An increase in plastic waste and its release into the environment has led to health concerns over microplastics (MPs) environment. The intestinal mucosal layer is a key defense mechanism against ingested MPs, preventing migration of particles other parts body. MP through mucus challenging study due difficulties obtaining intact layers for testing numerous formulations, shapes, sizes microplastics. Previous studies have primarily used from animals, hydrogel models, samples body as...

The mucus layer lining the gastrointestinal tract is essential for gut health, providing a protective barrier against pathogens while maintaining symbiosis with microbiome. Its disruption hallmark of diseases like ulcerative colitis. While glycan foraging by bacteria thought to initiate disruption, its impact on structural properties remains poorly understood, largely due lack physiologically relevant models. To address this gap, we developed method collect human-cell-derived that closely...

Mechanosensitive mechanisms are often used to sense damage tissue structure, stimulating matrix synthesis and repair. While this kind of mechanoregulatory process is well recognized in eukaryotic systems, it not known whether such a occurs bacteria. In Vibrio cholerae, antibiotic-induced the load-bearing cell wall promotes increased signaling by two-component system VxrAB, which stimulates synthesis. Here we show that changes mechanical stress within envelope sufficient stimulate VxrAB...

Conventional building materials, such as concrete, steel, and brick, are energy-intensive to produce, contribute significant carbon emissions, deplete finite natural resources. In response critical environmental issues caused by these non-renewable mycelium-based composites (MBCs) have attracted widespread attention. MBCs provide a renewable alternative where mycelium grows on lignocellulosic fibers forming lightweight, low energy cost, an effective insulating material. However, the...

Engineered living materials (ELMs) are an emerging class of that synthesized and/or populated by cells to achieve novel functionalities including self‐healing and sensing. Providing nutrients within ELM over prolonged periods remains a major technical challenge limits the service life ELMs. Bone maintains for decades delivering through network nanoscale channels punctuated microscale pores. Nutrient transfer in bone is enabled mechanical loading experienced material during regular use....

Abstract Multi-phenotypic screening of zebrafish larvae, such as monitoring the heart and tail activities, is important in biological assays. Microfluidic devices have been developed for phenotypic assays, but simultaneous lateral–dorsal same larva a single chip yet to be achieved. We present multi-phenotypic microfluidic device movement rate (HR) 5–7-day postfertilization larvae. Tail movements were stimulated using electric current quantified terms response duration (RD) beat frequency...

Microfluidic devices are being used for phenotypic screening of zebrafish larvae in fundamental and pre-clinical research. A challenge the broad use these microfluidic is their low throughput, especially behavioral assays. Previously, we introduced tail locomotion a semi-mobile larva evoked on-demand with electric signal device. Here, report lessons learned increasing number specimens from one to four this device.Multiple parameters including loading testing time per fish orientation...

Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play roles in purinergic signaling the nervous system. A link between Panx1 activity and neurodegenerative disorders including Parkinson's disease (PD) has been suggested, but experimental evidence is limited. Here, a zebrafish model of PD was produced by exposing panx1a+/+ panx1a-/- larvae to 6-hydroxydopamine (6-OHDA). Electrical stimulation microfluidic chip quantitative real-time-qPCR tested role both pathological normal...

Biologically produced materials are an attractive alternative to traditional such as metals and plastics offer improved functionalities better biodegradability biocompatibility. Polysaccharides example of a biologically that can have range chemical physical properties including high stiffness weight ratios thermal stability. Biomanufactured bacterial polysaccharides come with many advantages being non-toxic mechanically robust relative proteins lipids, which also secreted by bacteria...

Biologically produced materials are an attractive alternative to traditional such as metals and plastics offer improved functionalities better biodegradability biocompatibility. Polysaccharides example of biologically that can have a range chemical physical properties including high stiffness weight ratios thermal stability. synthesized by bacteria come with many advantages being non-toxic mechanically robust relative proteins lipids, which also secreted generate biofilm. Biomanufacturing...

Abstract In tissues with mechanical function, the regulation of remodeling and repair processes is often controlled by mechanosensitive mechanisms; damage to tissue structure detected changes in stress strain, stimulating matrix synthesis repair. While this mechanoregulatory feedback process well recognized animals plants, it not known whether such a occurs bacteria. Vibrio cholerae , antibiotic-induced load-bearing cell wall promotes increased signaling two-component system VxrAB, which...

The signaling molecular mechanisms in zebrafish response to electricity are unknown, so here we asked if changes dopaminergic pathways can affect their electrically-evoked locomotion. To answer this question, the effects of multiple selective and non-selective dopamine compounds on electric larvae is investigated. A microfluidic device with enhanced control experimentation used, which features a novel design immobilize four parallel expose them current that induces tail In 6 days...

Abstract Engineered living materials (ELMs) are an emerging class of that is synthesized and/or populated by cells. Maintaining cells within ELM over prolonged periods remains a major technical challenge limits the service life material. Biological regularly maintain robust populations Bone maintains for decades delivering nutrients through network nanoscale channels punctuated microscale pores. Nutrient transfer in bone enabled mechanical loading experienced during regular use. Here we...

Electrical stimulation of brain or muscle activities has gained attention for studying the molecular and cellular mechanisms involved in electric-induced responses. We recently showed zebrafish's response to electricity. Here, we hypothesized that this is affected by dopaminergic signaling pathways. The effects multiple dopamine agonists antagonists on electric 6 days-postfertilization zebrafish larvae were investigated using a microfluidic device with enhanced control experimentation...

Multi-phenotypic screening of multiple zebrafish larvae plays an important role in enhancing the quality and speed biological assays. Many microfluidic devices have been presented for phenotypic assays, but multi-organ larvae, from different needed orientations, a single device that can enable rapid large-sample testing is yet to be achieved. Here, we propose multi-phenotypic quadruple-fish chip simultaneous monitoring fin movement heart activity 5–7-day postfertilization trapped chip. In...

Abstract Multi-phenotypic screening of multiple zebrafish larvae plays an important role in enhancing the quality and speed biological assays. Many microfluidic platforms have been presented for phenotypic assays, but multi-organ larvae, from different needed orientations, a single device that can enable rapid large-sample testing is yet to be achieved. Here, we propose multi-phenotypic quadruple-fish chip simultaneous monitoring heart activity fin movement 5–7-day postfertilization trapped...

Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play essential roles in purinergic signaling the nervous system. Several studies suggest a link between Panx1-based activity and neurodegenerative disorders including Parkinson’s disease (PD), but experimental evidence is limited. Here, we applied behavioral molecular screening of zebrafish larvae to examine role Panx1 both pathological normal conditions, using electrical stimulation microfluidic chip RT-qPCR. A model PD was...

Microfluidic devices have been introduced for phenotypic screening of zebrafish larvae in both fundamental and pre-clinical research. One the remaining challenges broad use microfluidic is their limited throughput, especially behavioural assays. Previously, we tail locomotion a semi-mobile larva evoked on-demand with electric signal device. Here, report lessons learned increasing number specimens from one to four this Multiple parameters including loading testing time per fish orientation...

We previously showed that electric current can cause zebrafish larvae to move towards the anode pole along a microchannel. For semi-mobile larva, we observed response electricity depended on magnitude. The effects of signal direction, voltage magnitude and habituation repeated exposures pulses were not characterized. Here, this knowledge gap was addressed by exploiting these parameters in microfluidic device with head-trap immobilize larva downstream chamber for tail movement phenotypic...