. gigas' adjuvant activity the system can elicit anti-cancer responses.A water-soluble was prepared. How this ISAg modulated activation cells such as dendritic (DCs) examined. ISAg-induced cytotoxic via natural killer (NK) and NKT also tested using a tumor-bearing mouse model.ISAg treatment induced nitric oxide (NO) production cytokine gene expression...