The aim of this study was to describe the prevalence and severity behavioural changes associated with age their relationship risk factors such as sex, reproductive status, bodyweight age.A cross-sectional design chosen. A total 325 geriatric dogs were included. Owners older than nine years interviewed by a veterinary behaviourist. Structured phone interviews used gather information about four categories related cognitive impairment: sleep/wake cycles, social interaction, learning house...

Animal models play a pivotal role in translational neuroscience but recurrent problems data collection, analyses, and interpretation, lack of biomarkers, tendency to over-reliance on mice have marred progress, leading one the highest attrition rates drug translation. Global initiatives improve reproducibility model selection are being implemented. Notwithstanding, still preferred animal species human brain disorders even when translation has been shown be limited. Non-human primates better...

Mutations in PINK1 (PARK6), a serine/threonine kinase involved mitochondrial homeostasis, are associated with early onset Parkinson's disease. Fibroblasts from disease patients compound heterozygous mutations exon 7 (c.1488 + 1G > A; c.1252_1488del) showed no apparent signs of impairment. To elucidate changes primarily caused by lack functional PINK1, we over-expressed wild-type which induced significant downregulation LRRK2 (PARK8). Indeed, found that protein basal levels were significantly...

Abstract Transplantation of several types stem cells (SC) for the treatment amyotrophic lateral sclerosis (ALS) has been evaluated in numerous Phase I/II clinical trials with inconclusive results. Here, we conducted a meta-analysis to systematically assess outcome SC therapy which report evolution each patient before and after cell administration. In this way, aimed determine effect intervention despite individual heterogeneity disease progression. We identified 670 references by electronic...

L-DOPA is the most effective treatment for Parkinson's disease (PD), but prolonged use leads to disabling motor complications including dyskinesia. Strong evidence supports a role of subthalamic nucleus (STN) in pathophysiology PD whereas its dyskinesia matter controversy. Here, we investigated involvement STN dyskinesia, using single-unit extracellular recording, behavioural and molecular approaches hemi-parkinsonian rats rendered dyskinetic by chronic administration. Our results show that...

Fear-related pathologies are among the most prevalent psychiatric conditions, having inappropriate learned fear and resistance to extinction as cardinal features. Exposure therapy represents a promising therapeutic approach, efficiency of which depends on inter-individual variation in learning, neurobiological basis is unknown. We characterized model whereby fear-conditioned mice were categorized (EXT)-success or EXT-failure, according their inherent ability extinguish fear. In lateral...

The relationship between humans and their pets has long fascinated researchers, particularly in exploring how attachment varies according to the type of pet. Cats dogs exhibit unique behavioral social traits that influence dynamics human–pet relationships. Moreover, specific human characteristics have been found affect this attachment. Our study examines factors pet among cat dog owners Basque Country, located northern Spain. By investigating these elements, our research aims enhance...

Mental well-being is a multifaceted concept that reflects emotional stability, psychological resilience and social connectedness. This study examines how demographic factors, perceived loneliness, support influence mental in Spain. Participants were surveyed online provided personal information along with responses to the University of California, Los Angeles (UCLA) Loneliness Scale, Medical Outcomes Study Social Support Survey (MOS-SSS), Warwick–Edinburgh Well-Being Scale (WEMWBS). Our...

Many workers contribute to the success of animal welfare and study outcomes in biomedical research. However, professional quality life (ProQoL) those who work with laboratory animals has not been explored Spain. To this end, we adapted ProQoL scale Spanish population working animals. Participants were contacted by email asked complete an anonymous on-line questionnaire. The comprised a total 498 participants, 12.4% officers/veterinarians, 19.5% caretaker/technicians, 13.9% principal...

Abstract Background The use of animals in biomedical science remains controversial. An individual’s level concern is generally influenced by their culture, previous or current experience with animals, and the specific animal species question. In this study we aimed to explore what people Spain who had never no longer worked laboratory thought mice, pigs, dogs monkeys for research purposes. We also determine whether not currently involved aforementioned felt work was justified. Results...

BACE2 is homologous to BACE1, a beta-secretase that involved in the amyloidogenic pathway of amyloid precursor protein (APP), and maps Down syndrome critical region chromosome 21. Alzheimer disease neuropathology common patients at relatively early ages, it has thus been speculated co-overexpression with APP would promote neurodegenerative phenotype. However, vivo function not yet elucidated. The aim present work analyse impact overexpression using transgenic mouse model. Our results suggest...

Brain-derived neurotrophic factor (BDNF) regulates synaptic strengthening and memory consolidation, altered BDNF expression is implicated in a number of neuropsychiatric neurodegenerative disorders. potentiates N-methyl-D-aspartate receptor function through activation Fyn ERK1/2. STriatal-Enriched protein tyrosine Phosphatase (STEP) also many the same disorders as but, contrast to BDNF, STEP opposes development strengthening. STEP-mediated dephosphorylation NMDA subunit GluN2B promotes...

Abstract Background Replacement, reduction and refinement, the 3R principles, provide a framework to minimize use suffering of animals in science. In this context, we aimed determine actual perception that individuals working with laboratory rodents biomedical research have on animal welfare their interaction animals, as well how they perceive its impact social relations. To end, designed an anonymous on-line survey for people rodents, at three responsibility levels, Spain. Results Of 356...

The information from nociceptors is processed in the dorsal horn of spinal cord by complex circuits involving excitatory and inhibitory interneurons. It well documented that GluN2B ERK1/2 phosphorylation contributes to central sensitization. Striatal-enriched protein tyrosine phosphatase (STEP) dephosphorylates ERK1/2, promoting internalization inactivation ERK1/2. activity STEP was modulated genetic (STEP knockout mice) pharmacological (recently synthesized inhibitor, TC-2153) approaches....

Abstract Background In Spain, the perceived professional quality of life among veterinarians has not been explored. Methods Veterinarians were invited to complete an online questionnaire in which they answered Professional Quality Life scale, Medical Outcomes Study Social Support Survey and Warwick‐Edinburgh Mental Well‐Being Scale. Participants asked whether receiving psychological therapy or taking anxiolytics, hypnotics antidepressant medication. Alcohol consumption was measured using Use...

Extensive literature has reported a link between stress and tumor progression, both of these factors mental health. Despite the higher incidence affective disorders in females neurochemical differences according to sex, female populations have been understudied. The aim this study was therefore analyze effect on development OF1 mice. For purpose, subjects were inoculated with B16F10 melanoma cells exposed Chronic Social Instability Stress (CSIS) model. Behavioral, neuroendocrine parameters...

In the last years, different research groups have made considerable efforts to improve care and use of animals in research. Mice (Mus musculus) are most widely used animal species European Union sociable hierarchical creatures. During experiments, researchers tend individualize males, but no consideration is given whether this social isolation causes them stress. The aim study was, therefore, explore 4 weeks could induce changes physiological parameters adult Crl:CD1(ICR) (CD1) which may...