A5103077510- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Immune Cell Function and Interaction
- Proteoglycans and glycosaminoglycans research
- Neuroscience and Neuropharmacology Research
- Monoclonal and Polyclonal Antibodies Research
- Chemokine receptors and signaling
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Cellular transport and secretion
- Cytokine Signaling Pathways and Interactions
- Signaling Pathways in Disease
- Lipid Membrane Structure and Behavior
- Complement system in diseases
- Axon Guidance and Neuronal Signaling
- Neuropeptides and Animal Physiology
- Melanoma and MAPK Pathways
- Protein Kinase Regulation and GTPase Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolism and Genetic Disorders
- Pharmacological Effects of Natural Compounds
- Sphingolipid Metabolism and Signaling
- Amino Acid Enzymes and Metabolism
- Ubiquitin and proteasome pathways
- Protein Tyrosine Phosphatases
Kyoto University
2014-2024
Joseph Ayo Babalola University
2019
Institute of Molecular Biology and Biophysics
2014
Nihon University
2008-2009
Japan Science and Technology Agency
1999-2008
Kyoto Pharmaceutical University
1987-2007
National Institute for Basic Biology
1998
National Institutes of Natural Sciences
1998
RIKEN
1997
Case Western Reserve University
1995
Inflammatory factors such as tumor necrosis factor (TNF), interleukin 1 (IL-1), and lipopolysaccharide (LPS) greatly enhance the expression of group II phospholipase A2 (PLA2-II) mRNA, leading to increased secretion PLA2-II enzyme from rat-cultured astrocytes. The potent antiinflammatory agent dexamethasone suppressed induced by LPS. In vivo studies also demonstrated that level mRNA in brain with intravenous injection These results suggest plays important roles inflammatory response. Agents...
Phospholipase A2 (PLA2), EC 3.1.1.4, which catalyzes the release of free fatty acids from sn-2 position glycerophospholipids, has been extensively studied viewpoint eicosanoid production (Arita, H., Nakano, T., and Hanasaki, K. (1989) Prog. Lipid Res. 28, 273-301). Several lines evidence suggest that extracellular PLA2 is pathophysiologically related to some disorders, including inflammation hypersensitivity. Despite this, little known precise mechanism pathological processes as well their...
We isolated a cDNA encoding novel glucuronyltransferase from human placenta with the use of degenerate reverse transcriptase-polymerase chain reaction method. Degenerate primers were designed based upon amino acid sequence alignment rat (GlcAT-P) involved in biosynthesis carbohydrate epitope HNK-1 putative proteins Caenorhabditis elegans and Schistosoma mansoni. The new revealed an open reading frame coding for protein 335 acids type II transmembrane topology. displayed 43% identity to...
The HNK-1 carbohydrate epitope, a sulfated glucuronic acid at the non-reducing terminus of glycans, is expressed characteristically on series cell adhesion molecules and synthesized through key enzyme, glucuronyltransferase (GlcAT-P). We generated mice with targeted deletion GlcAT-P gene. −/− exhibited normal development gross anatomical features, but adult mutant reduced long term potentiation Schaffer collateral-CA1 synapses defect in spatial memory formation. This first evidence that loss...
The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules and also some glycolipids in the nervous system over wide range species from insect to mammal. involved cell–cell and/or cell–substrate interaction recognition during development system. In this study, we isolated novel glucuronyltransferase rat brain, which key enzyme biosynthesis glycoproteins. Based partial amino acid sequences, cDNA encoding glucuronyltransferase. primary structure...
Mannan-binding protein (MBP), a Ca2+-dependent mammalian lectin specific for mannose and N-acetylglucosamine, is an important serum component associated with innate immunity. MBP activates complement functions as direct opsonin on binding to mannooligosaccharide-bearing pathogens. We have found that recognizes binds specifically oligosaccharide ligands expressed the surfaces of human colorectal carcinoma. Interestingly, recombinant vaccinia virus carrying gene was demonstrated possess potent...
The HNK-1 carbohydrate epitope is expressed on several neural adhesion glycoproteins and as a glycolipid, involved in cell interactions. structural element of the common to glycolipids has been determined be sulfate-3-GlcAβ1→ 3Galβ1→4GlcNAc. glucuronyltransferase sulfotransferase are considered key enzymes biosynthesis this because rest structure occurs often glycoconjugates. Here we describe isolation rat cDNA via an expression cloning strategy. clone finally isolated predicts protein 356...
Recent studies have shown that plasma levels of the biologically inactive prohormone for brain natriuretic peptide (proBNP) are increased in patients with heart failure. This can contribute to a reduction effectiveness circulating BNP and exacerbate failure progression. The precise mechanisms governing increase proBNP remain unclear, however.
We have cloned a novel protein kinase from human cerebellum and named it LZK (leucine zipper-bearing kinase). The cDNA encoded 966-amino acid polypeptide that contains catalytic domain double leucine/isoleucine zippers separated by short spacer region. amino sequence of the was hybrid between those in serine/threonine tyrosine kinases, indicating belongs to subfamily mixed lineage (MLK) family. most similar DLK (Holtzman, L. B., Merritt, S.E., Fan, G. (1994) J. Biol. Chem. 269, 30808-30817),...
Immunogenicity is a major caveat of protein therapeutics. In particular, the long-term administration therapeutic agents leads to generation antidrug antibodies (ADAs), which reduce drug efficacy while eliciting adverse events. One promising solution this issue use mirror-image proteins consisting d-amino acids, are resistant proteolytic degradation in immune cells. We have recently reported chemical synthesis enantiomeric form variable domain antibody heavy chain (d-VHH). However,...
Serum mannan binding protein (MBP), a mannose/N-acetylglusosamine-specific lectin, is important in innate immunity. In order to elucidate the mechanism underlying wide intra- and interracial variety MBP serum level, we have studied transcriptional regulation of human MBP. Rapid amplification cDNA ends (5' RACE) analysis Hep G2 RNA indicated presence novel exon, designated as "exon 0," upstream previously identified exon 1 [Taylor, M.E. et al. (1989) Biochem. J. 262, 763-771]. Two mRNAs with...
Recently, embryonic chicken brain extract was shown to contain a glucuronyltransferase, which transfers glucuronic acid from UDP-glucuronic glycolipid acceptors (neolactotetraosyl ceramide). The enzyme also suggested transfer glycoprotein (asialoorosomucoid) (Das, K. K., Basu, M., S., Chou, D. H., and Jungalwala, F. B. (1991) J. Biol. Chem. 266, 5238-5243). In this study, the glucuronyltransferase activity in rat separated into two groups by acid-Sepharose CL-6B column chromatography....
The HNK-1 carbohydrate epitope is found on many neural cell adhesion molecules. Its structure characterized by a terminal sulfated glucuronyl acid. glucuronyltransferases, GlcAT-P and GlcAT-S, are involved in the biosynthesis of epitope, as major enzyme. We overexpressed purified recombinant human from Escherichia coli. Analysis its enzymatic activity showed that it catalyzed transfer reaction for N-acetyllactosamine (Galβ1-4GlcNAc) but not lacto-N-biose (Galβ1-3GlcNAc) an acceptor...
HNK-1 (human natural killer-1) glyco-epitope, a sulfated glucuronic acid attached to N-acetyllactosamine on the nonreducing termini of glycans, is highly expressed in nervous system. Our previous report showed that mice lacking glucuronyltransferase (GlcAT-P), key enzyme for biosynthesis epitope, reduced long term potentiation at hippocampal CA1 synapses. In this study, we identified an α-amino-3-hydroxy-5-methylisoxazole propionate (AMPA)-type glutamate receptor subunit, GluR2, which...
The glycosylation of glycoproteins and glycolipids is important for central nervous system development function. Although the roles several carbohydrate epitopes in system, including polysialic acid, human natural killer-1 (HNK-1) carbohydrate, α2,3-sialic oligomannosides, have been investigated, those glycan backbone structures, such as Galβ1-4GlcNAc Galβ1-3GlcNAc, are not fully examined. Here we report generation mice deficient β4-galactosyltransferase-II (β4GalT-II). This...
The human natural killer-1 (HNK-1) carbohydrate comprising a sulfated trisaccharide (HSO3-3GlcAβ1-3Galβ1-4GlcNAc-) is expressed on N-linked and O-mannose-linked glycans in the nervous system involved learning memory functions. Although whole/core glycan structures carrier glycoproteins for HNK-1 epitope have been studied, biosynthetic pathway of not fully characterized. Here, using mass spectrometric analyses, we identified major glycoprotein O-linked as phosphacan developing mouse brains...
Abstract The number and subunit compositions of AMPA receptors (AMPARs), hetero- or homotetramers composed four subunits GluA1–4, in the synapse is carefully tuned to sustain basic synaptic activity. This enables stimulation-induced plasticity, which central learning memory. AMPAR tetramers have been widely believed be stable from their formation endoplasmic reticulum until proteolytic decomposition. However, by observing GluA1 GluA2 at level single molecules, we find that homo-...