A5034643609- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- Rheumatoid Arthritis Research and Therapies
- Monoclonal and Polyclonal Antibodies Research
- NF-κB Signaling Pathways
- Spondyloarthritis Studies and Treatments
- Trace Elements in Health
- Systemic Lupus Erythematosus Research
- Psoriasis: Treatment and Pathogenesis
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- Heavy Metal Exposure and Toxicity
- IL-33, ST2, and ILC Pathways
- Pharmacological Effects of Natural Compounds
- Autoimmune and Inflammatory Disorders Research
- Bone Metabolism and Diseases
- Immunodeficiency and Autoimmune Disorders
- Diabetes and associated disorders
- Asthma and respiratory diseases
- interferon and immune responses
- Iron Metabolism and Disorders
- Natural product bioactivities and synthesis
- Clostridium difficile and Clostridium perfringens research
Hanyang University
2016-2025
Anyang University
2025
Institute of Cell Biology and Neurobiology
1998-2025
Seoul St. Mary's Hospital
2000-2024
Catholic University of Korea
1999-2023
Institute of Biomedical Science
2009
Bipar
1999-2003
Vanderbilt University
1998-2001
University of Connecticut
1993-1999
Abstract Class-switched IgG autoantibodies but not unswitched IgM play a crucial role in the development of systemic lupus erythematosus (SLE). Bach2 is known to be essential for class switch recombination Ig genes, recent genomic and clinical studies have suggested an association deficiency with SLE. This study was undertaken examine mechanism by which regulates Despite defects germinal center formation when actively immunized, −/− mice spontaneously accumulated autoantibody-secreting cells...
Abstract Objective Poly(lactic‐co‐glycolic acid) (PLGA), a biodegradable polymer, is carrier for drug delivery systems. This study was undertaken to investigate the tolerogenic effect of single administration PLGA entrapping type II collagen (CII) on development collagen‐induced arthritis (CIA). Methods The biophysical properties nanoparticles CII (PLGA‐CII) were investigated by in vitro release testing CII, immunohistochemistry analysis, and electron microscopy. PLGA‐CII fed singly animals...
Abstract Objective Bone destruction is a critical pathology involved in the functional disability caused by rheumatoid arthritis (RA). Osteoclasts, which are specialized bone‐resorbing cells regulated cytokines such as RANKL, implicated bone RA. The aim of this study was to determine whether interleukin‐21 (IL‐21), potent immunomodulatory 4–α‐helical bundle type 1 cytokine, has osteoclastogenic activity patients with RA and mice collagen‐induced (CIA). Methods expression IL‐21 synovial...
Abstract Rheumatoid arthritis is a joint-specific autoimmune inflammatory disease of unknown etiology. The K/BxN mouse model rheumatoid that thought to be mainly due autoantibody-mediated responses. We showed previously homeostatic proliferation autoreactive CD4+ T cells required for initiation in the mice. In this study, we show homeostatically proliferating CD4+CD25− produce IL-21. generated IL-21R-deficient (IL-21R−/−) mice and found these were completely refractory development...
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of autoantibodies to components cell nucleus. These are predominantly produced with help follicular/helper T (Tfh) cells and form immune complexes that trigger widespread inflammatory damage, including nephritis. In recent studies, mesenchymal stem (MSCs) elicited diverse, even opposing, effects in experimental clinical SLE. Here we investigated effect human bone marrow-derived MSCs (hBM-MSCs) a...
Follicular helper T (TFH) cells are recently highlighted as their crucial role for humoral immunity to infection well abnormal control induce autoimmune disease. During an infection, naïve differentiating into TFH which mediate memory B and long-lived plasma in germinal center (GC). characterized by expression of master regulator, Bcl-6, chemokine receptor, CXCR5, essential the migration cell follicle. Within follicle, crosstalk occurs between cells, leading class switch recombination...
Abstract Systemic lupus erythematosus (SLE) is mediated by a chronic and dysregulated inflammatory response. Interleukin (IL)-17, proinflammatory cytokine, T helper (Th)17 cells are associated with autoimmune diseases. We hypothesized that inhibition of IL-17 would decrease the numbers cell subsets function as B-cell helpers, well differentiation into plasma autoantibody expression. The level was increased markedly in Roquin san/san mice. Loss mice improved nephritis downregulating...
Since NEK7 is critical for NLRP3 inflammasome activation, inhibitors could be employed as therapeutic agents against gout, a representative disease caused by inflammasome. We designed based on biochemical kinome profiling of 2,7-substituted thieno[3,2-d]pyrimidine derivatives (SLC3031~3035 and SLC3037). Inflammasome activation was assessed ELISA IL-1b immunoblotting maturation after treatment bone marrow-derived macrophages with LPS+monosodium urate (MSU). NLPR3 binding to oligomerization...
Many studies on osteoblasts have suggested that Wnt5a plays a crucial role in excessive osteoblast activity, which is responsible for ectopic new bone formation, but research osteoclasts ankylosing spondylitis (AS) remains relatively limited.This study aimed to explore whether influences osteoclastmediated resorption curdlan-injected SKG mice, model mimics AS.Compared the Vehicle group, treatment group exhibited statistically higher clinical arthritis scores and increased hindpaw thickness...
Objective To determine the presence of specific immune recognition type II collagen (CII) and its immunodominant epitope CII (255–274) in patients with rheumatoid arthritis (RA). Methods T cell proliferative responses to bovine a synthetic peptide encompassing peripheral blood mononuclear cells (PBMC) synovial fluid (SFMC) from RA patients, PBMC osteoarthritis (OA) healthy controls were assayed by mixed lymphocyte culture. Results The stimulation index (SI) number positive (SI ≥2) higher (n...
Foxp3(+) regulatory T cells (Tregs) are crucial for maintaining cell tolerance, but their role in humoral autoimmunity remains unclear. To address this, we combined a model of autoantibody-dependent arthritis (K/BxN) with Foxp3 mutant scurfy mice to generate Treg-deficient K/BxN mice, referred as K/BxNsf mice. The disease symptoms were exacerbated, and this coincided increases extrafollicular Th cells, follicular germinal centers. Surprisingly, the exhibited an abnormal accumulation mature...
SUMMARY The aim of this study was to investigate the role IL-12 in patients with RA. (p70) and its associated cytokines were measured sera synovial fluid (SF) using an enzyme-linked immunosorbent method. Seven American College Rheumatology (ACR) core set measures as well levels sequentially monitored at commencement 4 months after treatment a low-dose steroid disease-modifying anti-rheumatic drugs (DMARDs). In sera, 64 (42.2%) 152 RA had detectable concentrations (p70), whereas one (1.4%) 69...
The approximately 20-kDa heat-labile toxin produced by enterotoxigenic Bacteroides fragilis is known to be associated with the development of enteritis. However, molecular mechanism involved not yet fully understood. In this study, we assessed whether B. enterotoxin (BFT)-induced enteritis related mitogen-activated protein kinase (MAPK) signaling pathways. human colon epithelial cells, BFT activated three major MAPK cascades. activation p38 was sustained for a relatively long period, while...
Abstract Objective To determine the antiangiogenic effect of cyclosporin A (CSA) in rheumatoid arthritis (RA). Methods We investigated CSA on production vascular endothelial growth factor (VEGF) by synovial fibroblasts. Fibroblast‐like synoviocytes (FLS) were prepared from tissues RA patients, and cultured presence CSA. The VEGF FLS was measured culture supernatants enzyme‐linked immunosorbent assay. messenger RNA (mRNA) expression activator protein 1 (AP‐1) binding activity for...
SUMMARY Metallothionein is a low molecular weight, cysteine-rich, stress response protein that can act as an antioxidant and immunosuppressive agent in instances of antigen-dependent adaptive immunity. In this context, we assessed the therapeutic potential mechanisms action metallothionein collagen-induced arthritis model. Repeated administration metallothionein-I + II during course disease dramatically reduced incidence severity disease. Joint tissues isolated from boostered paws II-treated...
Abstract Intestinal epithelial cells are known to upregulate the expression of several chemokines in response stimulation with bacterial toxin. However, cellular mechanisms Clostridium difficile toxin A‐induced mucosal inflammation have not yet been fully elucidated. In this study, we investigated whether nuclear factor‐kappa B (NF‐κB) could regulate chemokine intestinal cells. Toxin A increased levels NF‐κB complexes containing p65/p50 heterodimers and p65/p65 homodimers. Concurrently,...
Dysfunction of Th17 cells leads to chronic inflammatory disorders. STAT3 orchestrates the expression proinflammatory cytokines and pathogenic cell differentiation from IL-17-producing cells. However, pathways mediated by signaling are not fully understood. Here, we observed that FRA1 JUNB directly involved in binding sites promoters Fosl1 Junb. Promoter increased IL-17 development Overexpression Fra1 Junb mice resulted susceptibility collagen-induced arthritis (CIA) an increase numbers...
Exposure to environmental toxicants can alter a variety of cellular functions that are critical immune function. Cellular responses these changes include increased synthesis number stress proteins, some which have been shown immunomodulatory capacity. One metallothionein (MT) is low molecular weight, cysteine-rich protein be induced by exposure stressors as well many inflammatory and tumorigenic agents. As consequence, high levels MT found at sites inflammation in certain types neoplastic...