A5091621177- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Sarcoma Diagnosis and Treatment
- Extracellular vesicles in disease
- Acute Myeloid Leukemia Research
- Cell death mechanisms and regulation
- Chronic Myeloid Leukemia Treatments
- Cancer Mechanisms and Therapy
- Veterinary Oncology Research
- earthquake and tectonic studies
- RNA Interference and Gene Delivery
- Peroxisome Proliferator-Activated Receptors
- Phagocytosis and Immune Regulation
- Protein Kinase Regulation and GTPase Signaling
- Geological and Geophysical Studies
- Histone Deacetylase Inhibitors Research
- Ferroptosis and cancer prognosis
- Autophagy in Disease and Therapy
- Cardiac Ischemia and Reperfusion
- Caveolin-1 and cellular processes
- Vascular Tumors and Angiosarcomas
Gifu University
2016-2025
Comprehensive Blood & Cancer Center
2022
U-M Rogel Cancer Center
2022
Roswell Park Comprehensive Cancer Center
2022
Yokohama City University
2022
Tokyo Medical University
2022
Abstract Macrophages are polarized into functional classically activated and alternatively (M2) phenotypes depending on their microenvironment, these cells play an important role in the immune system. M2-like polarization of tumor-associated macrophages (TAMs) is by various secretions from cancer cells; however, interaction between TAMs not well understood. Recent studies showed that cell–derived extracellular vesicles (EVs) contribute to tumor development modulation microenvironment. In...
Serine and arginine rich splicing factor 3 (SRSF3), an SR-rich family protein, has oncogenic function in various kinds of cancer. However, the detailed mechanism had not been previously clarified. Here, we showed that SRSF3 splicer regulated expression profile pyruvate kinase, which is one rate-limiting enzymes glycolysis. Most cancer cells express kinase muscle 2 (PKM2) dominantly to maintain a glycolysis-dominant energy metabolism. Overexpression SRSF3, as well another splicer,...
// Kohei Taniguchi 1, 2 , Miku Sakai 1 Nobuhiko Sugito Minami Kumazaki Haruka Shinohara Nami Yamada Tatsushi Nakayama Hiroshi Ueda Yoshihito Nakagawa 3 Yuko Ito 4 Manabu Futamura 5 Bunji Uno Yoshinori Otsuki Kazuhiro Yoshida Kazuhisa Uchiyama Yukihiro Akao United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, 501-1193, Japan Department General Gastroenterological Surgery, Osaka College, Takatsuki, 569-8686, Gastroenterology, Fujita Health Medicine,...
The Warburg effect is a well-known feature in cancer-specific metabolism. We previously reported on the role of microRNA (miR)-145 as tumor-suppressor human bladder cancer (BC) cells. In this study, we reveal that miR-145 decreases by silencing KLF4 BC expression levels were significantly lower clinical samples and cell lines compared to those normal tissues HUC Luciferase assay results showed directly bound 3'UTR KLF4, which was shown be overexpressed using Western blot analysis...
Abstract The Warburg effect is a well-known feature of cancer cells. However, its' functional significance hasn't been elucidated yet. Pyruvate kinase muscle (PKM), which rate-limiting glycolytic enzyme, has 2 isoforms, PKM1 and PKM2. It reported that PKM2 tumor-specific isoform promotes the effect. Also, it thought tumor cells switch their PKM from to during development. Here, we showed this switching machinery was induced only in limited cases, based on expression normal tissues...
microRNA-143 (miR-143) is a well-known tumor suppressive microRNA that exhibits anti-tumoral function by targeting KRAS signaling pathways in various malignancies. We hypothesized miR-143 suppresses breast cancer progression and its effector molecules. further high expression of associated with favorable immune microenvironment estrogen receptor (ER)-positive patients which result improved survival. Two major publicly available cohorts; The Cancer Genome Atlas (TCGA) Molecular Taxonomy...
Bladder cancer is one of the most difficult malignancies to control. We explored use a novel RNA-interference method for driver oncogene regulating specific energy metabolism by combination treatment with small interfering RNA (siRNA) and microRNA. After transfection T24 253JB-V cells miR-145 and/or siR-PTBP1, we examined effects cell growth gene expression performing trypan blue dye exclusion test, Western blot, Hoechst 33342 staining, reverse transcription polymerase chain reaction...
Non-alcoholic steatohepatitis (NASH) has pathological characteristics similar to those of alcoholic hepatitis, despite the absence a drinking history. The greatest threat associated with NASH is its progression cirrhosis and hepatocellular carcinoma. pathophysiology not fully understood date. In this study, we investigated from perspective glycolysis Warburg effect, particular focus on microRNA regulation in liver-specific macrophages, also known as Kupffer cells. We established rat mouse...
Despite considerable research on K‐Ras inhibitors, none had been established until now. We synthesized nuclease‐resistant synthetic miR‐143 (miR‐143#12), which strongly silenced K‐Ras, its effector signal molecules AKT and ERK, the activator Sos1. examined anti‐proliferative effect of miR‐143#12 mechanism in human colon cancer DLD‐1 cell (G13D) other types harboring mutations. Cell growth was markedly suppressed a concentration‐dependent manner by (IC 50 : 1.32 nmol L −1 ) with decrease mRNA...
Malignant endothelial proliferative diseases including human angiosarcoma (AS) and canine hemangiosarcoma (HSA) are serious with a grave prognosis. Establishing liquid biopsy-based biomarkers for screening has definite clinical utility; however, plasma miRNAs up- or down-regulated in these sarcomas have been unclear. For identifying possible diagnostic sarcomas, we investigated whether miR-214 miR-126, which play important roles angiogenesis tumorigenesis, were elevated malignant diseases....
MicroRNA-214 regulates both angiogenic function in endothelial cells and apoptosis various cancers. However, the regulation of miR-214 is unclear canine hemangiosarcoma, which a spontaneous model human angiosarcoma. The expression functional roles hemangiosarcoma were presently explored by performing miRNA TaqMan qRT-PCR transfecting with synthetic microRNA. Here, we report that was significantly down-regulated cell lines used clinical samples hemangiosarcoma. Restoration reduced growth...
Human DEAD-box RNA helicase gene DDX6 was cloned from B-cell lymphoma cell line RC-K8. Previously, we reported that acts as oncogene in several cancers such colorectal cancer and hepatocellular carcinoma. However, the detailed mechanism of action carcinogenesis is largely unknown. In this study, examined functions clinical gastric (GC) samples GC cells. protein expression levels were higher than those adjacent normal tissues 25 (median value: 1.4 times higher). Also, results an...
Mitochondrial electron transport chain complex I (ETCC1) is the essential core of cancer metabolism, yet potent ETCC1 inhibitors capable safely suppressing tumor growth and metastasis in vivo are limited. From a plant extract screening, we identified petasin (PT) as highly inhibitor with chemical structure distinct from conventional inhibitors. PT had at least 1700 times higher activity than that metformin or phenformin induced cytotoxicity against broad spectrum types. administration also...
Abstract It has been well established that micro RNA (miR)‐143 is downregulated in human bladder cancer ( BC ). Recent precision medicine shown mutations are frequently observed FGFR 3, RAS and PIK 3 CA genes, all of which correlate with signaling networks. We have previously miR‐143 suppresses cell growth by inhibiting networks several cancers including . In the present study, we showed synthetic negatively regulated ‐binding protein Musashi‐2 MSI 2) lines. 2 an regulates stability certain...
Diet is a crucial factor for preventing most diseases. Edible plant extracts are known to contain exosome-like nanoparticles, in which food-derived microRNAs included and may serve as novel functional component human health. Here, we demonstrated that hvu-MIR168-3p the nanoparticles of rice aleurone cells down-regulated expression genes related mitochondrial electron transport chain complex I cells. Subsequently, enhanced protein RNA levels glucose transporter caused decrease blood level,...
We investigated whether miR143#12, a synthesized chemically modified miR-143-3p derivative, exerts therapeutic effects on acute myocardial infarction (AMI). Sprague–Dawley rats and Japanese white rabbits underwent 30 min of coronary occlusion followed by 2 weeks reperfusion. The rat AMI model was intravenously administered with control miRNA (9 μg/kg), 3 μg/kg or 9 miR143#12 1 h after reperfusion, while the rabbit μg/kg) miR143#12. In models, significantly reduced infarct sizes improved...
Rhabdomyosarcoma (RMS) is a soft tissue sarcoma and most frequently found in children. In RMS, there are two major subtypes, that is, embryonal RMS alveolar (ARMS). ARMS has exclusively the worse prognosis caused by formation of chimeric PAX3-FOXO1 gene. Regarding cancer, Warburg effect known as feature cancer-specific metabolism. Polypyrimidine tract-binding protein 1 (PTBP1), splicer pyruvate kinase muscle (PKM) mRNA, positive regulator energy We investigated expression effects...
Pyruvate kinase is known as the glycolytic enzyme catalyzing final step in glycolysis. In mammals, two different forms of it exist, i.e., pyruvate M1/2 (PKM) and L/R (PKLR). Also, PKM has isoforms, PKM1 PKM2. These genes have tissue-specific distribution. Namely, distributed high-energy-demanding organs, such brain muscle. PKM2 various other colon. On hand, PKLR liver red blood cells (RBCs). Interestingly, been recognized one essential for cancer-specific energy metabolism termed “Warburg...
Gastric cancer (GC) is one of the most common cancers worldwide. In clinical setting, identification HER2 overexpression in GC was a significant finding, as trastuzumab, an anti-HER2 drug, provides survival advantage to HER2-positive patients. HER2-postive GC, dysregulation PI3K/AKT and MAPK/ERK signaling pathways has been reported, inhibition these important therapeutic strategy. MiR-143 known act tumor suppressor several cancers, such bladder cancer, breast colorectal gastric cancer....