A5064744050- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Lymphatic System and Diseases
- Hedgehog Signaling Pathway Studies
- Zebrafish Biomedical Research Applications
- Wound Healing and Treatments
- Cardiac Fibrosis and Remodeling
- Liver physiology and pathology
- Autophagy in Disease and Therapy
- Cancer Cells and Metastasis
- Organ Transplantation Techniques and Outcomes
- Cancer, Lipids, and Metabolism
- Pluripotent Stem Cells Research
- Cancer-related Molecular Pathways
- Cardiac Ischemia and Reperfusion
- Cerebrovascular and Carotid Artery Diseases
- Neurogenesis and neuroplasticity mechanisms
- Liver Disease Diagnosis and Treatment
- Chemokine receptors and signaling
- Spinal Cord Injury Research
- Peroxisome Proliferator-Activated Receptors
Osaka Medical and Pharmaceutical University
2012-2023
Wake Forest University
2019
Forest Institute
2019
Charlottesville Medical Research
2019
John Wiley & Sons (United States)
2019
Foundation for Biomedical Research and Innovation
2005-2012
Tokyo Women's Medical University
2009-2012
Northwestern University
2008-2012
Medizinische Hochschule Hannover
2012
Northwestern Memorial Hospital
2012
In the inflamed cornea, there is a parallel outgrowth of blood and lymphatic vessels into normally avascular cornea. We tested whether adaptive and/or innate immune cells were actively involved in genesis new vessels. Our results indicate that (CD11b+ macrophages, but not CD11c+ dendritic cells) physically contributed to lymphangiogenesis under pathological conditions bone marrow–derived CD11b+ macrophages expressed endothelial markers such as LYVE-1 Prox-1 corneal stromata mice....
Axonal guidance and vascular patterning share several cues, including proteins in the netrin family. We demonstrate that netrins stimulate proliferation, migration, tube formation of human endothelial cells vitro this stimulation is independent known receptors. Suppression netrin1a messenger RNA zebrafish inhibits sprouting, implying a proangiogenic role for during vertebrate development. also show accelerate neovascularization an vivo model ischemia they reverse neuropathy vasculopathy...
Noncellular differentiation effects have emerged as important mechanisms mediating therapeutic of stem or progenitor cell transplantation. Here, we investigated the expression patterns and sources humoral factors their regional systemic biological after bone marrow (BM)-derived endothelial (EPC) transplantation into ischemic myocardium. Although most transplanted EPCs disappeared within a week, up-regulation multiple was sustained for longer than two weeks, which correlated well with...
Background— The function of bone marrow–derived endothelial progenitor cells (EPCs) in repair ischemic tissue has been the subject intense scrutiny, and capacity these to contribute significantly new blood vessels remains controversial. possibility that EPCs could act as reservoirs cytokines implied by several observations; however, a specific role for cytokine delivery not identified. Methods Results— We performed series experiments revealed rapid recruitment myocardium very short periods...
Background— Sonic hedgehog (Shh) is a prototypical morphogen known to regulate epithelial-mesenchymal interaction during embryonic development. Recent observations indicate that exogenous administration of Shh can induce angiogenesis and may accelerate repair ischemic myocardium skeletal muscle. Because plays pivotal role in wound repair, we hypothesized activation the pathway promote favorable effect on microvascular remodeling cutaneous healing thereby closure. diabetes associated with...
Notch receptors are important mediators of cell fate during embryogenesis, but their role in adult physiology, particularly postnatal angiogenesis, remains unknown. Of the receptors, only Notch1 and Notch4 expressed vascular endothelial cells. Here we show that blood flow recovery neovascularization response to hindlimb ischemia haploinsufficient global or endothelial-specific Notch1(+/-) mice, not Notch4(-/-) were impaired compared with wild-type mice. The expression growth factor (VEGF)...
Delayed reendothelialization contributes to restenosis after angioplasty and stenting in diabetes. Prior data have shown that bone marrow (BM)-derived endothelial progenitor cells (EPCs) contribute recovery arterial injury. We investigated the hypothesis EPC contribution may be impaired diabetes, resulting delayed reendothelialization. Reendothelialization was significantly reduced diabetic mice compared with nondiabetic a wire-induced carotid denudation model. The neoendothelium Tie2/LacZ...
Background— Estradiol (E 2 ) modulates the kinetics of circulating endothelial progenitor cells (EPCs) and favorably affects neovascularization after ischemic injury. However, roles estrogen receptors α (ERα) β (ERβ) in EPC biology are largely unknown. Methods Results— In response to E , migration, tube formation, adhesion, estrogen-responsive element–dependent gene transcription activities were severely impaired EPCs obtained from ERα-knockout mice (ERαKO) moderately ERβKO EPCs. The number...
Background Endothelial progenitor cells (EPCs) were shown to have angiogenic potential contributing neovascularization. However, a clear definition of mouse EPCs by cell surface markers still remains elusive. We hypothesized that CD34 could be used for identification and isolation functional from bone marrow. Methodology/Principal Findings CD34+ cells, c-Kit+/Sca-1+/Lin− (KSL) c-Kit+/Lin− (KL) Sca-1+/Lin− (SL) isolated marrow mononuclear (BMMNCs) using fluorescent activated sorting. EPC...
Adipose-derived stromal cell (ASC), known as one of the mesenchymal stem cells (MSCs), is a promising tool for regenerative medicine; however, effect ASCs on tumor growth has not been studied sufficiently. We investigated hypothesis that have an inhibitory metastatic progression. To evaluate in vitro prostate cancer (PCa), direct coculture and indirect separate culture experiments with PC3M-luc2 human were performed, administered to cell-derived tumor-bearing nude mice vivo experiment. also...
Background— Endothelial progenitor cells (EPCs) are known to promote neovascularization in ischemic diseases. Recent evidence suggested that diabetic neuropathy is causally related impaired angiogenesis and deficient growth factors. Accordingly, we investigated whether could be reversed by local transplantation of EPCs. Methods Results— We found motor sensory nerve conduction velocities, blood flow, capillary density were reduced sciatic nerves streptozotocin-induced mice but recovered...
The primary structure of the macrophage lectin specific for galactose and N-acetylgalactosamine (macrophage asialoglycoprotein-binding protein, M-ASGP-BP) has been deduced from its cDNA sequence. M-ASGP-BP encoded a protein consisting 306 amino acid residues with molecular mass 34,242 daltons. sequence was highly homologous that rat liver asialoglycoprotein receptor (rat hepatic lectin, RHL), particularly RHL-1 (the major form throughout whole length, especially so in putative...
Adipose tissue is one of the promising sources multipotent stem cells in human. Human adipose-derived (hMADS) have recently been isolated and showed differentiation potential into multiple mesenchymal lineages vitro vivo. On basis these evidences, we examined therapeutic efficacy hMADS for fracture healing an immunodeficient rat femur non-union model. Local transplantation radiographically histologically promoted with significant improvement biomechanical function at sites compared local...
Background— CXC-chemokine receptor 4 (CXCR4) regulates the retention of stem/progenitor cells in bone marrow (BM), and CXCR4 antagonist AMD3100 improves recovery from coronary ligation injury by mobilizing BM to peripheral blood. Thus, we investigated whether also ischemia/reperfusion injury, which more closely mimics myocardial infarction patients, because blood flow is only temporarily obstructed. Methods Results— Mice were treated with single subcutaneous injections (5 mg/kg) or saline...
Abstract Several reports have shown the therapeutic effect of statins on bone formation and neovascularization. However, systemic administration is limited due to its metabolism in liver clearance digestive system. In addition, high-dose may cause adverse side effects. To avoid low-efficacy/frequent effects statin treatment, we utilized biodegradable gelatin hydrogel as a drug delivery system for fracture healing. A femoral was created rats with periosteum cauterization leading nonunion at 8...
CXC chemokine receptor 4 (CXCR4) is a specific for stromal-derived-factor 1 (SDF-1). SDF-1/CXCR4 interaction reported to play an important role in vascular development. On the other hand, therapeutic potential of endothelial progenitor cells (EPCs) fracture healing has been demonstrated with mechanistic insight vasculogenesis/angiogenesis and osteogenesis enhancement at sites fracture. The purpose this study was investigate influence pathway Tie2-lineage (including EPCs) bone formation. We...
Neovascularization is impaired in diabetes mellitus, which leads to the development of peripheral arterial disease and mainly attributed dysfunction endothelial progenitor cells (EPCs). Previous studies proved promotional effect curcumin on neovascularization wound healing diabetes. Thus, we hypothesize that could promote at sites hindlimb ischemia might take via modulating function EPCs. Streptozotocin-induced type 1 diabetic mice nondiabetic both received unilateral ischemic surgery....
Adipose-derived stem cells (AdSCs) have recently been considered a useful treatment tool for autoimmune disease because of their anti-inflammatory and immunosuppressive effects. We investigated the therapeutic effect intravenous AdSC transplantation in mouse model bleomycin-induced lung injury. AdSCs accumulated pulmonary interstitium inhibited both inflammation fibrosis lung, markedly improving survival rate mice with injury cell number-dependent manner. production pro-inflammatory...
We performed a series of investigations to test the hypothesis that combining angiogenic gene therapy and cytokine (CK)-induced endothelial progenitor cell mobilization would be superior either strategy alone for treatment chronic myocardial ischemia.A swine model ischemia murine acute infarction were used in this study. In both models, animals randomly assigned 1 4 groups: Combo group, intramyocardial vascular growth factor (VEGF)-2 transfer plus subcutaneous injection CKs; VEGF-2, VEGF-2...
The cell surface receptor α4 integrin plays a critical role in the homing, engraftment, and maintenance of hematopoietic progenitor cells (HPCs) bone marrow (BM). Down-regulation or functional blockade its ligand vascular adhesion molecule-1 mobilizes long-term HPCs. We investigated mobilization homing BM endothelial (EPCs). EPCs with colony-forming activity are exclusively integrin–expressing cells. In vivo, single dose anti–α4 antibody resulted increased circulating EPC counts for 3 d....