A5067236710- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Complement system in diseases
- Proteoglycans and glycosaminoglycans research
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
- Infant Nutrition and Health
- T-cell and B-cell Immunology
- Enzyme Structure and Function
- Invertebrate Immune Response Mechanisms
- Metabolism and Genetic Disorders
- Biochemical and Molecular Research
- Sphingolipid Metabolism and Signaling
- Cell Adhesion Molecules Research
- Virus-based gene therapy research
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Animal Genetics and Reproduction
- Chemokine receptors and signaling
- Lysosomal Storage Disorders Research
Ritsumeikan University
2012-2022
National Institute of Biomedical Innovation, Health and Nutrition
2022
Chubu University
2016
Agrobiotechnology Institute
2008-2009
Kyoto University
1998-2007
Kyoto Pharmaceutical University
1994-2007
Japan Science and Technology Agency
1999-2002
Kyushu University
1979-2000
RIKEN
1997
Tokyo Metropolitan Institute of Medical Science
1993-1994
Serum mannan-binding protein (MBP), a lectin specific for mannose and N-acetylglucosamine, was revealed to activate the complement system as measured by passive hemolysis using sheep erythrocytes coated with yeast mannan. In contrast, rat liver MBP, which shares many properties in common serum could not at all. The activation MBP inhibited effectively presence of haptenic sugars dependent absolutely upon C4, indicating that is initiated sugar binding activity proceeds through classical...
N-Glycolylneuraminic acid (NeuGc) is abundantly expressed in most mammals, but it not detectable humans. The expression of NeuGc controlled by cytidine monophospho-N-acetylneuraminic (CMP-NeuAc) hydroxylase activity. We previously cloned a cDNA for mouse CMP-NeuAc and found that the human genome contains homologue. report here molecular basis absence from HeLa cell library. encodes 486-amino protein, its deduced amino sequence lacks domain corresponding to N-terminal 104 acids although...
Rat and human al-acid glycoproteins contain 5 6 acidic asparagine-linked sugar chains in 1 molecule, respectively.The structures of these were studied by sequential exoglycosidase digestion combination with methylation analysis after being released from the polypeptide back bone hydrazinolysis.All oligosaccharides obtained both N-acetylneuraminic acid.Sialidase rat sample three biantennary complex type triantennary chains, outer which are composed GalPl+ 3GlcNAc and/or Galjll+ 4GlcNAc.In...
The state of aggregation found in water-soluble preparations an hepatic membrane protein responsible for the clearance serum asialoglycoproteins has been shown to result from self-associating properties a single oligomeric protein. smallest functional unit identifiable aqueous solution possessed estimated molecular weight 500,000 with each successive components increasing size by equal amount form series bearing integral ratio 1:2:3:4:5. tendency towards self-association was promptly and...
Journal Article Isolation and Characterization of a Mannan-Binding Protein from Human Serum Get access Nobuko KAWASAKI, KAWASAKI *College Medical Technology, Kyoto UniversitySakyo-ku, Kyoto, 606 Search for other works by this author on: Oxford Academic PubMed Google Scholar Toshisuke **Department Biological Chemistry, Faculty Pharmaceutical Sciences, Ikuo YAMASHINA The Biochemistry, Volume 94, Issue 3, July 1983, Pages 937–947, https://doi.org/10.1093/oxfordjournals.jbchem.a134437 Published:...
An hepatic receptor which recognizes and binds specifically to serum glycoproteins bearing terminal, nonreducing N-acetylglucosamine residues has been purified homogeneity by affinity chromatography from chicken liver. The isolated binding protein characterized as a water-soluble glycoprotein in sialic acid, galactose, mannose, glucosamine comprise 8% of the total molecule. reaction is saturable process proportional concentration. Evidence adduced indicate presence single high site with...
Antiserum raised against horseradish peroxidase (HRP) recognizes a neural specific carbohydrate antigen in Drosophila and other insects. The epitopic activity of the moiety HRP recognized by anti-HRP antiserum was measured newly developed enzyme-linked immunosorbent assay, which glycopeptides conjugated with bovine serum albumin were coated onto wells then reacted goat antiserum. sugar moieties released almond glycopeptidase A digestion pepsin digests subjected to pyridylamination....
Sialic acid (Sia) is a family of acidic nine-carbon sugars that occupies the nonreducing terminus glycan chains. Diversity Sia achieved by variation in linkage to underlying sugar and modification molecule. Here we identified Sia-dependent epitope specificity for GL7, rat monoclonal antibody, probe germinal centers upon T cell-dependent immunity. GL7 recognizes sialylated glycan(s), alpha2,6-linked N-acetylneuraminic (Neu5Ac) on lactosamine chain(s), both modification- linkage-dependent...
Serum mannan-binding protein (S-MBP), a lectin specific for mannose and N-acetylglucosamine, was documented to activate complement through the classical pathway. In this study, we examined mechanism that initiates activation. By passive hemolysis test using sheep erythrocytes coated with yeast mannan, activation of by human S-MBP shown proceed in absence C1q. The following binding studies 125I-labeled C1r2s2 C1s indicated activated form bound located on surface cells high affinity....
We isolated a cDNA encoding novel glucuronyltransferase from human placenta with the use of degenerate reverse transcriptase-polymerase chain reaction method. Degenerate primers were designed based upon amino acid sequence alignment rat (GlcAT-P) involved in biosynthesis carbohydrate epitope HNK-1 putative proteins Caenorhabditis elegans and Schistosoma mansoni. The new revealed an open reading frame coding for protein 335 acids type II transmembrane topology. displayed 43% identity to...
Cytidine monophospho-N-acetylneuraminic acid (CMP-NeuAc) hydroxylase, which is the key enzyme for synthesis of N-glycolylneuraminic (NeuGc), has been purified from cytosolic fraction mouse liver, as described in our previous paper. The amino sequences CMP-NeuAc and peptides obtained by lysylendopeptidase digestion, were used to synthesize specific oligonucleotide primers. A cDNA clone was a combination polymerase chain reaction rapid amplification ends. sequence contained an open reading...
Serum mannan-binding protein (MBP), which is a lectin specific for man nose and N-acetylglucosamine known to activate complement via the classical pathway, has been revealed have complement-dependent bactericidal activity, as tested on rough strains of Escherichia coli, K-12 B. The bacteria, had sensitized with purified human serum MBP in presence Ca2+, followed by incubation guinea pig complement, showed marked decrease colony forming ability compared those not lectin. effect depended...
The HNK-1 carbohydrate epitope, a sulfated glucuronic acid at the non-reducing terminus of glycans, is expressed characteristically on series cell adhesion molecules and synthesized through key enzyme, glucuronyltransferase (GlcAT-P). We generated mice with targeted deletion GlcAT-P gene. −/− exhibited normal development gross anatomical features, but adult mutant reduced long term potentiation Schaffer collateral-CA1 synapses defect in spatial memory formation. This first evidence that loss...
Abstract We have examined the region‐specific expression of mRNAs for four members rat FGF receptor family, FGFR‐1, FGFR‐2, FGFR‐3, and FGFR‐4, in brain by situ hybridization. The FGFR‐3 were expressed widely but differentially brain. However, FGFR‐4 mRNA was not FGFR‐1 strongly several regions including hippocampus, cerebellum, pedunculoptine tegmental nucleus. FGFR‐2 high choroid plexus, moderate fiber‐rich (the corpus callosum, external capsule, internal capsule) olfactory bulb. diffusely...
AbstractISP-1 is a new type of immunosuppressant, the structure which homologous to that sphingosine. In previous study, ISP-1 was found inhibit mammalian serine palmitoyltransferase, primary enzyme involved in sphingolipid biosynthesis, and reduce intracellular pool sphingolipids. induces apoptosis cytotoxic T cells, triggered by decreases levels this inhibition yeast (Saccharomyces cerevisiae) proliferation observed. This ISP-1-induced growth also addition, DNA duplication without...
The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules and also some glycolipids in the nervous system over wide range species from insect to mammal. involved cell–cell and/or cell–substrate interaction recognition during development system. In this study, we isolated novel glucuronyltransferase rat brain, which key enzyme biosynthesis glycoproteins. Based partial amino acid sequences, cDNA encoding glucuronyltransferase. primary structure...
A binding protein from liver which binds reversibly to yeast mannan depending on the presence or absence of calcium has been purified near homogeneity by affinity chromatography. The isolated 125I-mannan is proportional concentration and apparently an unsaturable process. Scatchard plot a curvilinear, indicating high site with dissociation constant 1.62 X 10(-8) M. Evidence presented show that recognizes mannose N-acetylglucosamine residues almost indiscriminately at same site. Physical...
The primary structure of the macrophage lectin specific for galactose and N-acetylgalactosamine (macrophage asialoglycoprotein-binding protein, M-ASGP-BP) has been deduced from its cDNA sequence. M-ASGP-BP encoded a protein consisting 306 amino acid residues with molecular mass 34,242 daltons. sequence was highly homologous that rat liver asialoglycoprotein receptor (rat hepatic lectin, RHL), particularly RHL-1 (the major form throughout whole length, especially so in putative...
Two variant glycopeptide constituents of an hepatic membrane protein responsible for the clearance circulating asialoglycoproteins have been isolated and characterized. Their relative abundance has estimated to be 14 2 mol, respectively, per mol intact protein. The larger component, I, is comprised sialic acid, galactose, mannose, glucosamine, aspartic acid in a molar ratio 3:3:2:5:1. smaller neutral fraction contains only two sugars relationship 8:2:1. Based on results sequential digestion...
Glycans are known as the third major class of biopolymers, next to DNA and proteins. They cover surfaces many cells, serving 'face' whereby other biomolecules viruses interact. The structure glycans, however, differs greatly from proteins in that they branched, opposed linear sequences amino acids or nucleotides. Therefore, storage glycan information databases, let alone their curation, has been a difficult problem. This caused duplicated efforts when integration is attempted between...