A5043483286- Bladder and Urothelial Cancer Treatments
- Urinary and Genital Oncology Studies
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Urological Disorders and Treatments
- Renal Transplantation Outcomes and Treatments
- Renal cell carcinoma treatment
- MicroRNA in disease regulation
- Inflammatory Biomarkers in Disease Prognosis
- Cytomegalovirus and herpesvirus research
- Organ Transplantation Techniques and Outcomes
- Immunotherapy and Immune Responses
- Prostate Cancer Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Urinary Bladder and Prostate Research
- RNA Interference and Gene Delivery
- Cancer, Lipids, and Metabolism
- MRI in cancer diagnosis
- Cyclopropane Reaction Mechanisms
- Reproductive System and Pregnancy
- Neuroinflammation and Neurodegeneration Mechanisms
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
Osaka University of Pharmaceutical Sciences
2023-2024
Osaka Medical and Pharmaceutical University
2013-2023
Harvard University
2016-2021
Brigham and Women's Hospital
2016-2021
Nozaki Tokushukai Hospital
2020
Fukuoka Tokushukai Hospital
2020
Gifu University
2016-2019
Osaka Minami Medical Center
2019
Mayo Clinic in Arizona
2018
Osaka University Hospital
2015
Older organs represent an untapped potential to close the gap between demand and supply in organ transplantation but are associated with age-specific responses injury increased immunogenicity, thereby aggravating transplant outcomes. Here we show that cell-free mitochondrial DNA (cf-mt-DNA) released by senescent cells accumulates aging augments immunogenicity. Ischemia reperfusion induces a systemic increase of cf-mt-DNA promotes dendritic cell-mediated, inflammatory responses. Comparable...
Adipose-derived stromal cell (ASC), known as one of the mesenchymal stem cells (MSCs), is a promising tool for regenerative medicine; however, effect ASCs on tumor growth has not been studied sufficiently. We investigated hypothesis that have an inhibitory metastatic progression. To evaluate in vitro prostate cancer (PCa), direct coculture and indirect separate culture experiments with PC3M-luc2 human were performed, administered to cell-derived tumor-bearing nude mice vivo experiment. also...
The Warburg effect is a well-known feature in cancer-specific metabolism. We previously reported on the role of microRNA (miR)-145 as tumor-suppressor human bladder cancer (BC) cells. In this study, we reveal that miR-145 decreases by silencing KLF4 BC expression levels were significantly lower clinical samples and cell lines compared to those normal tissues HUC Luciferase assay results showed directly bound 3'UTR KLF4, which was shown be overexpressed using Western blot analysis...
Objectives To assess whether bipolar transurethral resection of the prostate ( B ‐ TURP ) using TUR is ® system has a similar level efficacy and safety to that traditional monopolar M ), evaluate impact on postoperative urethral stricture rates over 36‐month follow‐up period. Patients Methods A total 136 patients with benign prostatic obstruction were randomised undergo either or conventional , regularly followed for 36 months after surgery. The primary endpoint was safety, which included...
Bladder cancer is one of the most difficult malignancies to control. We explored use a novel RNA-interference method for driver oncogene regulating specific energy metabolism by combination treatment with small interfering RNA (siRNA) and microRNA. After transfection T24 253JB-V cells miR-145 and/or siR-PTBP1, we examined effects cell growth gene expression performing trypan blue dye exclusion test, Western blot, Hoechst 33342 staining, reverse transcription polymerase chain reaction...
Introduction and Objectives. MicroRNA (miRNA) expression is altered in urologic malignancies, including urothelial carcinoma of the bladder (UCB). Individual miRNAs have been shown to modulate multiple signaling pathways that contribute BC. To identify a panel miRNA signature can predict aggressive phenotype from normal nonaggressive counterpart using levels assess prognostic value this specific markers patients with UCB. Methods . determine candidate as biomarkers for dividing type UCB, was...
Sex-specific influences have been shown for a variety of diseases. Whether donor or recipient sex and hormone levels impact alloimmune responses remains unclear. In unifactorial multifactorial analyses more than 400 000 SRTR listed kidney transplant patients, we found that younger female recipients had an inferior death-censored graft survival was independent sex. contrast, superior in older recipients, suggesting the hormones over chromosomal mismatches. Those clinical changes were...
Inflammatory responses associated with ischemia/reperfusion injury (IRI) play a central role in alloimmunity and transplant outcomes. A key event driving these inflammatory is the burst of reactive oxygen species (ROS), hydrogen peroxide (H2 O2 ) as most abundant form that occurs result surgical implantation donor organ. Here, we used syngeneic rat renal IRI model to evaluate therapeutic properties APP-103, polyoxalate-based copolymer molecule containing vanillyl alcohol (VA) exhibits high...
Abstract Age impacts alloimmunity. Effects of aging on T‐cell metabolism and the potential to interfere with immunosuppressants have not been explored yet. Here, we dissected metabolic pathways CD4 + CD8 T cells in offer novel immunosuppressive targets. Upon activation, from old mice failed exhibit adequate reprogramming resulting into compromised pathways, including oxidative phosphorylation (OXPHOS) glycolysis. Comparable results were also observed elderly human patients. Although...
We previously reported that the level of microRNA (miR)-145 is attenuated in human bladder cancer cells. In this current study, we investigated whether intravesical administration miR-145 could be a potential therapeutic strategy for controlling by using an orthotopic xenograft model. Following transfection 253J B-V cells with miR-145, effects ectopic expression were examined performing MTT, Western blotting analysis, Hoechst33342 staining, and wound healing assay vitro. Also, mouse model...
The combination of platelet count and neutrophil to lymphocyte ratio (COP-NLR) has been shown provide prognostic information in several cancers, whereas its value renal cell carcinoma (RCC) not reported. objective the present study was examine preoperative COP-NLR patients with localized RCC undergoing nephrectomy.The record 268 patients, who underwent nephrectomy due a diagnosis at our institute analyzed study. cut-off NLR were defined by receive operating characteristic (ROC) analysis...
BackgroundGiven their unique capacity for antigen uptake, processing, and presentation, antigen-presenting cells (APCs) are critical initiating regulating innate adaptive immune responses. We have previously shown the role of nicotinamide adenine dinucleotide (NAD+) in T-cell differentiation independently cytokine milieu, whereas precise mechanisms remained unknown.ObjectiveThe objective this study is to further dissect mechanism actions NAD+ determine effect APCs on NAD+-mediated...
Abstract It has been well established that micro RNA (miR)‐143 is downregulated in human bladder cancer ( BC ). Recent precision medicine shown mutations are frequently observed FGFR 3, RAS and PIK 3 CA genes, all of which correlate with signaling networks. We have previously miR‐143 suppresses cell growth by inhibiting networks several cancers including . In the present study, we showed synthetic negatively regulated ‐binding protein Musashi‐2 MSI 2) lines. 2 an regulates stability certain...
Immunosuppression in elderly recipients has been underappreciated clinical trials. Here, we assessed age-specific effects of the calcineurin inhibitor tacrolimus (TAC) a murine transplant model and its relevance on human T cells. Old recipient mice exhibited prolonged skin graft survival compared with young animals after TAC administration. More important, half dose was sufficient old to achieve comparable systemic trough levels. administration able reduce proinflammatory interferon-γ...
The combination of C-reactive protein and albumin, the Glasgow Prognostic Score (GPS), had independent prognostic value in patients with varying cancers, except for upper tract urothelial carcinoma (UTUC). aim this study was to describe relationship between GPS survival UTUC after adjustment other factors.We queried 2 databases. Retrospective clinical series on localized managed by nephroureterectomy bladder cuff, whom data from Yamaguchi Uro-Oncology Group Osaka Medical College registry,...
Boron neutron capture therapy (BNCT) is a selective radiation treatment for tumors that preferentially accumulate drugs carrying the stable boron isotope, 10B. BNCT has been evaluated clinically as an alternative to conventional of brain tumors, and more recently, recurrent advanced head neck cancer. Here we investigated effect on prostate cancer (PCa) using in vivo mouse xenograft model have developed.Mice bearing xenotransplanted androgen-independent human PCa cell line, PC3, were divided...
Background: There is emerging evidence that radiographic progression-free survival (rPFS) highly correlated with overall (OS), potentially serving as an indicator of treatment outcome for castration-resistant prostate cancer (CRPC). The objective this study to assess rPFS and specific antigen (PSA) response in sequential using androgen signaling inhibitors (ASIs) including abiraterone enzalutamide newly diagnosed CRPC. Methods: Propensity score matching was performed reduce bias by...