A5071199052- Inorganic Chemistry and Materials
- Copper Interconnects and Reliability
- Ferrocene Chemistry and Applications
- Organometallic Complex Synthesis and Catalysis
- Synthesis and characterization of novel inorganic/organometallic compounds
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Cardiac tumors and thrombi
- Neurotransmitter Receptor Influence on Behavior
- Metal and Thin Film Mechanics
- Powder Metallurgy Techniques and Materials
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Cardiac electrophysiology and arrhythmias
- Nerve injury and regeneration
- Advanced Fluorescence Microscopy Techniques
- Neurogenesis and neuroplasticity mechanisms
- Cell Image Analysis Techniques
- Advanced Neuroimaging Techniques and Applications
- Multiple Sclerosis Research Studies
- Memory and Neural Mechanisms
- Bone and Joint Diseases
- Ion Channels and Receptors
- Nerve Injury and Rehabilitation
- Optical Coherence Tomography Applications
University of Florence
2015-2023
University of Trieste
2011-2014
Abstract Adult spinal cord has little regenerative potential, thus limiting patient recovery following injury. In this study, we describe a new population of cells resident in the adult rat meninges that express neural stem/precursor markers nestin and doublecortin. Furthermore, from dissociated meningeal tissue stem cell was cultured vitro subsequently shown to differentiate into functional neurons or mature oligodendrocytes. Proliferation rate number nestin- doublecortin-positive increased...
Rett Syndrome (RTT) is a neurodevelopmental disorder associated with intellectual disability, mainly caused by loss-of-function mutations in the MECP2 gene. RTT brains display decreased neuronal size and dendritic arborisation possibly either developmental failure or deficit maintenance of arbour structure. To distinguish between these two hypotheses, development Mecp2-knockout mouse hippocampal neurons was analyzed vitro. Since staging system for vitro lacking, rat compared 1-15 days (DIV)...
Tonic spiking of serotonergic neurons establishes serotonin levels in the brain. Since first observations, slow regular has been considered as a defining feature neurons. Recent studies, however, have revealed heterogeneity at multiple levels, comprising their electrophysiological properties, suggesting existence functionally distinct cellular subpopulations. In order to examine an unbiased manner whether dorsal raphe nucleus (DRN) are heterogeneous, we used non-invasive loose-seal...
G protein-activated inwardly rectifying potassium (GIRK) channels in 5-HT neurons are assumed to be principal effectors of 5-hydroxytryptamine 1A (5-HT1A) autoreceptors, but their pharmacology, subunit composition and the role regulation neuron activity have not been fully elucidated. We sought for a pharmacological tool assessing functional GIRK by characterizing effects drugs known block submicromolar range concentrations. Whole-cell voltage-clamp recording brainstem slices were used...
Brain serotonin (5-hydroxytryptamine, 5-HT) system dysfunction is implicated in exaggerated fear responses triggering various anxiety-, stress-, and trauma-related disorders. However, the underlying mechanisms are not well understood. Here, we investigated impact of constitutively inactivated 5-HT synthesis on context-dependent learning extinction using tryptophan hydroxylase 2 (Tph2) knockout mice. Fear conditioning memory paradigms were combined with c-Fos imaging electrophysiological...
Confocal detection in digital scanned laser light-sheet fluorescence microscopy (DSLM) has been established as a gold standard method to improve image quality. The selective line of complementary metal–oxide–semiconductor camera (CMOS) working rolling shutter mode allows the rejection out-of-focus and scattered light, thus reducing background signal during formation. Most modern CMOS have two shutters, but usually only single illuminating beam is used, halving maximum obtainable frame rate....
ABSTRACT Long‐term potentiation (LTP) is accompanied by increased spine density and dimensions triggered signaling cascades involving activation of the neurotrophin brain‐derived neurotrophic factor (BDNF) cytoskeleton remodeling. Chemically‐induced long‐term (c‐LTP) a widely used cellular model plasticity, whose effects on spines have been poorly investigated. We induced c‐LTP bath‐application N ‐methyl‐ d ‐aspartate receptor (NMDAR) coagonist glycine or K + channel blocker...
The firing activity of serotonergic neurons in raphe nuclei is regulated by negative feedback exerted extracellular serotonin (5-HT)o acting through somatodendritic 5-HT1A autoreceptors. steady-state [5-HT]o, sensed autoreceptors, determined the balance between rates 5-HT release and reuptake. Although it well established that reuptake 5-HTo mediated transporters (SERT), mechanism has remained unclear. It also unclear how selective inhibitor (SSRI) antidepressants increase [5-HT]o suppress...
Trazodone is an antidepressant drug with considerable affinity for 5-HT1A receptors and α1-adrenoceptors which the competitive agonist antagonist, respectively. In this study, we used cell-attached or whole-cell patch-clamp recordings to characterize effects of trazodone at somatodendritic (5-HT1AARs) serotonergic neurons in rodent dorsal raphe slices. To reveal α1-adrenoceptors, baseline firing 5-HT was facilitated by applying selective α1-adrenoceptor phenylephrine various concentrations....
The hERG1 potassium channel is aberrantly over expressed in tumors and regulates the cancer cell response to integrin-dependent adhesion. We unravel a novel signaling pathway by which integrin engagement ECM protein fibronectin promotes translocation plasma membrane its association with β1 integrins, activating girdin-dependent Gαi3 proteins kinase B (Akt). By sequestering hERG1, integrins make it avoid Rab5-mediated endocytosis, where unbound channels are degraded. cycle of expression...
Pharmacological studies aimed at the development of newly synthesized drugs directed against ion channels (as well as genetic channel mutations) involve and use transfected cells. However, identification best clone, in terms transfection efficiency, is often a time consuming procedure when performed through traditional methods such manual patch-clamp. On other hand, faster techniques, for example IF, are not informative on effective biological functionality channel(s). In present work, we...
Voltage-gated potassium channel KV1.3 inhibitors have been shown to be effective in preventing T-cell proliferation and activation by affecting intracellular Ca2+ homeostasis. Here, we present the structure-activity relationship, inhibition, immunosuppressive effects of new thiophene-based with nanomolar potency on K+ current T-lymphocytes inhibition Ltk- cells. The inhibitor trans-18 inhibited -mediated phytohemagglutinin (PHA)-activated an IC50 value 26.1 nM mammalian cells 230 nM. also...
Ion channels are implicated in various diseases, including cancer, which they modulate different aspects of cancer progression. In particular, potassium often aberrantly expressed cancers, a major example being provided by hERG1. The latter is generally complexed with β1 integrin tumour cells, and such molecular complex represents new druggable hub. present study focuses on the characterization functional consequences interaction between hERG1 integrins substrates over time. To this purpose,...
In the present work, applying whole-cell patch-clamp technique in voltage clamp mode, we have investigated effects of different drugs, such as riluzole, Psora-4 and Tram-34, on potassium currents four human lymphoma cell lines. We focused outward mediated by two channels (Kv1.3 KCa3.1), which are known to play a key physiological role lymphoid cells. The were evoked ramps ranging from -120 mV +40 conductance was measured between +20 mV, both absence presence specific blockers (Kv1.3; 1 µM)...