A5100711004- Nanopore and Nanochannel Transport Studies
- Microfluidic and Capillary Electrophoresis Applications
- Influenza Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- interferon and immune responses
- Biomedical Text Mining and Ontologies
- Ion-surface interactions and analysis
- Dyeing and Modifying Textile Fibers
- RNA and protein synthesis mechanisms
- Marine and fisheries research
- Cancer-related Molecular Pathways
- Chromosomal and Genetic Variations
- Natural product bioactivities and synthesis
- Respiratory viral infections research
- Parallel Computing and Optimization Techniques
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Bioinformatics and Genomic Networks
- Genomics and Phylogenetic Studies
- Diverse Approaches in Healthcare and Education Studies
- Cell death mechanisms and regulation
- Gene expression and cancer classification
- Steroid Chemistry and Biochemistry
- Microfluidic and Bio-sensing Technologies
Korea Research Institute of Bioscience and Biotechnology
2016-2025
Korea University of Science and Technology
2021-2024
Korea University
2022
Government of the Republic of Korea
2021
Daejeon University
2018-2021
Chung-Ang University
2013-2020
Hanyang University
2016-2018
Korea Advanced Institute of Science and Technology
2012-2018
Woosuk University
2010-2018
Inha University
2017
In drug discovery, efficient screening of protein-drug interactions (PDIs) is hampered by the limitations current biophysical approaches. Here, we develop a biological nanopore sensor for single-molecule detection proteins and PDIs using pore-forming toxin YaxAB. Using this YaxAB nanopore, demonstrate label-free, between anticancer Bcl-xL protein small-molecule drugs as well Bak-BH3 peptide. The long funnel-shaped structure nanofluidic characteristics enable electro-osmotic trapping diverse...
Diabetic heart failure may be causally associated with alterations in cardiac energy metabolism and insulin resistance. Mice heart-specific overexpression of peroxisome proliferator–activated receptor (PPAR)α showed a metabolic cardiomyopathic phenotype similar to the diabetic heart, we determined tissue-specific glucose action vivo during hyperinsulinemic-euglycemic clamps awake myosin heavy chain (MHC)-PPARα mice (12–14 weeks age). Basal insulin-stimulated uptake was significantly reduced...
Conformational transitions and structural rearrangements are central to the function of many RNAs yet remain poorly understood. We have used ultrafast multidimensional NMR techniques monitor adenine-induced folding an adenine-sensing riboswitch in real time, with nucleotide-resolved resolution. By following changes 2D spectra at rates approximately 0.5 Hz, we identify distinct steps associated ligand-induced riboswitch. Following recognition ligand, long range loop-loop interactions form...
Enhanced anticancer activity and intracellular uptake of paclitaxel-containing solid lipid nanoparticles in multidrug-resistant breast cancer cells Wenting Xu, Eun Ju Bae, Mi-Kyung Lee Department Pharmaceutical Sciences, Woosuk University, Jeonbuk, South Korea Purpose: The aim this study was to show enhanced paclitaxel (Ptx) incorporated into (SLNs) reveal reversal multidrug resistance (MDR) by SLNs mediated increased through different entry mechanisms from that drug-sensitive...
Through screening by NMR spectroscopy, we discovered a novel scaffold (DPQ: 6,7-dimethoxy-2-(1-piperazinyl)-4-quinazolinamine) that binds specifically to the influenza A virus RNA promoter. The solution structure of RNA–DPQ complex reported here demonstrates internal loop is binding site DPQ. exhibits antiviral activity against viruses.
Abstract Nanopore sensing is an emerging technology for the single‐molecule‐based detection of various biomolecules. In this study, we probed anticancer therapeutic p53 transactivation domain (p53TAD)/MDM2 interaction and its inhibition with a small‐molecule MDM2 antagonist, Nutlin‐3, using low‐noise solid‐state nanopores. Although translocation positively charged through nanopore was detected at applied negative voltage, almost completely blocked upon formation MDM2/GST‐p53TAD complex owing...
Retinoic acid-inducible gene I (RIG-I) recognizes specific molecular patterns of viral RNAs for inducing type interferon. The C-terminal domain (CTD) RIG-I binds to double-stranded RNA (dsRNA) with the 5′-triphosphate (5′-PPP), which induces a conformational change in an active form. It has been suggested that detects infection influenza A virus by recognizing 5′-triphosphorylated panhandle structure genome. Influenza unique sharp helical bending. In spite extensive studies how activate...
Some bioflavonoids are potent antioxidants and have pharmacological effects similar to those of vitamin E. The interactive effect naringin E was studied with respect cholesterol metabolism antioxidant status. Naringin supplementation (0.1%, wt/wt) comparable levels given rats a high-cholesterol (1%, diet for 5 weeks. amount included in naringin-free diets low (low-E) normal (normal-E) level. significantly lowered the concentrations plasma triglyceride compared group E-fed rats. HMG-CoA...
Nutlin-3-induced conformation change of p53TAD–MDM2 fusion protein were probed by single molecule translocation event using solid-state nanopores.
Neuraminidase (NA), one of the major surface glycoproteins influenza A virus (IAV), is an important diagnostic biomarker and antiviral therapeutic target. Cytolysin (ClyA) a nanopore sensor with internal constriction 3.3 nm, enabling detection protein conformations at single-molecule level. In this study, nanopore-based approach developed for analysis enzymatic activity NA, which facilitates rapid highly sensitive diagnosis IAV. Current blockade d-glucose/d-galactose-binding (GBP) trapped...
Nanopore sensors are a highly attractive platform for single-molecule sensing sequencing, disease diagnostics, and drug screening. Outer membrane protein G (OmpG) nanopores have advantages owing to their rigid monomeric structure, which comprises seven flexible loops, providing distinct gating patterns upon analyte binding. Blocking of the protein–protein interaction between B-cell lymphoma-extra-large (Bcl-xL) BH3 domain Bcl-2 homologous antagonist/killer (Bak-BH3) has been reported as...
Terfenadine, an antihistaminic drug, has relatively low bioavailability after oral administration due to its limited solubility in water. To enhance the activity of terfenadine, terfenadine–β-cyclodextrin (1 : 2) inclusion complex was prepared by neutralization method. The and dissolution were carried out, then evaluated compared with terfenadine powder passive subcutaneous anaphylaxis method rats. formation constant higher at lower pH, while ratio 1 2 irrespective pH. For it improved 200...
Bacterial riboswitch RNAs are attractive targets for novel antibiotics against antibiotic-resistant superbacteria. Their binding to cognate metabolites is essential the regulation of bacterial gene expression. Despite importance as therapeutic targets, development RNA-targeted, small-molecule drugs limited by current biophysical methods. Here, we monitored specific interaction between adenine-sensing aptamer domain (ARS) and adenine at single-molecule level using α-hemolysin (αHL) nanopores....