A5100643048- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Plant-Microbe Interactions and Immunity
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Cancer Research and Treatments
- Bacterial biofilms and quorum sensing
- Diverse Topics in Contemporary Research
- Enzyme Production and Characterization
- Nanoplatforms for cancer theranostics
- Foot and Ankle Surgery
- Microbial Community Ecology and Physiology
- Consumer Perception and Purchasing Behavior
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Orthopedic Surgery and Rehabilitation
- Diverse Approaches in Healthcare and Education Studies
- Virus-based gene therapy research
- Insect Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- ZnO doping and properties
- Oral microbiology and periodontitis research
- Tendon Structure and Treatment
- Mycotoxins in Agriculture and Food
Korea Research Institute of Bioscience and Biotechnology
2016-2025
Chung-Ang University
2015-2023
Chung-Ang University Hospital
2021-2023
Hanwha Solutions (South Korea)
2021
University of Ulsan
2019-2020
Ulsan College
2019-2020
Korea University of Science and Technology
2010-2020
Asan Medical Center
2019-2020
Ulsan University Hospital
2020
Korea Center for Disease Control and Prevention
2019
Engineered Salmonella secreting heterologous bacterial flagellin suppress tumor growth by activating intratumoral macrophages.
We propose a bacteria-based microrobot (bacteriobot) based on new fusion paradigm for theranostic activities against solid tumors. develop bacteriobot using the strong attachment of bacteria to Cy5.5-coated polystyrene microbeads due high-affinity interaction between biotin and streptavidin. The chemotactic responses bacteriobots concentration gradients lysates or spheroids tumors can be detected as migration and/or out central region toward side regions in microfluidic chamber. showed...
ABSTRACT Polymyxin, a long-known peptide antibiotic, has recently been reintroduced in clinical practice because it is sometimes the only available antibiotic for treatment of multidrug-resistant gram-negative pathogenic bacteria. Lack information on biosynthetic genes polymyxin, however, limited study structure-function relationships and development improved polymyxins. During whole genome sequencing Paenibacillus polymyxa E681, plant growth-promoting rhizobacterium, we identified gene...
Bacteria-based anticancer therapies aim to overcome the limitations of current cancer therapy by actively targeting and efficiently removing cancer.To achieve this goal, new approaches that target maintain bacterial drugs at sufficient concentrations during therapeutic window are essential.Here, we examined tumor tropism attenuated Salmonella typhimurium displaying RGD peptide sequence (ACDCRGDCFCG) on external loop outer membrane protein A (OmpA).RGD-displaying strongly bound cells...
A number of recent reports have demonstrated that attenuated Salmonella typhimurium are capable targeting both primary and metastatic tumors. The use bacteria as a vehicle for the delivery anticancer drugs requires mechanism precisely regulates visualizes gene expression to ensure appropriate timing location drug production. To integrate these functions into bacteria, we used repressor-regulated tetracycline efflux system, in which therapeutic an imaging reporter were controlled by divergent...
Akkermansia muciniphila is a commensal bacterium using mucin as its sole carbon and nitrogen source. A. promising candidate for next-generation probiotics to prevent inflammatory metabolic disorders, including diabetes obesity, increase the response cancer immunotherapy. In this study, comparative pan-genome analysis was conducted investigate genomic diversity evolutionary relationships between complete genomes of 27 strains, KGMB strains isolated from healthy Koreans. The showed that formed...
Bacterial cancer therapies aim to manipulate bacteria effectively deploy therapeutic payloads tumors. Attenuated alone often cannot eradicate solid Salmonella can be engineered deliver cytotoxic drugs either trigger an immune response or increase antitumor efficacy when combined with chemotherapeutic drugs. However, the extracellular matrix (ECM) surrounding cells forms a barrier that limits ability of and penetrate eliminate To overcome this limitation, we developed strategy combine...
ABSTRACT Paenibacillus polymyxa E681, a spore-forming, low-G+C, Gram-positive bacterium isolated from the rhizosphere of winter barley grown in South Korea, has great potential for agricultural applications due to its ability promote plant growth and suppress diseases. Here we present complete genome sequence P. E681. Its 5.4-Mb encodes functions specialized plant-associated lifestyle characteristics that are beneficial plants, such as production hormone, antibiotics, hydrolytic enzymes.
In B. subtilis, large genomic deletions have been carried out for genome reduction, antibiotic overproduction, and heterologous protein overexpression. view of the eco-friendliness it is critical that engineering preserves its food-grade status avoids leaving foreign DNA in genome. Existing methods generating leave resistance markers or display low mutation efficiency. this study, we introduced a clustered regularly interspaced short palindromic repeat (CRISPR)-derived technique to develop...
Many bacterial species are known to thrive within plants. Among these bacteria, a group referred as endophytes provide beneficial effects the host plants by promotion of plant growth and suppression pathogens. 44 putative endophytic isolates isolated from surface-sterilized rice roots, Burkholderia sp. KJ006 was selected for further study because lack pathogenicity rice, broad spectrum antifungal properties, presence nifH gene, which is an indicator nitrogen fixation. In attempt control...
Bacillus siamensis KCTC 13613(T), a novel halophilic species isolated from salted Thai food, produced antimicrobial compounds against plant pathogens and promoted growth by volatile emission. We determined the 3.8-Mb genome sequence of B. 13613(T) to reveal plant-beneficial effect at genomic level.
ABSTRACT Endophytes live inside plant tissues without causing any harm and may even benefit plants. Here, we provide the high-quality genome sequence of Burkholderia sp. strain KJ006, an endophytic bacterium rice with antifungal activity. The 6.6-Mb genome, consisting three chromosomes a single plasmid, contains genes related to growth promotion or degradation aromatic compounds.
In this study, a new type of targeted bacteriobots is prepared and investigated as therapeutic strategy against solid tumors. Maleimide‐functionalized hyaluronic acid (HA) polymer synthesized cross‐linked with four‐arm‐thiolated polyethylene glycol (PEG‐SH) to form HA microbeads diameter 8 μm through the Michael‐type addition. Docetaxel (DTX)‐loaded nanoparticles are encapsulated in HA–PEG sustained vitro drug‐release pattern DTX from observed for up 96 h. Dual‐targeted using CD 44...
The gut microbiota (GM) has been shown to be closely associated with the development of colorectal cancer (CRC). However, involvement GM is CRC mainly demonstrated by metagenomic profiling studies showing compositional difference between healthy individuals and that patients not directly studying isolated microbes. Thus, discover novel microbes involved in CRC, we from feces evaluated its anti-CRC activity vitro vivo. After isolation, cell-free supernatants (CFSs) were prepared...
The gut microbiota (GM) is associated with colorectal cancer (CRC) development. However, studies demonstrating the role of GM in CRC are limited to metagenomic analyses. These lack direct evidence proving that candidate strains involved CRC, and isolated probiotics for bacteriotherapy. Therefore, identify novel anti-CRC activity, we previously bacteria from feces healthy individuals, screened GM's discovered cell-free supernatants isolates demonstrated antiproliferative activity against...