A5032308371- Adipose Tissue and Metabolism
- Sirtuins and Resveratrol in Medicine
- Liver Disease Diagnosis and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Diet, Metabolism, and Disease
- Pancreatic function and diabetes
- Muscle Physiology and Disorders
- Diabetes Treatment and Management
- Genomics, phytochemicals, and oxidative stress
- Immune cells in cancer
- Drug Transport and Resistance Mechanisms
- Cancer, Hypoxia, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Autophagy in Disease and Therapy
- Diet and metabolism studies
- Drug-Induced Hepatotoxicity and Protection
- Exercise and Physiological Responses
- Natural product bioactivities and synthesis
- Calcium signaling and nucleotide metabolism
- Mast cells and histamine
- Cancer, Lipids, and Metabolism
- Peptidase Inhibition and Analysis
- FOXO transcription factor regulation
- Lipid metabolism and biosynthesis
Jeonbuk National University
2019-2025
Woosuk University
2011-2019
Hallym University
2011-2016
Chuncheon Sacred Heart Hospital
2016
University of California, San Diego
2009-2012
Hallym University Medical Center
2012
Seoul National University
1993-2010
Norwegian Cancer Society
1983
Chronic inflammation is an important etiology underlying obesity-related disorders such as insulin resistance and type 2 diabetes, recent findings indicate that the macrophage can be initiating cell responsible for this chronic inflammatory state. The mammalian silent information regulator homolog SIRT1 modulates several physiological processes life span, a potential role of in regulation sensitivity has been shown. However, with respect to inflammation, regulating proinflammatory pathway...
Macrophage-mediated inflammation is a key component of insulin resistance; however, the initial events monocyte migration to become tissue macrophages remain poorly understood. We report new method quantitate in vivo macrophage tracking (i.e., blood monocytes from donor mice) labeled ex with fluorescent PKH26 dye and injected into recipient mice. Labeled appear as adipose, liver, splenic macrophages, peaking 1-2 days. When CCR2 KO are wild-type (WT) recipients, or WT given MCP-1 adipose...
Obesity represents a state of chronic, low grade inflammation and is associated with infiltration increased numbers adipose tissue macrophages (ATMs). Diet-induced obesity leads to an increase in non-inflammatory M1-like ATMs displaying the CD11c surface marker. We assessed function CD11c-positive when insulin resistant high fat diet (HFD) mice become insulin-sensitive after switching from HFD normal chow (NC). rapidly all major insulin-target tissues, including muscle, liver, tissue,...
Obesity-related insulin resistance is closely associated with macrophage accumulation and subsequent cytokine release in local tissues. Sirtuin 6 (Sirt6) known to exert an anti-inflammatory function, but its role macrophages the context of obesity has not been investigated. We generated myeloid-specific Sirt6 knockout (mS6KO) mice investigated metabolic characteristics after high-fat diet (HFD) feeding for 16 weeks. Compared their wild-type littermates, HFD-fed mS6KO exhibited greater...
Prostate cancer (PCa) is the most commonly diagnosed genital in men worldwide. Around 80% of patients who developed advanced PCa suffered from bone metastasis, with a sharp drop survival rate. Despite great efforts, detailed mechanisms underlying castration-resistant (CRPC) remain unclear. Sirtuin 5 (SIRT5), an NAD+-dependent desuccinylase, hypothesized to be key regulator various cancers. However, compared other SIRTs, role SIRT5 has not been extensively studied. Here, we revealed...
Abstract Objective Poly(lactic‐co‐glycolic acid) (PLGA), a biodegradable polymer, is carrier for drug delivery systems. This study was undertaken to investigate the tolerogenic effect of single administration PLGA entrapping type II collagen (CII) on development collagen‐induced arthritis (CIA). Methods The biophysical properties nanoparticles CII (PLGA‐CII) were investigated by in vitro release testing CII, immunohistochemistry analysis, and electron microscopy. PLGA‐CII fed singly animals...
Dithiolethiones, a novel class of adenosine monophosphate-activated protein kinase (AMPK) activators, prevent insulin resistance through AMPK-dependent p70 ribosomal S6 kinase-1 (S6K1) inhibition. There is no known effect S6K1 for liver X receptor-alpha (LXRα)-mediated lipogenic gene expression and steatosis, cause chronic disease. This study investigated the role in LXRα activation effects oltipraz (prototype) other dithiolethiones on LXRα-dependent lipogenesis hepatocytes high-fat diet...
Sirtuin (Sirt)6 has been implicated in negative regulation of inflammation and lipid metabolism, although its function the progression from simple steatosis to nonalcoholic steatohepatitis (NASH) remains be defined. To explore role hepatocyte Sirt6 NASH development, we generated hepatocyte-specific Sirt6-knockout (KO) mice that were fed a high-fat high-fructose (HFHF) diet for 16 wk. HFHF-fed KO had increased hepatic aggravated glucose intolerance insulin resistance compared with wild-type...
Abstract Sirtuin 6 (Sirt6), a chromatin associated class III deacetylase, controls whole-body energy homeostasis and has critical role in glucose-stimulated insulin secretion (GSIS) pancreatic β cells. However, its underlying molecular mechanism remains poorly understood. To gain further insights, we studied the pathway by which Sirt6 regulates GSIS utilizing mice lacking their cells (βS6KO). Further, overexpressed wild type or deacetylase-inactive mutant isolated islets as well MIN6 We...
Enhanced anticancer activity and intracellular uptake of paclitaxel-containing solid lipid nanoparticles in multidrug-resistant breast cancer cells Wenting Xu, Eun Ju Bae, Mi-Kyung Lee Department Pharmaceutical Sciences, Woosuk University, Jeonbuk, South Korea Purpose: The aim this study was to show enhanced paclitaxel (Ptx) incorporated into (SLNs) reveal reversal multidrug resistance (MDR) by SLNs mediated increased through different entry mechanisms from that drug-sensitive...
Abstract Expanding the exercise capacity of skeletal muscle is an emerging strategy to combat obesity-related metabolic diseases and this can be achieved by shifting fibers toward slow-twitch oxidative type. Here, we report that Sirt6, anti-aging histone deacetylase, critical in regulating myofiber configuration type Sirt6 activator mimetic. Genetic inactivation reduced while its transgenic overexpression increased mitochondrial performance mice. Mechanistically, show downregulated Sox6, a...
Sirtuin 2 (Sirt2) is known to negatively regulate anoxia-reoxygenation injury in myoblasts. Because protein levels of Sirt2 are increased ischemia-reperfusion (I/R)-injured liver tissues, we examined whether protective or detrimental against hepatic I/R injury. We overexpressed the C57BL/6 mice using a adenovirus. Wild-type and knockout were subjected partial (70%) ischemia for 45 minutes, followed by various periods reperfusion. In another set experiments, wild-type pretreated...
Abstract The active spliced form of X-box-binding protein 1 (XBP1s) is a key modulator ER stress, but the functional role its post-translational modification remains unclear. Here, we demonstrate that XBP1s deacetylation target Sirt6 and protects against stress-induced hepatic steatosis. Specifically, abundance acetylated concordant steatosis were increased in hepatocyte-specific knockout obese mice decreased by genetic overexpression pharmacological activation Sirt6. Mechanistically,...
We recently reported that myeloid sirtuin 6 (Sirt6) is a critical determinant of phenotypic switching and the migratory responses macrophages. Given prominent role macrophages in pathogenesis rheumatoid arthritis (RA), we tested whether Sirt6 deficiency affects development exacerbation RA.Arthritis was induced wild type knockout (mS6KO) mice using collagen-induced K/BxN serum transfer models. expression (or activity) inflammatory activities were compared peripheral blood mononuclear cells...
We recently reported that myeloid cell-expressed sirtuin 6 (Sirt6) plays a crucial role in M1 macrophage polarization and chemotaxis. Given the prominent of macrophages during wound repair heterogeneity, we hypothesized Sirt6 deficiency cells would delay skin closure by affecting phenotypes wounds. To address this question, full-thickness excisional lesion was made dorsal cell-specific knockout (KO) wild-type mice. Wound delayed KO mice, which exhibited less collagen deposition, suppressed...
Inflammation is an important factor in the development of insulin resistance. SIRT1, a class 3 histone/protein deacetylase, has anti-inflammatory functions. Myeloid-specific deletion Sirt1 promotes macrophage infiltration into insulin-sensitive organs and aggravates tissue inflammation. In this study, we investigated how SIRT1 macrophages alters inflammation pancreas as well liver adipose tissue, further explored role locomotion macrophages. -deleted mice (mS1KO) WT littermates were fed 60%...