A5055846813- Rheumatoid Arthritis Research and Therapies
- Chromatin Remodeling and Cancer
- Immune Response and Inflammation
- Bone and Joint Diseases
- Cancer Mechanisms and Therapy
- Bone Metabolism and Diseases
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Systemic Sclerosis and Related Diseases
- Ferroptosis and cancer prognosis
- Spondyloarthritis Studies and Treatments
- Osteoarthritis Treatment and Mechanisms
- Gout, Hyperuricemia, Uric Acid
- Autoimmune and Inflammatory Disorders Research
- interferon and immune responses
- Tracheal and airway disorders
- Virus-based gene therapy research
- Connective Tissue Growth Factor Research
- Nuclear Receptors and Signaling
- Ubiquitin and proteasome pathways
- Reproductive Biology and Fertility
- Genomics and Chromatin Dynamics
- Oral microbiology and periodontitis research
- Peptidase Inhibition and Analysis
Pusan National University
2016-2024
Pusan National University Yangsan Hospital
2024
University of Ulsan
2022
Asan Medical Center
2022
Ulsan College
2022
Stanford University
2012-2021
VA Palo Alto Health Care System
2012-2021
Weatherford College
2020
Seoul National University
2001-2012
Geriatric Research Education and Clinical Center
2012
Abstract Introduction Osteoarthritis (OA) is a degenerative disease characterized by cartilage breakdown in the synovial joints. The presence of low-grade inflammation OA joints receiving increasing attention, with synovitis shown to be present even early stages disease. How arises unclear, but proteins fluid affected could conceivably contribute. We therefore surveyed and assessed their immunostimulatory properties. Methods used mass spectrometry survey patients knee OA. multiplex...
Interleukin-34 (IL-34) is a recently defined cytokine, showing functional overlap with macrophage colony stimulating factor (M-CSF). This study was undertaken to address the expression of IL-34 in rheumatoid arthritis (RA) patients and investigate its regulation pathogenic role RA.IL-34 levels were determined RA synovium, synovial fluid (SF) fibroblast-like cells (FLS) by immunohistochemistry, real-time PCR, enzyme-linked immunosorbent assay immunoblotting. activity assessed using Disease...
Objective Anti–citrullinated protein antibodies (ACPAs) are characteristic of rheumatoid arthritis (RA). However, their presence years before the onset clinical RA is perplexing. Although multiple putative citrullinated antigens have been identified, no studies demonstrated specific capacity these to initiate inflammatory arthritis. This study was undertaken recapitulate transition from preclinical and demonstrate local citrullination facilitate this transition. Methods We performed...
The aim was to investigate the effects of nicotine on neutrophil extracellular traps (NETs) formation in current and non-smokers a murine model RA.We compared spontaneous phorbol 12-myristate 13-acetate-induced NETosis between by DNA release binding. Nicotine-induced from assessed binding, NET-specific (myeloperoxidase (MPO)-DNA complex) ELISA real-time fluorescence microscopy. We also used immunofluorescent staining detect nicotinic acetylcholine receptors (nAChRs) neutrophils performed...
Osteoarthritis (OA) is the leading cause of joint failure, yet underlying mechanisms remain elusive, and no approved therapies that slow progression exist. Dysregulated integrin function was previously implicated in OA pathogenesis. However, roles αVβ3 integrin-associated receptor CD47 largely unknown. Here, transcriptomic proteomic analyses human murine osteoarthritic tissues revealed dysregulated expression αVβ3, CD47, their ligands. Using genetically deficient mice pharmacologic...
To understand the tumor-suppressing mechanism of SWI/SNF chromatin remodeling complex, we investigated its molecular relationship with p53. Using pREP4-luc episomal reporter, first demonstrated that p53 utilizes activity complex to initiate transcription from chromatin-structured promoter. Among components identified BAF60a as a mediator interaction by yeast two-hybrid assay. directly interacted only BAF60a, but not other such BRG1, SRG3, SNF5, or BAF57. We found out multiple residues at...
The mammalian SWI/SNF complex is an evolutionarily conserved ATP-dependent chromatin remodeling that consists of nine or more components. SRG3, a murine homologue yeast SWI3, Drosophila MOIRA, and human BAF155, core component the required for regulation transcriptional processes associated with development, cellular differentiation, proliferation. Here we report SRG3 interacts directly other components such as SNF5, BRG1, BAF60a. SWIRM domain SANT were SRG3-SNF5 SRG3-BRG1 interactions,...
Although ankylosing spondylitis (AS) is driven by immune-mediated processes, little known about the presence and role of autoantibodies in this disease. This study was undertaken to investigate whether occur are involved AS.We performed human protein microarray analysis sera derived from patients with AS or other autoimmune disorders identify associated specifically AS, identified autoantibody targeting phosphatase magnesium-dependent 1A (PPM1A) AS. We enzyme-linked immunosorbent assay...
Most previous research investigating osteoporosis in rheumatoid arthritis (RA) has focused on female patients and there is a lack of data regarding clinical characteristics male with RA. The aim this study was to compare the frequency between RA healthy patients, identify risk factors for low bone mineral density (BMD) We conducted retrospective, cross-sectional including 76 South Korean aged over 50 years age-matched individuals. BMD measured at lumbar spine (L1-4) left hip (femoral neck...
Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by skin and internal organ fibrosis obliterative vasculopathy. Few effective treatments are currently available for in SSc, therefore, demand persists novel therapies. Although use of
In oriental medicine, centipede Scolopendra subspinipes mutilans has long been used as a remedy for rheumatoid arthritis (RA), well-known chronic autoimmune disorder. However, the molecular identities of its bioactive components have not yet extensively investigated. We sought to identify molecules that control RA with centipede. A novel antimicrobial peptide (AMP) (scolopendrasin IX) was identified from mutilans. Scolopendrasin IX markedly activated mouse neutrophils, by enhancing cytosolic...
Abstract Background The pathophysiology of osteoarthritis (OA) involves wear and tear, a state low-grade inflammation. Tissue repair responses include transforming growth factor beta (TGFβ)-induced myofibroblast production extracellular matrix. Fibronectins are an essential part the matrix, injection fibronectin fragments into rabbit joints is previously established animal model OA. Fibronectin containing ED-A domain currently being used as drug delivery target in development...
Insulin plays a critical role in whole-body energy homeostasis by regulating lipid and glucose metabolism. In fat liver tissues, ADD1/SREBP1c is key transcription factor to mediate insulin-dependent regulation of gene expression. Although transcriptional proteolytic activation has been studied intensively, the mechanism which insulin regulates expression its target genes with at chromatin level unclear. Here, we reveal that SWI/SNF remodeling factors interact actively regulate enhanced...
Abstract Background Periodontitis is a major inflammatory disease of the oral mucosa that not limited to cavity but also has systemic consequences. Although importance chronic periodontitis been emphasized, immune response induced by and its therapeutic effects remain elusive. Here, we report transcriptomes peripheral blood mononuclear cells (PBMCs) from patients with periodontitis. Methods Using single-cell RNA sequencing, profiled PBMCs healthy controls paired pre- post-treatment We...
The WAVE regulatory complex (WRC) is involved in various cellular processes by regulating actin polymerization. dysregulation of WRC components associated with cancer development. ABI family member 3 (ABI3)/new molecule including SH3 (NESH) one the and it has been reported that ABI3 phosphorylation can affect function. Although several residues have to be possible sites, still unclear which are important for function ABI3. Furthermore, how phosphorylated form regulated. Here, we demonstrate...
Abstract Immature double-positive thymocytes are sensitive to glucocorticoid (GC)-induced apoptosis, whereas mature single-positive T cells relatively resistant. Thymocytes seem acquire resistance GCs during differentiation into thymocytes. However, detailed knowledge concerning what determines the sensitivity of and how GC is regulated in development lacking. We have previously reported that murine SRG3 gene (for SWI3-related gene) required for GC-induced apoptosis a thymoma cell line....
Although the positive correlation between serum uric acid (UA) levels and bone mineral density (BMD) has been reported in general population, there are little data regarding effect of UA on loss patients with rheumatoid arthritis (RA).We investigated whether increased were associated a reduced risk osteoporosis postmenopausal women RA.In this retrospective cross-sectional study, 447 female RA 200 age-matched, healthy controls underwent BMD examination by dual energy x-ray absorptiometry...