A5074990799- Immune Cell Function and Interaction
- Chromatin Remodeling and Cancer
- T-cell and B-cell Immunology
- Genomics and Chromatin Dynamics
- Immunotherapy and Immune Responses
- Cancer Mechanisms and Therapy
- Fungal and yeast genetics research
- DNA Repair Mechanisms
- RNA Research and Splicing
- NF-κB Signaling Pathways
- IL-33, ST2, and ILC Pathways
- CAR-T cell therapy research
- Immunodeficiency and Autoimmune Disorders
- MicroRNA in disease regulation
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Pluripotent Stem Cells Research
- Immune cells in cancer
- Cancer therapeutics and mechanisms
- Heat shock proteins research
- Signaling Pathways in Disease
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
Seoul National University
2016-2025
Institute of Molecular Biology and Genetics
2001-2024
International Vaccine Institute
2001-2002
Samsung (South Korea)
2001
Hanyang University
2001
Stanford University
1988-1998
Dankook University
1997
Stanford Medicine
1988
Srg3 (SWI3-related gene product) is a mouse homolog of yeast SWI3,Drosophila melanogaster MOIRA (also named MOR/BAP155), and human BAF155 known as core subunit SWI/SNF complex. This complex involved in the chromatin remodeling required for regulation transcriptional processes associated with development, cellular differentiation, proliferation. We generated mice null mutation theSrg3 locus to examine its function vivo. Homozygous mutants develop early implantation stage but undergo rapid...
Systemic low-grade chronic inflammation has been intensively investigated in obese subjects. Recently, various immune cell types, such as macrophages, granulocytes, helper T cells, cytotoxic and B have implicated the pathogenesis of adipose tissue inflammation. However, roles invariant natural killer cells (iNKT cells) regulation iNKT activity are not thoroughly understood. Here, we demonstrated that were decreased number Interestingly, CD1d, a molecule involved lipid antigen presentation to...
AbstractWe have previously described a SWI/SNF-related protein complex (PYR complex) that is restricted to definitive (adult-type) hematopoietic cells and specifically binds DNA sequences containing long stretches of pyrimidines. Deletion an intergenic DNA-binding site for this from human β-globin locus construct results in delayed γ- switching transgenic mice, suggesting the PYR acts facilitate switch. We now show activity also copurifies with subunits second type chromatin-remodeling...
Neurogenesis is a key developmental event through which neurons are generated from neural stem/progenitor cells. Chromatin remodeling BAF (mSWI/SNF) complexes have been reported to play essential roles in the neurogenesis of central nervous system. However, whether required for neuron generation olfactory system unknown. Here, we identified onscBAF and ornBAF complexes, specifically present stem cells (oNSCs) receptor (ORNs), respectively. We demonstrated that BAF155 subunit highly expressed...
Metabolic dysfunction-associated steatotic liver disease (MASLD) is characterized by fat accumulation in the liver. MASLD encompasses both steatosis and MASH. Since MASH can lead to cirrhosis cancer, must be distinguished during patient treatment. Here, we investigate genomes, epigenomes, transcriptomes of patients identify signature gene set for more accurate tracking progression.
To understand the tumor-suppressing mechanism of SWI/SNF chromatin remodeling complex, we investigated its molecular relationship with p53. Using pREP4-luc episomal reporter, first demonstrated that p53 utilizes activity complex to initiate transcription from chromatin-structured promoter. Among components identified BAF60a as a mediator interaction by yeast two-hybrid assay. directly interacted only BAF60a, but not other such BRG1, SRG3, SNF5, or BAF57. We found out multiple residues at...
The mammalian SWI/SNF complex is an evolutionarily conserved ATP-dependent chromatin remodeling that consists of nine or more components. SRG3, a murine homologue yeast SWI3, Drosophila MOIRA, and human BAF155, core component the required for regulation transcriptional processes associated with development, cellular differentiation, proliferation. Here we report SRG3 interacts directly other components such as SNF5, BRG1, BAF60a. SWIRM domain SANT were SRG3-SNF5 SRG3-BRG1 interactions,...
Abstract During the process of B cell development, transcription factors, such as E2A and Ebf1, have been known to play key roles. Although factors chromatin regulators work in concert direct expression lineage-specific genes, little is about involvement for structure during lymphopoiesis. In this article, we show that deletion Srg3/mBaf155, a scaffold subunit SWI/SNF-like BAF complex, hematopoietic lineage caused defects at both common lymphoid progenitor stage transition from pre–pro-B...
During early cortical development, neural stem cells (NSCs) divide symmetrically to expand the progenitor pool, whereas, in later stages, NSCs asymmetrically self-renew and produce other cell types. The timely switch from such proliferative differentiative division critically determines neuron numbers. However, mechanisms that limit late development are not fully understood. Here, we show BAF (mSWI/SNF) complexes restrict competence promote neuronal differentiation corticogenesis....
Dynamic control of gene expression is essential for development a totipotent zygote into an embryo with defined cell lineages. The accessibility genes responsible specification to transcriptional machinery dependent on chromatin remodelling complexes such as the SWI\SNF (BAF) complex. However, role BAF complex in early mouse has remained unclear. Here we demonstrate that BAF155, major subunit, regulates assembly vivo, and lineage blastocyst. We find associations BAF155 other subunits become...
Dermal fibroblasts lose stem cell potency after birth, which prevents regenerative healing. However, the underlying intracellular mechanisms are largely unknown. We uncover postnatal maturation of papillary (PFs) driven by extensive Twist2-mediated remodeling chromatin accessibility. A loss ability PFs occurs with decreased H3K27ac levels. Single-cell transcriptomics, assay for transposase-accessible sequencing (ATAC-seq), and immunoprecipitation (ChIP-seq) reveal trajectory associated in...
The survival of motor neuron (SMN) protein is a major component the pre-mRNA splicing machinery and required for RNA metabolism. Although SMN has been considered fundamental gene central nervous system, due to its relationship with neuromuscular diseases, such as spinal muscular atrophy, recent studies have also revealed requirement in non-neuronal cells peripheral regions. Here, we report that fibro-adipogenic progenitor subpopulation expressing Dpp4 (Dpp4+ FAPs) system. Furthermore, reveal...
The Daudi cell line is a B-lymphoblastoid derived from Burkitt lymphoma. cells lack surface expression of class I HLA molecules despite the presence intracellular heavy chains. They have defect in gene encoding beta 2-microglobulin (beta 2m), resulting translatable mRNA for this protein. It has been thought that deficiency responsible expression. However, data recently presented demonstrating at least one mouse chain can be expressed on absence 2m. These results raised questions whether 2m...
Toll-like receptor (TLR) signaling is tightly controlled to protect hosts from microorganisms while simultaneously preventing uncontrolled immune responses. Tumor necrosis factor receptor-associated 6 (TRAF6) a critical mediator of TLR signaling, but the precise mechanism how TRAF6 protein stability strictly still remains obscure. We show that myeloid-specific deletion inositol polyphosphate multikinase (IPMK), which has both kinase activities and noncatalytic functions, protects mice...
The abundance of basal progenitors (BPs), radial glia (bRGs) and intermediate (bIPs), in primate brain has been correlated to the high degree cortical folding. Here we examined role BAF155, a subunit chromatin remodeling BAF complex, generation progenitor heterogeneity. conditional deletion BAF155 led diminished bIP pool increased number bRGs, due delamination apical RGs. We found that is required for normal activity neurogenic transcription factor PAX6, thus controlling expression genes are...
Exhausted CD8 T cells during chronic inflammatory responses against viral infections and cancer are phenotypically functionally heterogeneous. In particular, with cytolytic effector function have been recently identified among the exhausted cell subsets. However, regulation of their differentiation remains largely unknown. Here, we report that Krüppel-like factor 4 (KLF4) is a critical regulator exhaustion process, promoting cells. KLF4-expressing in contexts showed features transitory...
During exercise, skeletal muscle is exposed to a low oxygen condition, hypoxia. Under hypoxia, the transcription factor hypoxia-inducible factor-1α (HIF-1α) stabilized and induces expressions of its target genes regulating glycolytic metabolism. Here, using muscle-specific gene ablation mouse model, we show that Brg1/Brm-associated 155 (Baf155), core subunit switch/sucrose non-fermentable (SWI/SNF) complex, essential for HIF-1α signaling in muscle. Muscle-specific Baf155 increases oxidative...
Significance T H 17 cells are a subset of CD4 + helper that secrete the cytokine IL-17 and play role in autoimmunity. RORγt is identified as key transcription factor driving differentiation. Sequence analysis indicated contains several conserved DNA-binding domain isotype-specific we termed modulation (TMD). We designed novel therapeutics, tRORγt-TMD, to deliver RORγt-TMD efficiently into nucleus regulates cell functions 17-mediated autoimmune diseases. With same concept, tTbet-TMD also can...
Dysfunction of Th17 cells leads to chronic inflammatory disorders. STAT3 orchestrates the expression proinflammatory cytokines and pathogenic cell differentiation from IL-17-producing cells. However, pathways mediated by signaling are not fully understood. Here, we observed that FRA1 JUNB directly involved in binding sites promoters Fosl1 Junb. Promoter increased IL-17 development Overexpression Fra1 Junb mice resulted susceptibility collagen-induced arthritis (CIA) an increase numbers...