A5103056397- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- interferon and immune responses
- T-cell and B-cell Immunology
- Cell Image Analysis Techniques
- Image Processing Techniques and Applications
- Cytokine Signaling Pathways and Interactions
- Digital Holography and Microscopy
- Immune cells in cancer
- Inflammasome and immune disorders
- IL-33, ST2, and ILC Pathways
- Influenza Virus Research Studies
- Epigenetics and DNA Methylation
- Particle Detector Development and Performance
- CAR-T cell therapy research
- NF-κB Signaling Pathways
- Mast cells and histamine
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Vectors
- Dark Matter and Cosmic Phenomena
- RNA Interference and Gene Delivery
- Radiation Therapy and Dosimetry
- Acute Myeloid Leukemia Research
- Glycosylation and Glycoproteins Research
Korea Advanced Institute of Science and Technology
2016-2025
Kyungpook National University
2019-2022
Howard Hughes Medical Institute
2002-2009
Yale University
2002-2009
University of California, San Francisco
2006-2009
The San Raffaele Telethon Institute for Gene Therapy
2004
Over the past decade, mesoporous silica nanoparticles (MSNs) smaller than 200 nm with a high colloidal stability have been extensively studied for systemic drug delivery. Although small molecule delivery via MSNs has successful, encapsulation of large therapeutic biomolecules, such as proteins or DNA, is limited due to pore size conventional obtained by soft-templating. Here, we report synthesis extra-large pores (XL-MSNs) and their application in vivo cytokine macrophage polarization....
A synergistic application of holography and deep learning enables rapid optical screening anthrax spores other pathogens.
Abstract During cancer immunoediting, loss of major histocompatibility complex class I (MHC-I) in neoplasm contributes to the evasion tumours from host immune system. Recent studies have demonstrated that most natural killer (NK) cells are found advanced cancers defective, releasing malignant MHC-I-deficient NK-cell-dependent control. Here, we show a T (NKT)-cell-ligand-loaded tumour-antigen expressing antigen-presenting cell (APC)-based vaccine effectively eradicates these tumours. this...
Identification of lymphocyte cell types are crucial for understanding their pathophysiological roles in human diseases. Current methods discriminating primarily rely on labelling techniques with magnetic beads or fluorescence agents, which take time and have costs sample preparation may also a potential risk altering cellular functions. Here, we present the identification non-activated at single-cell level using refractive index (RI) tomography machine learning. From measurements...
CD1d molecules bind lipid antigens in the endocytic pathway, and access to pathway is important for development of CD1d-restricted natural killer T (NKT) cells. Saposins, derived from a common precursor, prosaposin, are small, heat-stable lysosomal glycoproteins required degradation sphingolipids. Expression prosaposin efficient binding recognition human by NKT Despite high sequence homology among four saposins, they have different specificities substrates mechanisms action. To determine...
Abstract Homeostasis of neutrophils—the blood cells that respond first to infection and tissue injury—is critical for the regulation immune responses regulated through granulopoiesis, a multi-stage process by which neutrophils differentiate from hematopoietic stem cells. Granulopoiesis is highly dynamic altered in certain clinical conditions, such as pathologic iatrogenic neutropenia, described demand-adapted granulopoiesis. The granulopoiesis under stress not completely understood because...
Rapid identification of bacterial species is crucial in medicine and food hygiene. In order to achieve rapid label-free at the single bacterium level, we propose experimentally demonstrate an optical method based on Fourier transform light scattering (FTLS) measurements statistical classification. For individual rod-shaped bacteria belonging four (Listeria monocytogenes, Escherichia coli, Lactobacillus casei, Bacillus subtilis), two-dimensional angle-resolved maps are precisely measured...
Members of the CD1 family membrane glycoproteins can present antigenic lipids to T lymphocytes. Like major histocompatibility complex class I molecules, they form a heterodimeric heavy chain and β2-microglobulin (β2m) in endoplasmic reticulum (ER). Binding lipid antigens, however, takes place endosomal compartments, similar II on plasma membrane. Unlike or CD1b which need β2m exit ER, CD1d be expressed cell surface as either free associated with β2m. These differences led us investigate...
Abstract IL-12p40 is induced in macrophages and dendritic cells (DC) after activation by microbial TLR ligands cytokines constitutes a component of IL-12 IL-23. In an effort to understand the location kinetics these during course immune response, we generated knockin (gene-targeted) mice that express p40 gene linked via viral internal ribosome entry site element with fluorescent reporters, eYFP or eGFP. Macrophages DC from faithfully reported biallelic induction using marker. s.c....
CD1d is an MHC class I-like membrane glycoprotein that presents lipid Ags to NKT cells. Despite intensive biochemical, genetic, and structural studies, the endogenous lipids associated with remain poorly defined because of biochemical challenges posed by their hydrophobic nature. In this study, we report generation a protease-cleavable variant similar trafficking pattern wild-type can be purified in absence detergent allows characterization naturally lipids. addition, used soluble variants...
The spatiotemporal regulation of immune cells in lymph nodes (LNs) is crucial for mounting protective T-cell responses, which are orchestrated by dendritic (DCs). However, it unclear how the DC subsets altered inflammatory milieu LNs. Here, we show that inflamed LNs Listeria-infected mice characterized clustering neutrophils and monocytes IFN-γ production. Significantly, early responses coupled with differentiation not one, but two types CD64+CD11c+MHCII+ DCs. Through assessment chemokine...
Abstract Interferons (IFNs) potentiate macrophage activation typically via a STAT1-dependent pathway. Recent studies suggest functioning of STAT1-independent pathway in the regulation gene expression by IFN-γ, thus pointing to diversity cellular responses IFNs. Many functions IFNs rely on cross-regulation exogenous inflammatory mediators such as TLR ligands. Here we investigated contribution and its underlying mechanism context combined stimulation IFN TLR. We found that TLR-induced...
Cells universally adapt to ischemic conditions by turning on a transcription factor hypoxia-inducible (HIF), in which its role is known differ widely across many different types of cells. Given that microglia have been reported as an essential mediator neuroinflammation brain diseases, we examined the HIF progression acute phase stroke challenging our novel strains myeloid-specific Hif-1α or Hif-2α knockout (KO) mice created Cre-loxP system via middle cerebral artery occlusion (MCAO). We...
The CD1 family consists of lipid antigen-presenting molecules, which include group I CD1a, CD1b, and CD1c II CD1d proteins. Topologically, they resemble the classical peptide MHC molecules except that large, exclusively nonpolar hydrophobic, antigen-binding groove has evolved to present cellular pathogen-derived antigens specific T lymphocytes. As an approach understanding biochemical basis antigen presentation by we have characterized natural ligands associated with mouse CD1d1 as well...
Toll-like receptor (TLR) signaling is tightly controlled to protect hosts from microorganisms while simultaneously preventing uncontrolled immune responses. Tumor necrosis factor receptor-associated 6 (TRAF6) a critical mediator of TLR signaling, but the precise mechanism how TRAF6 protein stability strictly still remains obscure. We show that myeloid-specific deletion inositol polyphosphate multikinase (IPMK), which has both kinase activities and noncatalytic functions, protects mice...
Retinoic acid-inducible gene I (RIG-I) recognizes specific molecular patterns of viral RNAs for inducing type interferon. The C-terminal domain (CTD) RIG-I binds to double-stranded RNA (dsRNA) with the 5′-triphosphate (5′-PPP), which induces a conformational change in an active form. It has been suggested that detects infection influenza A virus by recognizing 5′-triphosphorylated panhandle structure genome. Influenza unique sharp helical bending. In spite extensive studies how activate...
Abstract Clonal hematopoiesis of indeterminate potential (CHIP), a common aging-related process that predisposes individuals to various inflammatory responses, has been reported be associated with COVID-19 severity. However, the immunological signature and exact gene expression program by which presence CHIP exerts its clinical impact on remain elucidated. In this study, we generated single-cell transcriptome landscape severe according using peripheral blood mononuclear cells. Patients...
Pancreatic ductal adenocarcinoma is characterized by a three-dimensional (3D) tumor microenvironment devoid of oxygen and nutrients but enriched in extracellular matrix, which acts as physical chemical barrier. In 3D, cancer cells reprogram their metabolic pathways ways that help them survive hostile conditions. However, little known about the phenotypes 3D intrinsic cues modulate them. We found Cxcl5 deletion restricted pancreatic growth spheroid-in-Matrigel culture system without affecting...
The stimulator of IFN genes (STING)-mediated DNA-sensing pathway plays an important role in the innate immune response to pathogen infection, autoimmunity, and cancer; however, its regulatory mechanism has not been fully elucidated, we do yet know whether STING is counter-regulated by other pathways. Here, show that NLRP3-activating agonists, ATP nigericin, prevent activation association with mitochondrial fragmentation; suppression mitochondria fission were dependent on NLRP3 or potassium...