A5064218946- Advanced Fluorescence Microscopy Techniques
- Chromatin Remodeling and Cancer
- Advanced Electron Microscopy Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Microtubule and mitosis dynamics
- Cell Image Analysis Techniques
- Click Chemistry and Applications
- Ion channel regulation and function
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Nonlinear Optical Materials Studies
- Hedgehog Signaling Pathway Studies
- Cardiac electrophysiology and arrhythmias
- bioluminescence and chemiluminescence research
- Nanoplatforms for cancer theranostics
- RNA Research and Splicing
- Receptor Mechanisms and Signaling
- MicroRNA in disease regulation
- 3D Printing in Biomedical Research
- Genomic variations and chromosomal abnormalities
- Photoacoustic and Ultrasonic Imaging
Max Planck Institute for Multidisciplinary Sciences
2022-2024
University of Göttingen
2015-2021
Max Planck Institute for Biophysical Chemistry
2021
Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103
2018
Abstract The development of live-cell fluorescence nanoscopy is powered by the availability suitable fluorescent probes. Rhodamines are among best fluorophores for labeling intracellular structures. Isomeric tuning a powerful method optimizing biocompatibility rhodamine-containing probes without affecting their spectral properties. An efficient synthesis pathway 4-carboxyrhodamines still lacking. We present facile protecting-group-free 4-carboxyrhodamines’ based on nucleophilic addition...
During early cortical development, neural stem cells (NSCs) divide symmetrically to expand the progenitor pool, whereas, in later stages, NSCs asymmetrically self-renew and produce other cell types. The timely switch from such proliferative differentiative division critically determines neuron numbers. However, mechanisms that limit late development are not fully understood. Here, we show BAF (mSWI/SNF) complexes restrict competence promote neuronal differentiation corticogenesis....
The abundance of basal progenitors (BPs), radial glia (bRGs) and intermediate (bIPs), in primate brain has been correlated to the high degree cortical folding. Here we examined role BAF155, a subunit chromatin remodeling BAF complex, generation progenitor heterogeneity. conditional deletion BAF155 led diminished bIP pool increased number bRGs, due delamination apical RGs. We found that is required for normal activity neurogenic transcription factor PAX6, thus controlling expression genes are...
Here we report a small molecule tubulin probe for single-molecule localization microscopy (SMLM), stimulated emission depletion (STED) and MINFLUX nanoscopy, which can be used in living fixed cells. We explored series of taxane derivatives containing spontaneously blinking far-red dye hydroxymethyl silicon–rhodamine (HMSiR) found that the linker length profoundly affects permeability off-targeting The best performing probe, HMSiR-tubulin, is composed cabazitaxel 6′-regioisomer HMSiR bridged...
Abstract Optical imaging access to nanometer-level protein distributions in intact tissue is a highly sought-after goal, as it would provide visualization physiologically relevant contexts. Under the unfavorable signal-to-background conditions of increased absorption and scattering excitation fluorescence light complex medium, super-resolution microscopy methods are severely challenged attaining precise localization molecules. We reasoned that typical use confocal detection pinhole MINFLUX...
Optical imaging access to nanometer-level protein distributions in intact tissue is a highly sought-after goal, as it would provide visualization physiologically relevant contexts. Under the unfavorable signal-to-background conditions of increased absorption and scattering excitation fluorescence light complex sample, superresolution microscopy methods are severely challenged attaining precise localization molecules. We reasoned that typical use confocal detection pinhole MINFLUX nanoscopy,...
To discover new multifunctional agents for the treatment of cardiovascular diseases, we designed and synthesized a series compounds with cyclopropyl alcohol moiety evaluated them in biochemical assays. Biological screening identified derivatives dual activity: preventing Ca2+ leak through ryanodine receptor 2 (RyR2) enhancing cardiac sarco-endoplasmic reticulum (SR) load by activation Ca2+-dependent ATPase 2a (SERCA2a). The that stabilize RyR2 at micro- nanomolar concentrations are either...
Oligodendrocytes are responsible for axon myelination in the brain and spinal cord. Generation of oligodendrocytes entails highly regulated multistage neurodevelopmental events, including proliferation, differentiation maturation. The chromatin remodeling BAF (mSWI/SNF) complex is a notable regulator neural development. In our previous studies, we determined indispensability scaffolding subunits BAF155 BAF170 neurogenesis, whereas their role gliogenesis unknown. Here, show that expression...
Abstract The bacterial bioluminescence system can be applied to produce autonomous in mammalian cells. Until now, the has only been stably inserted into cells cultured vitro . Here we report generation of an autobioluminescent transgenic mouse line constitutively expressing genes system, enabling substrate-free vivo luminescence imaging a living mammal.
Activation of caged fluorophores in microscopy has mostly relied on the absorption a single ultraviolet (UV) photon ≲400 nm wavelength or simultaneous two near-infrared (NIR) photons >700 nm. Here, we show that green (515 nm) can substitute for (~260 to activate popular silicon-rhodamine (Si-R) dyes. range eliminates chromatic aberrations plague activation by UV NIR light. Thus, confocal fluorescence microscopy, focal volume be matched with detection. Besides, detrimental losses and light...
ABSTRACT The development of live-cell fluorescence nanoscopy is powered by the availability suitable fluorescent probes. Rhodamines are among best fluorophores for labeling intracellular structures. Isomeric tuning a powerful method optimizing biocompatibility rhodamine-containing probes without affecting their spectral properties. However, efficient synthesis pathway rhodamine 4-isomers still lacking. Herein, we present facile protecting-group-free 4-carboxyrhodamines’ based on nucleophilic...
ABSTRACT Here we report a small molecule probe for single localisation microscopy (SMLM) of tubulin in living and fixed cells. We explored series constructs composed taxanes spontaneously blinking far-red dye hydroxymethyl silicon-rhodamine (HMSiR). found that the linker length profoundly affects permeability off-targeting. The best performing probe, HMSiR-tubulin, is cabazitaxel 6’-regioisomer HMSiR bridged by C6 linker. Microtubule diameters ≤50 nm can be routinely measured SMLM...