A5013365298- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Influenza Virus Research Studies
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
- Diabetes and associated disorders
- Heparin-Induced Thrombocytopenia and Thrombosis
- Respiratory viral infections research
- Long-Term Effects of COVID-19
- Platelet Disorders and Treatments
- SARS-CoV-2 detection and testing
- Chronic Lymphocytic Leukemia Research
- Nuclear Physics and Applications
- Autoimmune Bullous Skin Diseases
- NF-κB Signaling Pathways
- Intramuscular injections and effects
- CAR-T cell therapy research
- Medical Imaging Techniques and Applications
- Liver Diseases and Immunity
- Drug-Induced Adverse Reactions
- Psoriasis: Treatment and Pathogenesis
Seoul National University
2017-2025
Korea University
2023-2025
Washington University in St. Louis
2020-2024
Institute of Molecular Biology and Genetics
2024
Kyung Hee University
2022-2023
St. Jude Children's Research Hospital
2023
Children's Research Hospital
2023
University School
2023
University of Missouri–St. Louis
2022
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the global COVID-19 pandemic. Rapidly spreading SARS-CoV-2 variants may jeopardize newly introduced antibody and vaccine countermeasures. Here, using monoclonal antibodies (mAbs), animal immune sera, human convalescent sera from recipients of BNT162b2 mRNA vaccine, we report impact on neutralization a panel authentic including B.1.1.7 isolate, chimeric strains with South African or Brazilian spike genes isogenic...
Patients with chronic inflammatory disease (CID) treated immunosuppressive medications have increased risk for severe COVID-19. Although mRNA-based SARS-CoV-2 vaccination provides protection in immunocompetent persons, immunogenicity immunosuppressed patients CID is unclear.To determine the of vaccines CID.Prospective observational cohort study.Two U.S. referral centers.Volunteer sample adults confirmed eligible early COVID-19 vaccination, including hospital employees any age and older than...
ABSTRACT Background Individuals with chronic inflammatory diseases (CID) are frequently treated immunosuppressive medications that can increase their risk of severe COVID-19. While novel mRNA-based SARS-CoV-2 vaccination platforms provide robust protection in immunocompetent individuals, the immunogenicity CID patients on immunosuppression is not well established. Therefore, determining effectiveness vaccines setting essential to risk-stratify impaired and clinical guidance regarding...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the global COVID-19 pandemic infecting more than 106 million people and causing 2.3 deaths. The rapid deployment of antibody-based countermeasures provided hope for curtailing disease ending
Germinal centers (GC) are microanatomical lymphoid structures where affinity-matured memory B cells and long-lived bone marrow plasma primarily generated. It is unclear how the maturation of within GC impacts breadth durability cell responses to influenza vaccination in humans. We used fine needle aspiration draining lymph nodes longitudinally track antigen-specific seasonal vaccination. Antigen-specific persisted for at least 13 wk after two out seven individuals. Monoclonal antibodies...
The primary two-dose SARS-CoV-2 mRNA vaccine series are strongly immunogenic in humans, but the emergence of highly infectious variants necessitated additional doses these vaccines and development new variant-derived ones
Toll-like receptor (TLR) signaling is tightly controlled to protect hosts from microorganisms while simultaneously preventing uncontrolled immune responses. Tumor necrosis factor receptor-associated 6 (TRAF6) a critical mediator of TLR signaling, but the precise mechanism how TRAF6 protein stability strictly still remains obscure. We show that myeloid-specific deletion inositol polyphosphate multikinase (IPMK), which has both kinase activities and noncatalytic functions, protects mice...
Summary SARS-CoV-2 mRNA vaccines induce robust anti-spike (S) antibody and CD4+ T cell responses. It is not yet clear whether vaccine-induced follicular helper (TFH) responses contribute to this outstanding immunogenicity. Using fine needle aspiration of draining axillary lymph nodes from individuals who received the BNT162b2 vaccine, we show that frequency TFH correlates with S-binding germinal center B cells. Mining responding receptor repertoire revealed a strikingly immunodominant...
The differentiation and specificity of human CD4+ T follicular helper cells (TFH cells) after influenza vaccination have been poorly defined. Here we profiled blood draining lymph node (LN) samples from volunteers for over 2 years two vaccines were administered 1 year apart to define the evolution TFH cell response. first induced an increase in frequency circulating (cTFH) LN at week postvaccination. This was transient cTFH cells, whereas further expanded during remained elevated least 3...
Infection or vaccination induces a population of long-lived bone marrow plasma cells (BMPCs) that are persistent and essential source protective antibodies1-5. Whether this is induced in patients infected with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) unknown. Recent reports have suggested SARS-CoV-2 convalescent experience rapid decay their antigen-specific serum antibodies, raising concerns humoral immunity against virus may be short-lived6-8. Here we show who...
Metabolic dysfunction-associated steatohepatitis (MASH) is characterized by severe liver inflammation and fibrosis due to an imbalanced immune response caused enhanced bacterial components. The progression of MASH closely linked increased permeability intestinal mucosal barrier facilitating enter components into hepatic portal venous system. B cells are important for adaptive responses enhance through cytokine production T cell activation. influenced gut microbiota, but the specific...
T cell-independent (TI) B cell response is critical for the early protection against pathogen invasion. The regulation and activation of Bruton's tyrosine kinase (Btk) known as a pivotal step antigen receptor (BCR) signaling in TI humoral immunity, observed patients with X-linked agammaglobulinemia (XLA) experiencing high incidence encapsulated bacterial infections. However, key questions remain to whether well-established canonical BCR pathway sufficient regulate activity Btk. Here, we find...
Abstract Germinal centres (GC) are lymphoid structures where vaccine-responding B cells acquire affinity-enhancing somatic hypermutations (SHM), with surviving clones differentiating into memory (MBCs) and long-lived bone marrow plasma (BMPCs) 1–4 . Induction of the latter is a hallmark durable immunity after vaccination 5 SARS-CoV-2 mRNA induces robust GC response in humans 6–8 , but maturation dynamics propagation their progeny throughout cell diaspora have not been elucidated. Here we...
Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) messenger RNA (mRNA)-based vaccines are ~95% effective in preventing coronavirus disease 2019. However, the dynamics of antibody secreting plasmablasts (PBs) and germinal centre (GC) B cells induced by these SARS-CoV-2 naïve antigen-experienced humans remains unclear. Here we examined peripheral blood and/or lymph node (LN) antigen-specific cell responses 32 individuals who received two doses BNT162b2, an mRNA-based...
Activated Foxp3 + regulatory T (Treg) cells differentiate into effector Treg (eTreg) to maintain peripheral immune homeostasis and tolerance. cell receptor (TCR)–mediated induction regulation of store-operated Ca 2+ entry (SOCE) is essential for eTreg differentiation function. However, SOCE in remains unclear. Here, we show that inositol polyphosphate multikinase (IPMK), which generates tetrakisphosphate pentakisphosphate, a pivotal regulator downstream TCR signaling. IPMK highly expressed...
Objective Little is known regarding the reactogenicity and related SARS–CoV‐2 vaccine response in patients with chronic inflammatory disease (CID). Our objective was to characterize adverse event profile of CID following vaccination understand relationship between immunogenicity vaccines. Methods healthy controls eligible receive messenger RNA (mRNA) vaccines participated 3 study visits (pre‐vaccine, after dose 1, 2) which blood clinical data were collected. Assessment events solicited...