A5019298056- Chromatin Remodeling and Cancer
- Epigenetics and DNA Methylation
- Neurogenesis and neuroplasticity mechanisms
- RNA Research and Splicing
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- Multiple Sclerosis Research Studies
- Stress Responses and Cortisol
- RNA modifications and cancer
- Hedgehog Signaling Pathway Studies
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Neuroendocrine regulation and behavior
- Health, Environment, Cognitive Aging
- Genetics and Neurodevelopmental Disorders
- Corporate Social Responsibility Reporting
- Neural dynamics and brain function
- Sustainable Supply Chain Management
- Economic Sanctions and International Relations
- Congenital heart defects research
- Cancer-related gene regulation
- Developmental Biology and Gene Regulation
- Autonomous Vehicle Technology and Safety
- Polyomavirus and related diseases
- Social Robot Interaction and HRI
Ruhr University Bochum
2021-2024
National Institute of Mental Health
2024
National Institute of Mental Health
2023-2024
Wellcome Centre for Integrative Neuroimaging
2023-2024
Lake Forest College
2023-2024
University of Oxford
2023-2024
Radboud University Nijmegen
2024
University of Toronto
2024
Mental Health Australia
2023
The University of Melbourne
2023
Neurogenesis is a key developmental event through which neurons are generated from neural stem/progenitor cells. Chromatin remodeling BAF (mSWI/SNF) complexes have been reported to play essential roles in the neurogenesis of central nervous system. However, whether required for neuron generation olfactory system unknown. Here, we identified onscBAF and ornBAF complexes, specifically present stem cells (oNSCs) receptor (ORNs), respectively. We demonstrated that BAF155 subunit highly expressed...
Abstract As the burden of neurodegenerative diseases increases, time-limited clinic encounters do not allow quantification complex neurological functions. Patient-collected digital biomarkers may remedy this, if they provide reliable information. However, psychometric properties tools remain largely un-assessed. We developed a smartphone adaptation cognitive test, Symbol-Digit Modalities Test (SDMT) by randomizing test’s symbol-number codes and testing sequences. The SDMT showed comparable...
In vivo neuroimaging studies have established several reproducible volumetric sex differences in the human brain, but causes of such are hard to parse. While mouse models useful for understanding cellular and mechanistic bases sex-specific brain development, there been no attempts formally compare neuroanatomical ascertain how well they translate. Addressing this question would shed critical light on use as a translational model provide insights into degree which volume conserved across...
During early cortical development, neural stem cells (NSCs) divide symmetrically to expand the progenitor pool, whereas, in later stages, NSCs asymmetrically self-renew and produce other cell types. The timely switch from such proliferative differentiative division critically determines neuron numbers. However, mechanisms that limit late development are not fully understood. Here, we show BAF (mSWI/SNF) complexes restrict competence promote neuronal differentiation corticogenesis....
The BAF chromatin remodeling complex plays an essential role in brain development. However its function postnatal neurogenesis hippocampus is still unknown. Here, we show that dentate gyrus (DG), the BAF170 subunit of expressed radial glial-like (RGL) progenitors and cell types involved subsequent steps adult including mature astrocytes. Conditional deletion during cortical late as well depletes pool RGL cells DG, promotes terminal astrocyte differentiation. These derangements are...
In vivo neuroimaging studies have established several reproducible volumetric sex differences in the human brain, but causes of such are hard to parse. While mouse models useful for understanding cellular and mechanistic bases sex-specific brain development, there been no attempts formally compare neuroanatomical ascertain how well they translate. Addressing this question would shed critical light on use as a translational model provide insights into degree which volume conserved across...
The abundance of basal progenitors (BPs), radial glia (bRGs) and intermediate (bIPs), in primate brain has been correlated to the high degree cortical folding. Here we examined role BAF155, a subunit chromatin remodeling BAF complex, generation progenitor heterogeneity. conditional deletion BAF155 led diminished bIP pool increased number bRGs, due delamination apical RGs. We found that is required for normal activity neurogenic transcription factor PAX6, thus controlling expression genes are...
The multi-subunit chromatin-remodeling SWI/SNF (known as BAF for Brg/Brm-associated factor) complexes play essential roles in development. Studies have shown that the loss of individual subunits often affects local chromatin structure and specific transcriptional programs. However, we do not fully understand how function development because no animal mutant had been engineered to lack entire complexes. Importantly, recently reported double conditional knock-out (dcKO) BAF155 BAF170 core mice...
Gene transfer of a human cocaine hydrolase (hCocH) derived from butyrylcholinesterase (BChE) by 5 mutations (A199S/F227A/S287G/A328W/Y332G) has shown promise in animal studies for treatment addiction. To predict the physiological fate and immunogenicity this enzyme humans, comparable was created tested conspecific host. Thus, similar (A199S/S227A/S287G/A328W/Y332G) were introduced into mouse BChE to obtain CocH (mCocH). The cDNA incorporated viral vectors based on: a) serotype-5...
Fine-tuned gene expression is crucial for neurodevelopment. The program tightly controlled at different levels, including RNA decay. N6-methyladenosine (m6A) methylation-mediated degradation of essential brain development. However, m6A methylation impacts not only stability, but also other metabolism processes. How decay contributes to development largely unknown. Here, we show that Exosc10, a exonuclease subunit the exosome complex, indispensable forebrain We report cortical cells undergo...
Our long-term goal is to employ smartphone-embedded sensors measure various neurological functions in a patient-autonomous manner. The interim develop simple smartphone tests (apps) and evaluate the clinical utility of these by selecting optimal outcomes that correlate well with clinician-measured disability different domains. In this paper, we used prospectively-acquired data from 112 multiple sclerosis (MS) patients 15 healthy volunteers (HV) assess performance optimize Level Test. test...
Increase in the size of human neocortex―acquired evolution―accounts for unique cognitive capacity humans. This expansion reflects evolutionarily enhanced proliferative ability basal progenitors (BPs), including radial glia and intermediate (bIPs) mammalian cortex, which may have been acquired through epigenetic alterations BPs. However, how epigenome BPs differs across species is not known. Here, we report that histone H3 acetylation a key regulation bIP amplification cortical expansion....
The conformational landscape of HIV-1 protease (PR) can be experimentally characterized by pulsed-EPR double electron-electron resonance (DEER). For this characterization, nitroxide spin labels are attached to an engineered cysteine residue in the flap region PR. DEER distance measurements from spin-labels contained within each homodimer provide a detailed description sampling apo-enzyme as well induced shifts function inhibitor binding. distribution profiles further interpreted terms...
Radial neuronal migration is a key neurodevelopmental event indispensable for proper cortical laminar organization. Cortical neurons mainly use glial fiber guides, cell adhesion dynamics, and cytoskeletal remodeling, among other discrete processes, to radially trek from their birthplace final layer positions. Dysregulated radial can engender mis-lamination, leading disorders. Epigenetic factors, including chromatin remodelers have emerged as formidable regulators of corticogenesis. Notably,...
Technological advances, lack of medical professionals, high cost face-to-face encounters, and disasters such as the COVID-19 pandemic fuel telemedicine revolution. Numerous smartphone apps have been developed to measure neurological functions. However, their psychometric properties are seldom determined. It is unclear which designs underlie eventual clinical utility tests. We Neurological Function Tests Suite (NeuFun-TS) systematically evaluating against gold standard complete examination...
We studied the concordance of transgene expression in transplanted heart using bicistronic adenoviral vector coding for a interest (human carcinoembryonic antigen: hCEA - beta human chorionic gonadotropin: betahCG) and marker imaging sodium iodide symporter: hNIS).Inbred Lewis rats were used syngeneic heterotopic cardiac transplantation. Donor rat hearts perfused ex vivo 30 min prior to transplantation with University Wisconsin (UW) solution (n=3), 10(9) pfu/ml adenovirus expressing hNIS...
Oligodendrocytes are responsible for axon myelination in the brain and spinal cord. Generation of oligodendrocytes entails highly regulated multistage neurodevelopmental events, including proliferation, differentiation maturation. The chromatin remodeling BAF (mSWI/SNF) complex is a notable regulator neural development. In our previous studies, we determined indispensability scaffolding subunits BAF155 BAF170 neurogenesis, whereas their role gliogenesis unknown. Here, show that expression...
This paper examines whether the ESG reporting transparency of listed firms in UK can play a role mitigating impact COVID-19 pandemic. We investigate 350 FTSE350 index from 2016 to 2021 with daily data on stock performance and annual financial performance. The empirical results show that high disclosure score have lower volatility during For these firms, negative relationship between performance, as well their main driving factors, is Among we identify lockdown announcement, quantitative...
Background: The mental health impacts of climate change-related disasters are significant. However, access to services is often limited by the availability trained clinicians. Although building local community capability for response prioritised in policy settings, lack evidence-based programs problematic. aim this study was test efficacy Skills Life Adjustment and Resilience programme (SOLAR) delivered members following compound (drought, wildfires, pandemic-related lockdowns) Australia.
Enrichment of basal progenitors (BPs) in the developing neocortex is a central driver cortical enlargement. The transcription factor Pax6 known as an essential regulator generation BPs. H3 lysine 9 acetylation (H3K9ac) has emerged crucial epigenetic mechanism that activates gene expression program required for BP pool amplification. In this current work, we applied immunohistochemistry, RNA sequencing, chromatin immunoprecipitation and yeast two-hybrid assay to reveal BP-genic effect...